6EJZ: Trp operon repressor

Tryptophan Repressor TrpR from E.coli variant S88Y with Indole-3-acetic acid as ligand. Determined by X-ray diffraction at 1.9 Å resolution. Released 6 Feb 2019.

Method: X-ray diffraction
Resolution: 1.9 Å
Organism: Escherichia coli (strain 55989 / EAEC)
Chains: 4
Atoms: 3,675
Mol. weight: 55.79 kDa
Ligands: IAC
Released: 6 Feb 2019

Explore 6EJZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6EJZ contains 24 α-helices and 0 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	9-31	23
α-helix	35-42	8
α-helix	45-63	19
α-helix	68-75	8
α-helix	79-91	13
α-helix	94-105	12

Chains B, C and D: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	9-31	23
α-helix	35-42	8
α-helix	45-63	19
α-helix	68-75	8
α-helix	79-91	13
α-helix	94-104	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Trp operon repressor	A, B, C, D	protein	116	Escherichia coli (strain 55989 / EAEC)	P0A881 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>6EJZ_1 Trp operon repressor (chains A, B, C, D)
MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEE
LLRGEMSQRELKNELGAGIATITRGSNYLKAAPVELRQWLEEVLLKSDLEHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
IAC	1H-indol-3-ylacetic acid	C10 H9 N O2	5

Water and common crystallization additives (SO4, EDO) are not listed.

Primary citation

A biosensor for the direct visualization of auxin. Herud-Sikimic, O., Stiel, A.C., Kolb, M. et al. Nature (2021). DOI 10.1038/s41586-021-03425-2

Other PDB entries of the same protein (UniProt P0A881 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6ENI 1.1 Å, Tryptophan Repressor TrpR from E.coli variant T44L S88Y with Indole-3-acetic acid as…
6ENN 1.17 Å, Tryptophan Repressor TrpR from E.coli variant T44L T81M N87G S88Y with Indole-3-acetic…
6FAL 1.2 Å, Tryptophan Repressor TrpR from E.coli with 3-Indolepropionic acid as ligand
1JHG 1.3 Å, Trp repressor mutant V58I
6ELG 1.38 Å, Tryptophan Repressor TrpR from E.coli variant M42F T44L T81I S88Y with…
6F9K 1.4 Å, Tryptophan Repressor TrpR from E.coli with 5-methyl-L-tryptophan as ligand
6ELB 1.44 Å, Tryptophan Repressor TrpR from E.coli variant M42F T44L T81M N87G S88Y with…
6EKP 1.46 Å, Tryptophan Repressor TrpR from E.coli variant T44L T81M S88Y with Indole-3-acetic acid…
3SSX 1.58 Å, E. coli trp aporeporessor L75F mutant
2OZ9 1.65 Å, E. coli TRP holorepressor, orthorhombic crystal form
6F7G 1.66 Å, Tryptophan Repressor TrpR from E.coli with 5-Methyltryptamine
3SSW 1.67 Å, E. coli trp aporepressor

Browse structure collections

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