5XOJ: Xpo1p-PKI-Nup42p-Gsp1p-GTP complex

Crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex. Determined by X-ray diffraction at 2.2 Å resolution. Released 16 Aug 2017.

Method

X-ray diffraction

Resolution

2.2 Å

Organisms

Saccharomyces cerevisiae (strain AWRI796), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Homo sapiens

Chains

6

Atoms

10,250

Mol. weight

159.49 kDa

Ligands

MG, GTP

Released

16 Aug 2017

Explore 5XOJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5XOJ contains 70 α-helices and 11 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 9 β-strands

Molecules and chains

>5XOJ_1 GTP-binding nuclear protein (chains A)
MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGE
IKFDVWDTAGLEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIV
LCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVA
SP

>5XOJ_2 Exportin-1 (chains C)
MEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDNPDAWQKADQILQFSTN
PQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQKNLINKSDL
TLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAEQMTQAKAL
HLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLLRYLHWIPYRYIYETNILELLST
KFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTVLFFQNTLQQIATSVMPVTADLKAT
YANANGNDQSFLQDLAMFLTTYLARNRALLESDESLRELLLNAHQYLIQLSKIEERELFK
TTLDYWHNLVADLFYEPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGEIVREFVK
ESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTLSWAIGSISG
TMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFLKAHWNFLRTVI
LKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQTIIRDIQKTTADLQ
PQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQSTANPTLLLDSETVK
IIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMISAQVAAEGLIATKTPKVR
GLRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMTT
VVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHRVEFYKLLKVINEKSFAAFLEL
PPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKNIERMGNVPFANEFHKNYFFIFVS
ETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKISVPLYQEAEVPQGTSNQVYLSQYLAN
MLSNAFPHLTSEQIASFLSALTKQYKDLVVFKGTLRDFLVQIKEVGGDPTDYLFAEDKEN
ALMEQNRLEREKAAKIGGLLKPSELDD

>5XOJ_3 cAMP-dependent protein kinase inhibitor alpha (chains D)
MTDVETTYADFIASGRTGRRNAIHDILVSSASGNLNELALKLAGLDINKTEGEEDAQRSS
TEQSGEAQGEAAKSES

>5XOJ_4 Nup42p (chains E, F, G)
KPSAFGAPAFGSSAPINVNPPSTTSAFGAPSFGST

Ligands and cofactors

Primary citation

Crystal structure of the Xpo1p nuclear export complex bound to the SxFG/PxFG repeats of the nucleoporin Nup42p. Koyama, M., Hirano, H., Shirai, N. et al. Genes Cells (2017) 22:861-875. DOI 10.1111/gtc.12520 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P32835 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3M1I 2.0 Å, Crystal structure of yeast CRM1 (Xpo1p) in complex with yeast RanBP1 (Yrb1p) and yeast…
• 3WYG 2.15 Å, Crystal structure of Xpo1p-PKI-Gsp1p-GTP complex
• 3WYF 2.22 Å, Crystal structure of Xpo1p-Yrb2p-Gsp1p-GTP complex
• 2X19 2.8 Å, Crystal structure of Importin13 - RanGTP complex
• 9B3I 2.88 Å, Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
• 8QYZ 3.0 Å, Crystal structure of hiNES2 in complex with Xpo1 and RanGTP
• 9D45 3.1 Å, Cryo-EM structure of yeast Exportin Msn5 bound to cargo Pho4 and RanGTP
• 9DZ6 3.1 Å, Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State…
• 3ICQ 3.2 Å, Karyopherin nuclear state
• 9DXM 3.2 Å, Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State…
• 9OGB 3.25 Å, Cryo-EM structure of human exportin-1 conjugated with selinexor and bound to yeast…
• 8F1E 3.28 Å, Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) and H2A-H2B

Browse structure collections

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