6K71: EIF2 - eIF2B complex

eIF2 - eIF2B complex. Determined by electron microscopy at 4.3 Å resolution. Released 10 Jul 2019.

Method: Electron microscopy
Resolution: 4.3 Å
Organism: Homo sapiens
Chains: 13
Atoms: 32,589
Mol. weight: 648.2 kDa
Released: 10 Jul 2019

Explore 6K71 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6K71 contains 159 α-helices and 243 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	8-16	9
α-helix	22-32	11
α-helix	33-35	3
α-helix	47-57	11
α-helix	64-76	13
α-helix	93-105	13
α-helix	107-114	8
β-strand	124-127	4	1
α-helix	132-142	11
β-strand	149-152	4	1
β-strand	153	1	2
α-helix	162-169	8
α-helix	177	1
β-strand	178	1	2
α-helix	179	1
α-helix	183-186	4
α-helix	187-189	3
β-strand	192	1	1
β-strand	193	1	3
β-strand	199-201	3	4
β-strand	205-207	3	4
β-strand	208-209	2	5
α-helix	210-212	3
α-helix	213-222	10
β-strand	226	1	3
β-strand	227	1	6
β-strand	235	1	4
α-helix	243-245	3
β-strand	273-274	2	5
β-strand	283	1	6
β-strand	284-285	2	7
β-strand	290-291	2	7
α-helix	295-302	8

Chain B: 13 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	6-16	11
α-helix	22-31	10
α-helix	32-35	4
α-helix	47-57	11
α-helix	63-75	13
α-helix	93-115	23
β-strand	125-127	3	8
α-helix	132-140	9
β-strand	151-152	2	8
α-helix	162-169	8
β-strand	177	1	8
α-helix	180-182	3
α-helix	187-189	3
β-strand	192-196	5	8
β-strand	199-200	2	9
β-strand	206-207	2	9
β-strand	209	1	10
α-helix	213-220	8
β-strand	226-229	4	8
β-strand	235	1	9
α-helix	248-251	4
β-strand	273	1	10
β-strand	284-285	2	11
β-strand	290-291	2	11
α-helix	296-300	5

Chain C: 12 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	12-23	12
α-helix	32-42	11
α-helix	54-71	18
α-helix	77-97	21
α-helix	129-153	25
α-helix	155-157	3
β-strand	164-168	5	12
α-helix	172-184	13
β-strand	188-192	5	12
β-strand	193	1	13
α-helix	201-206	6
β-strand	214-216	3	12
β-strand	218	1	13
α-helix	222-227	6
β-strand	232	1	12
β-strand	234-235	2	14
β-strand	236-239	4	15
β-strand	245-248	4	15
α-helix	251-257	7
β-strand	266-268	3	14
β-strand	274	1	15
β-strand	306-308	3	16
β-strand	313-316	4	15
β-strand	323-327	5	14
β-strand	329-331	3	14
α-helix	337-343	7
α-helix	346-349	4

Chain D: 13 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	12-21	10
α-helix	32-40	9
α-helix	54-71	18
α-helix	79-97	19
α-helix	129-153	25
β-strand	164-168	5	17
α-helix	172-184	13
β-strand	188-192	5	17
α-helix	201-206	6
β-strand	215-216	2	17
α-helix	219-221	3
β-strand	232-235	4	17
β-strand	238-239	2	18
β-strand	245-246	2	18
β-strand	248	1	19
α-helix	251-258	8
β-strand	265-268	4	17
β-strand	274	1	18
β-strand	288	1	20
α-helix	291-293	3
β-strand	311	1	20
β-strand	313	1	19
α-helix	318-320	3
β-strand	323-325	3	17
α-helix	337-342	6
α-helix	346-348	3

