5WRK: Mu2 subunit of the clathrin adaptor complex AP2

Mu2 subunit of the clathrin adaptor complex AP2 in complex with IRS-1 Y608 peptide. Determined by X-ray diffraction at 2.62 Å resolution. Released 6 Dec 2017.

Method: X-ray diffraction
Resolution: 2.62 Å
Organism: Rattus norvegicus
Chains: 2
Atoms: 2,005
Mol. weight: 31.87 kDa
Ligands: NI
Released: 6 Dec 2017

Explore 5WRK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5WRK contains 3 α-helices and 18 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	172-185	14	1
β-strand	191-205	15	1
β-strand	211-215	5	2
β-strand	221-222	2	1
β-strand	229-230	2	2
β-strand	262-265	4	2
α-helix	267-268	2
β-strand	270-279	10	1
β-strand	287-296	10	3
β-strand	300-309	10	3
β-strand	316-325	10	1
β-strand	330-337	8	3
β-strand	341-345	5	1
β-strand	350-359	10	1
β-strand	363-372	10	3
α-helix	384-385	2
β-strand	386-392	7	1
β-strand	403-407	5	2
α-helix	415-417	3
β-strand	419-433	15	1

Chain P: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	3-4	2	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
AP-2 complex subunit mu	A	protein	278	Rattus norvegicus	P84092 (AlphaFold model)
Insulin receptor substrate 1	P	protein	8	Rattus norvegicus	P35570 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5WRK_1 AP-2 complex subunit mu (chains A)
QIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMN
DKIVIEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSISFIPPDGEFELMRY
RTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVIC
MKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPS
GLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC

Sequence of entity 2 (P), FASTA

>5WRK_2 Insulin receptor substrate 1 (chains P)
GYMPMSPG

Ligands and cofactors

ID	Name	Formula	Copies
NI	Nickel (II) ion	Ni	2

Primary citation

IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling. Yoneyama, Y., Lanzerstorfer, P., Niwa, H. et al. Elife (2018) 7. DOI 10.7554/eLife.32893 · PubMed

Other PDB entries of the same protein (UniProt P84092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7OIQ 1.85 Å, Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (C2 crystal form)
7OFP 1.92 Å, Apo Structure of Mu2 Adaptin Subunit (Ap50) Of AP2 Clathrin Adaptor
7OHZ 2.27 Å, Crystal structure of AP2 Mu2 - FCHO2 chimera (His6-tagged)
1I31 2.5 Å, MU2 adaptin subunit (AP50) of AP2 clathrin adaptor, complexed with EGFR internalization…
2PR9 2.51 Å, Mu2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with GABAA…
6QH5 2.56 Å, AP2 clathrin adaptor mu2T156-phosphorylated core in closed conformation
5WRM 2.6 Å, Mu2 subunit of the clathrin adaptor complex AP2 in complex with IRS-1 Y658 peptide
2VGL 2.6 Å, AP2 clathrin adaptor core
3H85 2.6 Å, Molecular basis for the association of PIPKI gamma-p90 with the clathrin adaptor AP-2
7OI5 2.61 Å, Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved)
1BW8 2.65 Å, MU2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with EGFR…
1BXX 2.7 Å, MU2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with TGN38…

Browse structure collections

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