Crystal structure of LbaCas13a (C2c2) bound to mature crRNA (24-nt spacer). Determined by X-ray diffraction at 1.99 Å resolution. Released 13 Sept 2017.
Explore 5W1H in 3D Show helices and sheets RCSB PDB PDBe
5W1H contains 85 α-helices and 35 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 19-25 | 7 | 1 |
| β-strand | 30-36 | 7 | 1 |
| β-strand | 45 | 1 | 1 |
| α-helix | 48-60 | 13 | |
| β-strand | 69 | 1 | 2 |
| α-helix | 80-93 | 14 | |
| α-helix | 100-104 | 5 | |
| α-helix | 106-109 | 4 | |
| α-helix | 112-127 | 16 | |
| α-helix | 133-135 | 3 | |
| α-helix | 136-138 | 3 | |
| α-helix | 143-152 | 10 | |
| α-helix | 155-163 | 9 | |
| α-helix | 172-182 | 11 | |
| β-strand | 183 | 1 | 2 |
| α-helix | 185-187 | 3 | |
| α-helix | 190-203 | 14 | |
| α-helix | 215-220 | 6 | |
| α-helix | 221-227 | 7 | |
| α-helix | 230-241 | 12 | |
| β-strand | 247-249 | 3 | 3 |
| β-strand | 255-257 | 3 | 3 |
| α-helix | 265-284 | 20 | |
| α-helix | 289-308 | 20 | |
| α-helix | 313-315 | 3 | |
| α-helix | 324-332 | 9 | |
| β-strand | 337 | 1 | 4 |
| α-helix | 340-345 | 6 | |
| α-helix | 354-385 | 32 | |
| α-helix | 395-414 | 20 | |
| α-helix | 422-424 | 3 | |
| β-strand | 425 | 1 | 4 |
| α-helix | 426-455 | 30 | |
| α-helix | 458-460 | 3 | |
| β-strand | 467-469 | 3 | 5 |
| α-helix | 472-474 | 3 | |
| α-helix | 475-477 | 3 | |
| α-helix | 483-514 | 32 | |
| β-strand | 515 | 1 | 6 |
| α-helix | 524-526 | 3 | |
| α-helix | 527 | 1 | |
| β-strand | 528 | 1 | 7 |
| α-helix | 529 | 1 | |
| β-strand | 533 | 1 | 8 |
| β-strand | 541 | 1 | 8 |
| β-strand | 545 | 1 | 7 |
| α-helix | 546-549 | 4 | |
| β-strand | 550 | 1 | 6 |
| α-helix | 553-555 | 3 | |
| α-helix | 556-561 | 6 | |
| α-helix | 562-564 | 3 | |
| α-helix | 567-570 | 4 | |
| α-helix | 575-578 | 4 | |
| α-helix | 585-599 | 15 | |
| α-helix | 618-629 | 12 | |
| α-helix | 632-642 | 11 | |
| α-helix | 645-647 | 3 | |
| α-helix | 651-662 | 12 | |
| α-helix | 668-669 | 2 | |
| α-helix | 673-674 | 2 | |
| α-helix | 675-678 | 4 | |
| α-helix | 684-689 | 6 | |
| α-helix | 706-724 | 19 | |
| α-helix | 725-729 | 5 | |
| α-helix | 733-743 | 11 | |
| α-helix | 746-748 | 3 | |
| α-helix | 780-796 | 17 | |
| α-helix | 802-813 | 12 | |
| α-helix | 823-824 | 2 | |
| α-helix | 840-858 | 19 | |
| α-helix | 860-863 | 4 | |
| α-helix | 869-872 | 4 | |
| α-helix | 873-875 | 3 | |
| α-helix | 881-883 | 3 | |
| α-helix | 888-895 | 8 | |
| α-helix | 898-908 | 11 | |
| α-helix | 912-938 | 27 | |
| β-strand | 951-952 | 2 | 9 |
| β-strand | 955-956 | 2 | 9 |
| α-helix | 957-971 | 15 | |
| α-helix | 978-980 | 3 | |
| α-helix | 985-992 | 8 | |
| β-strand | 996 | 1 | 10 |
| α-helix | 1005-1013 | 9 | |
| β-strand | 1016-1018 | 3 | 11 |
| β-strand | 1021-1022 | 2 | 11 |
| β-strand | 1027-1028 | 2 | 12 |
| β-strand | 1031-1032 | 2 | 12 |
| α-helix | 1036-1044 | 9 | |
| α-helix | 1047-1053 | 7 | |
| β-strand | 1057 | 1 | 10 |
| α-helix | 1059-1071 | 13 | |
| α-helix | 1076-1078 | 3 | |
| α-helix | 1084-1100 | 17 | |