Chain E: 9 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	21
α-helix	37-42	6
β-strand	51-52	2	21
β-strand	53	1	22
α-helix	63-65	3
β-strand	77	1	22
α-helix	86-92	7
β-strand	101-103	3	21
α-helix	120-122	3
β-strand	128-129	2	21
β-strand	132	1	23
β-strand	157-160	4	24
β-strand	170-171	2	24
α-helix	173-175	3
β-strand	181-182	2	25
α-helix	184-189	6
β-strand	194-196	3	24
β-strand	201	1	23
β-strand	205-207	3	21
α-helix	209-216	8
α-helix	226-228	3
β-strand	263-264	2	21
β-strand	357-358	2	26
β-strand	364	1	27
β-strand	374-375	2	26
β-strand	380	1	28
β-strand	381	1	27
β-strand	391-392	2	26
β-strand	397	1	28
β-strand	407	1	29
β-strand	408-409	2	26
β-strand	414	1	28
β-strand	424	1	29
α-helix	441-443	3

Chain F: 7 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	6-7	2	30
α-helix	38-40	3
β-strand	52-53	2	30
α-helix	63-65	3
α-helix	86-90	5
β-strand	103-104	2	30
α-helix	117-119	3
β-strand	129	1	31
β-strand	132-133	2	32
β-strand	157	1	33
β-strand	159-160	2	34
β-strand	167	1	34
β-strand	171	1	33
β-strand	181-183	3	35
α-helix	184-189	6
β-strand	193-195	3	34
β-strand	200-201	2	32
α-helix	209-215	7
β-strand	264	1	31
β-strand	357	1	36
β-strand	363-364	2	37
β-strand	374	1	36
α-helix	375-376	2
β-strand	380-381	2	37
β-strand	391	1	36
β-strand	397-398	2	37
β-strand	402-404	3	38
β-strand	408	1	39
β-strand	416	1	40
β-strand	419-421	3	38
β-strand	425	1	39
β-strand	434	1	40

Chain G: 18 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	170-172	3
α-helix	192-194	3
α-helix	205-214	10
α-helix	224-237	14
α-helix	252-255	4
α-helix	257-266	10
α-helix	268-270	3
α-helix	271-286	16
α-helix	293-308	16
α-helix	309-314	6
α-helix	315-326	12
β-strand	333-336	4	16
α-helix	342-350	9
β-strand	358-362	5	16
α-helix	370-378	9
β-strand	383-387	5	16
α-helix	388-393	6
β-strand	400-403	4	16
β-strand	407	1	41
β-strand	408	1	42
β-strand	414-417	4	41
α-helix	418-420	3
α-helix	422-426	5
β-strand	434-437	4	16
α-helix	440-442	3
β-strand	443	1	42
β-strand	483-484	2	12
β-strand	489-492	4	41
β-strand	499-501	3	16
β-strand	508	1	16
α-helix	513-516	4

Chain H: 15 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	175-177	3
α-helix	192-194	3
α-helix	205-214	10
α-helix	224-237	14
α-helix	253-266	14
α-helix	271-286	16
α-helix	293-308	16
α-helix	309-314	6
α-helix	315-326	12
β-strand	333-334	2	43
α-helix	342-350	9
β-strand	358-360	3	43
α-helix	370-378	9
β-strand	383-385	3	43
α-helix	388-390	3
β-strand	400-401	2	43
β-strand	403	1	44
β-strand	407-408	2	45
β-strand	414-416	3	45
α-helix	422-428	7
β-strand	436-437	2	44
β-strand	457	1	46
β-strand	487	1	46
β-strand	490-492	3	45
β-strand	499-502	4	44
β-strand	505-508	4	44
α-helix	509-511	3
α-helix	513-517	5

5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Translation initiation factor eIF-2B subunit alpha	A, B	protein	305	Homo sapiens	Q14232 (AlphaFold model)
Translation initiation factor eIF-2B subunit beta	C, D	protein	351	Homo sapiens	P49770 (AlphaFold model)
Translation initiation factor eIF-2B subunit gamma	E, F	protein	452	Homo sapiens	Q9NR50 (AlphaFold model)
Translation initiation factor eIF-2B subunit delta	G, H	protein	523	Homo sapiens	Q9UI10 (AlphaFold model)
Translation initiation factor eIF-2B subunit epsilon	I, J	protein	721	Homo sapiens	Q13144
Eukaryotic translation initiation factor 2 subunit 1	K	protein	315	Homo sapiens	P05198
Eukaryotic translation initiation factor 2 subunit 2	M	protein	333	Homo sapiens	P20042
Eukaryotic translation initiation factor 2 subunit 3	P	protein	472	Homo sapiens	P41091

