5NWD: TNKS2

Crystal structure of TNKS2 in complex with 2-[4-(diethylamino)phenyl]-3,4-dihydroquinazolin-4-one. Determined by X-ray diffraction at 1.45 Å resolution. Released 14 Mar 2018.

Method: X-ray diffraction
Resolution: 1.45 Å
Organism: Homo sapiens
Chains: 4
Atoms: 3,812
Mol. weight: 55.83 kDa
Ligands: ZN, 9C8
Released: 14 Mar 2018

Explore 5NWD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5NWD contains 20 α-helices and 22 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	954-957	4	1
α-helix	958-959	2
α-helix	963-974	12
β-strand	992-1001	10	1
α-helix	1003-1018	16
β-strand	1026-1031	6	1
α-helix	1036-1042	7
α-helix	1046-1048	3
β-strand	1050	1	2
β-strand	1058	1	2
β-strand	1059-1062	4	1
α-helix	1065-1069	5
α-helix	1075-1077	3
β-strand	1094-1102	9	1
β-strand	1106-1111	6	1

Chain B: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	954-957	4	3
α-helix	958-959	2
α-helix	963-974	12
β-strand	992-1001	10	3
α-helix	1003-1019	17
β-strand	1026-1031	6	3
α-helix	1036-1042	7
α-helix	1046-1048	3
β-strand	1050	1	4
β-strand	1058	1	4
β-strand	1059-1062	4	3
α-helix	1065-1069	5
α-helix	1075-1077	3
β-strand	1094-1102	9	3
β-strand	1106-1111	6	3

Chain H: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	1117-1119	3
β-strand	1124-1129	6	1
α-helix	1135-1137	3
β-strand	1138-1141	4	1
α-helix	1144-1146	3
β-strand	1147-1157	11	1
α-helix	1158-1160	3

Chain I: 2 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	1116-1119	4
β-strand	1124-1129	6	3
β-strand	1138-1141	4	3
α-helix	1144-1146	3
β-strand	1147-1157	11	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Tankyrase-2	A, B	protein	191	Homo sapiens	Q9H2K2 (AlphaFold model)
Tankyrase-2	H, I	protein	49	Homo sapiens	Q9H2K2 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5NWD_1 Tankyrase-2 (chains A, B)
MHHHHHHSSGVDLGTENLYFQSMLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHRDG
GHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHK
GFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVT
LGKSFLQFSAM

Sequence of entity 2 (H, I), FASTA

>5NWD_2 Tankyrase-2 (chains H, I)
KMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEG

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2
9C8	2-[4-(diethylamino)phenyl]-3~{H}-quinazolin-4-one	C18 H19 N3 O	2

Water and common crystallization additives (GOL, SO4) are not listed.

Primary citation

2-Phenylquinazolinones as dual-activity tankyrase-kinase inhibitors. Nkizinkiko, Y., Desantis, J., Koivunen, J. et al. Sci Rep (2018) 8:1680-1680. DOI 10.1038/s41598-018-19872-3 · PubMed

Other PDB entries of the same protein (UniProt Q9H2K2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5BXO 1.33 Å, Human Tankyrase-2 in Complex with Macrocyclised Extended Peptide cp4n2m3
5BXU 1.35 Å, Human Tankyrase-2 in Complex with Macrocyclised Extended Peptide cp4n4m5
5NWG 1.4 Å, Crystal structure of TNKS2 in complex with 7-chloro-2-{4-[(2-hydroxyethyl)(methyl)amino]p…
4PNL 1.5 Å, Crystal structure of TNKS-2 in complex with DR2313.
5NVE 1.5 Å, Crystal structure of TNKS2 in complex with 2-(4-ethoxyphenyl)-3,4-dihydroquinazolin-4-one
5NWC 1.5 Å, Crystal structure of TNKS2 in complex with 2-(2-aminophenyl)-3,4-dihydroquinazolin-4-one
7CE4 1.5 Å, Tankyrase2 catalytic domain in complex with K-476
5JRT 1.53 Å, Crystal structure of the human Tankyrase 2 (TNKS2) SAM domain (DH902/924RE)
3TWR 1.55 Å, Crystal structure of ARC4 from human Tankyrase 2 in complex with peptide from human 3BP2
5C5R 1.55 Å, Crystal structure of human tankyrase-2 in complex with a pyranopyridone inhibitor
5NVF 1.55 Å, Crystal structure of TNKS2 in complex with 2-[4-(pyridin-2-yl)phenyl]-3,4-dihydroquinazol…
4TJU 1.57 Å, Crystal Structure of human Tankyrase 2 in complex with 3,4-CPQ-5-C.

Browse structure collections

About this viewer

MolViewer shows 5NWD directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.