| α-helix | 1103-1142 | 40 | |
| α-helix | 1150-1153 | 4 | |
| β-strand | 1154-1157 | 4 | 5 |
| β-strand | 1163-1166 | 4 | 5 |
| α-helix | 1168-1175 | 8 | |
| β-strand | 1184-1186 | 3 | 13 |
| β-strand | 1193-1195 | 3 | 13 |
| α-helix | 1202-1213 | 12 | |
| α-helix | 1227-1230 | 4 | |
| α-helix | 1233-1235 | 3 | |
| α-helix | 1236-1247 | 12 | |
| α-helix | 1251-1254 | 4 | |
| α-helix | 1259-1265 | 7 | |
| α-helix | 1266-1270 | 5 | |
| α-helix | 1275-1278 | 4 | |
| α-helix | 1281-1291 | 11 | |
| β-strand | 1294-1296 | 3 | 14 |
| β-strand | 1298-1307 | 10 | 15 |
| β-strand | 1318-1327 | 10 | 15 |
| β-strand | 1333-1335 | 3 | 14 |
| α-helix | 1336 | 1 | |
| β-strand | 1337-1341 | 5 | 16 |
| β-strand | 1346-1350 | 5 | 16 |
| α-helix | 1354-1365 | 12 | |
| α-helix | 1372-1375 | 4 | |
| β-strand | 1376-1377 | 2 | 14 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| LbaCas13a (C2c2) | A | protein | 1440 | Lachnospiraceae bacterium | A0A2D0TCG9 |
| mature crRNA | B | RNA | 52 | Lachnospiraceae bacterium |
>5W1H_1 LbaCas13a (C2c2) (chains A) SNAMKISKVREENRGAKLTVNAKTAVVSENRSQEGILYNDPSRYGKSRKNDEDRDRYIES RLKSSGKLYRIFNEDKNKRETDELQWFLSEIVKKINRRNGLVLSDMLSVDDRAFEKAFEK YAELSYTNRRNKVSGSPAFETCGVDAATAERLKGIISETNFINRIKNNIDNKVSEDIIDR IIAKYLKKSLCRERVKRGLKKLLMNAFDLPYSDPDIDVQRDFIDYVLEDFYHVRAKSQVS RSIKNMNMPVQPEGDGKFAITVSKGGTESGNKRSAEKEAFKKFLSDYASLDERVRDDMLR RMRRLVVLYFYGSDDSKLSDVNEKFDVWEDHAARRVDNREFIKLPLENKLANGKTDKDAE RIRKNTVKELYRNQNIGCYRQAVKAVEEDNNGRYFDDKMLNMFFIHRIEYGVEKIYANLK QVTEFKARTGYLSEKIWKDLINYISIKYIAMGKAVYNYAMDELNASDKKEIELGKISEEY LSGISSFDYELIKAEEMLQRETAVYVAFAARHLSSQTVELDSENSDFLLLKPKGTMDKND KNKLASNNILNFLKDKETLRDTILQYFGGHSLWTDFPFDKYLAGGKDDVDFLTDLKDVIY SMRNDSFHYATENHNNGKWNKELISAMFEHETERMTVVMKDKFYSNNLPMFYKNDDLKKL LIDLYKDNVERASQVPSFNKVFVRKNFPALVRDKDNLGIELDLKADADKGENELKFYNAL YYMFKEIYYNAFLNDKNVRERFITKATKVADNYDRNKERNLKDRIKSAGSDEKKKLREQL QNYIAENDFGQRIKNIVQVNPDYTLAQICQLIMTEYNQQNNGCMQKKSAARKDINKDSYQ HYKMLLLVNLRKAFLEFIKENYAFVLKPYKHDLCDKADFVPDFAKYVKPYAGLISRVAGS SELQKWYIVSRFLSPAQANHMLGFLHSYKQYVWDIYRRASETGTEINHSIAEDKIAGVDI TDVDAVIDLSVKLCGTISSEISDYFKDDEVYAEYISSYLDFEYDGGNYKDSLNRFCNSDA VNDQKVALYYDGEHPKLNRNIILSKLYGERRFLEKITDRVSRSDIVEYYKLKKETSQYQT KGIFDSEDEQKNIKKFQEMKNIVEFRDLMDYSEIADELQGQLINWIYLRERDLMNFQLGY HYACLNNDSNKQATYVTLDYQGKKNRKINGAILYQICAMYINGLPLYYVDKDSSEWTVSD GKESTGAKIGEFYRYAKSFENTSDCYASGLEIFENISEHDNITELRNYIEHFRYYSSFDR SFLGIYSEVFDRFFTYDLKYRKNVPTILYNILLQHFVNVRFEFVSGKKMIGIDKKDRKIA KEKECARITIREKNGVYSEQFTYKLKNGTVYVDARDKRYLQSIIRLLFYPEKVNMDEMIE VKEKKKPSDNNTGKGYSKRDRQQDRKEYDKYKEKKKKEGNFLSGMGGNINWDEINAQLKN
>5W1H_2 mature crRNA (chains B) AAGAUAGCCCAAGAAAGAGGGCAAUAACCAGAUAUAGCCUGGUGGUUCAGGC
Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme. Knott, G.J., East-Seletsky, A., Cofsky, J.C. et al. Nat Struct Mol Biol (2017) 24:825-833. DOI 10.1038/nsmb.3466 · PubMed
Other PDB entries of the same protein (UniProt A0A2D0TCG9), best resolution first:
5W1H is part of these collections:
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