Sequence of entity 1 (A, B), FASTA

>6K71_1 Translation initiation factor eIF-2B subunit alpha (chains A, B)
MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVD
SSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIK
DGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLD
AAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFP
LNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDEL
IKLYL

Sequence of entity 2 (C, D), FASTA

>6K71_2 Translation initiation factor eIF-2B subunit beta (chains C, D)
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMEL
IRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNE
DFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKE
AARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTIL
ANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEG
DILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

Sequence of entity 3 (E, F), FASTA

>6K71_3 Translation initiation factor eIF-2B subunit gamma (chains E, F)
MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTTRDVQ
KALCAEFKMKMKPDIVCIPDDADMGTADSLRYIYPKLKTDVLVLSCDLITDVALHEVVDL
FRAYDASLAMLMRKGQDSIEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFMANEADLDEEL
VIKGSILQKHPRIRFHTGLVDAHLYCLKKYIVDFLMENGSITSIRSELIPYLVRKQFSSA
SSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNACRGDRWEDLSRSQVRC
YVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVGVDSLIGP
ETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEGSNIQGSVICNNAVIEKGAD
IKDCLIGSGQRIEAKAKRVNEVIVGNDQLMEI

Sequence of entity 4 (G, H), FASTA

>6K71_4 Translation initiation factor eIF-2B subunit delta (chains G, H)
MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPET
GSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKG
EQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLF
SHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDY
TTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSEL
RAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFRVVV
VDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGT
AQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQNHA
SLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

Sequence of entity 5 (I, J), FASTA

>6K71_5 Translation initiation factor eIF-2B subunit epsilon (chains I, J)
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPIS
KDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVV
RIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSV
MTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEV
RYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARV
SNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENV
LLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVK
ERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKM
KGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDS
RGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVV
LEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALG
ISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSED
D

Sequence of entity 6 (K), FASTA

>6K71_6 Eukaryotic translation initiation factor 2 subunit 1 (chains K)
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSIN
KLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLE
YTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNI
NRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTT
TLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEV
DGDDDAEEMEAKAED

Sequence of entity 7 (M), FASTA

>6K71_7 Eukaryotic translation initiation factor 2 subunit 2 (chains M)
MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEED
TRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIM
LGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELL
NRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFL
LAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFL
QCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Sequence of entity 8 (P), FASTA

>6K71_8 Eukaryotic translation initiation factor 2 subunit 3 (chains P)
MAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINIGTIGHVAHGKSTVVKA
ISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECYRSCGSSTPDEFPTDIPGT
KGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIM
KLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEGAPIIPISAQLKYNIEVVCEYIV
KKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAGGSILKGVLKVGQEIEVRPGI
VSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRADRMVGQVLGA
VGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV
KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD

Primary citation

Structural basis for eIF2B inhibition in integrated stress response. Kashiwagi, K., Yokoyama, T., Nishimoto, M. et al. Science (2019) 364:495-499. DOI 10.1126/science.aaw4104 · PubMed

Other PDB entries of the same protein (UniProt Q14232 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9Y4B 2.1 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
9ZUZ 2.25 Å, Translation Initiation Factor eIF-2B complex with the Isohexide Diamine GSK735
7VLK 2.27 Å, eIF2B-SFSV NSs C2-imposed
9Y3U 2.4 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
9Y4W 2.4 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7F64 2.42 Å, eIF2B-SFSV NSs
9Y3T 2.5 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7RLO 2.6 Å, Structure of the human eukaryotic translation initiation factor 2B (eIF2B) in complex…
3ECS 2.65 Å, Crystal structure of human eIF2B alpha
7KMA 2.7 Å, Crystal structure of eif2Balpha with a ligand.
9HVE 2.7 Å, Native human eIF2-eIF2B complex
7F66 2.76 Å, eIF2B-SFSV NSs-1-eIF2

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