5T01: Human c-Jun DNA binding domain homodimer

Human c-Jun DNA binding domain homodimer in complex with methylated DNA. Determined by X-ray diffraction at 1.89 Å resolution. Released 1 Mar 2017.

Method: X-ray diffraction
Resolution: 1.89 Å
Organisms: synthetic construct, Homo sapiens
Chains: 4
Atoms: 1,889
Mol. weight: 26.85 kDa
Released: 1 Mar 2017

Explore 5T01 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5T01 contains 2 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	255-310	56

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	255-312	58

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA (5'-d(pcptpcpcptpaptpgpapcptpcpgptpcpcpapt)-3')	C	DNA	19	synthetic construct
DNA (5'-D(APAPTPGPGPAP(5CM)PGPAPGPTPCPAPTPAPGPGPAP*G)-3')	D	DNA	19	synthetic construct
Transcription factor AP-1	A, B	protein	64	Homo sapiens	P05412 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>5T01_1 DNA (5'-D(P*CP*TP*CP*CP*TP*AP*TP*GP*AP*CP*TP*CP*GP*TP*CP*CP*AP*T)-3') (chains C)
TCTCCTATGACTCGTCCAT

Sequence of entity 2 (D), FASTA

>5T01_2 DNA (5'-D(*AP*AP*TP*GP*GP*AP*(5CM)P*GP*AP*GP*TP*CP*AP*TP*AP*GP*GP*AP*G)-3') (chains D)
AATGGACGAGTCATAGGAG

Sequence of entity 3 (A, B), FASTA

>5T01_3 Transcription factor AP-1 (chains A, B)
HMKAERKRMRNRIAASKSRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQK
VMNH

Primary citation

Methyl-dependent and spatial-specific DNA recognition by the orthologous transcription factors human AP-1 and Epstein-Barr virus Zta. Hong, S., Wang, D., Horton, J.R. et al. Nucleic Acids Res (2017) 45:2503-2515. DOI 10.1093/nar/gkx057 · PubMed

Other PDB entries of the same protein (UniProt P05412 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6Y3V 1.5 Å, 14-3-3 Sigma in complex with phosphorylated c-Jun peptide
5FV8 1.99 Å, Structure of cJun-FosW coiled coil complex.
1JNM 2.2 Å, Crystal Structure of the Jun/CRE Complex
8SOS 2.33 Å, Human CD1d presenting sphingomyelin C24:1 in complex with VHH nanobody 1D17
1A02 2.7 Å, Structure of the DNA binding domains of nfat, fos and jun bound to DNA
1T2K 3.0 Å, Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA
1FOS 3.05 Å, Two human C-fos:c-jun:dna complexes
1S9K 3.1 Å, Crystal Structure of Human NFAT1 and Fos-Jun on the IL-2 ARRE1 Site
1JUN NMR study of C-jun homodimer

Browse structure collections

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