6K72: EIF2(aP) - eIF2B complex

eIF2(aP) - eIF2B complex. Determined by electron microscopy at 4.6 Å resolution. Released 10 Jul 2019.

Method: Electron microscopy
Resolution: 4.6 Å
Organism: Homo sapiens
Chains: 14
Atoms: 32,731
Mol. weight: 684.36 kDa
Released: 10 Jul 2019

Explore 6K72 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6K72 contains 149 α-helices and 244 β-strands across 14 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	7-14	8
α-helix	22-33	12
α-helix	43-49	7
α-helix	50-57	8
α-helix	64-74	11
α-helix	95-115	21
α-helix	119-120	2
β-strand	125-127	3	1
α-helix	134-143	10
β-strand	150-153	4	1
α-helix	161-168	8
α-helix	169-171	3
β-strand	176-178	3	1
α-helix	183-189	7
β-strand	193-195	3	2
β-strand	199-200	2	3
β-strand	206-208	3	3
α-helix	213-221	9
α-helix	224-225	2
β-strand	226-229	4	2
α-helix	232-234	3
β-strand	235	1	3
α-helix	243-245	3
α-helix	248-250	3
β-strand	274-275	2	3
β-strand	283-285	3	2
β-strand	291	1	2
α-helix	295-301	7

Chain B: 14 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	7-15	9
α-helix	22-32	11
α-helix	43-45	3
α-helix	46-50	5
α-helix	51-56	6
α-helix	64-76	13
α-helix	95-115	21
α-helix	120	1
β-strand	125-127	3	4
α-helix	134-142	9
β-strand	150-153	4	4
α-helix	161-170	10
β-strand	175-178	4	4
α-helix	183-186	4
β-strand	192-196	5	4
β-strand	199-200	2	5
β-strand	206-208	3	5
α-helix	212-222	11
β-strand	226-229	4	4
β-strand	235	1	5
α-helix	248-250	3
β-strand	274-275	2	5
β-strand	283-286	4	4
β-strand	289-291	3	4
α-helix	296-302	7

Chain C: 14 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	31-45	15
α-helix	54-71	18
α-helix	77-97	21
α-helix	129-144	16
α-helix	147-153	7
β-strand	164-168	5	6
α-helix	172-181	10
β-strand	188-192	5	6
α-helix	200-207	8
α-helix	208-210	3
β-strand	214-217	4	6
α-helix	222-225	4
β-strand	232-234	3	7
β-strand	238-239	2	8
β-strand	245-248	4	8
α-helix	251-259	9
β-strand	265-267	3	7
β-strand	274	1	8
β-strand	288	1	9
α-helix	291-293	3
α-helix	303-305	3
β-strand	306-308	3	10
β-strand	311	1	9
β-strand	313-316	4	8
β-strand	323-324	2	7
α-helix	336-343	8

Chain D: 13 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	9-22	14
α-helix	31-44	14
α-helix	55-71	17
α-helix	77-94	18
α-helix	129-145	17
α-helix	147-155	9
β-strand	165-168	4	11
α-helix	172-181	10
β-strand	189-192	4	11
α-helix	200-206	7
β-strand	215-217	3	11
α-helix	222-225	4
β-strand	232-234	3	12
β-strand	238-240	3	13
β-strand	245-246	2	13
β-strand	248	1	14
α-helix	251-260	10
β-strand	265-267	3	12
β-strand	274-275	2	13
β-strand	288	1	15
α-helix	291-293	3
α-helix	301-303	3
β-strand	311	1	15
β-strand	313	1	14
β-strand	316	1	13
β-strand	323-324	2	12
α-helix	337-341	5

Chain E: 8 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	16
β-strand	7	1	17
β-strand	30	1	18
β-strand	34	1	18
α-helix	37-44	8
β-strand	53-54	2	19
α-helix	68-70	3
β-strand	77-78	2	19
α-helix	88-93	6
β-strand	101-103	3	16
β-strand	104	1	17
α-helix	115-123	9
β-strand	156	1	20
β-strand	158-161	4	21
β-strand	167	1	21
β-strand	172	1	20
β-strand	181-182	2	22
α-helix	184-188	5
β-strand	192-196	5	21
β-strand	205-207	3	16
α-helix	209-217	9
α-helix	224-226	3
α-helix	284-287	4
β-strand	350	1	23
β-strand	364	1	24
β-strand	366	1	23
β-strand	381	1	24
β-strand	385	1	25
β-strand	392	1	26
β-strand	397-398	2	27
β-strand	402	1	25
β-strand	409	1	26
β-strand	414-415	2	27
β-strand	419	1	25
β-strand	421	1	28
β-strand	437	1	28

Chain F: 7 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	6-7	2	29
α-helix	39-44	6
β-strand	53-54	2	30
α-helix	62-65	4
β-strand	77-78	2	30
α-helix	87-93	7
β-strand	101-104	4	29
α-helix	115-123	9
β-strand	132-133	2	31
β-strand	157-160	4	32
β-strand	167-171	5	32
β-strand	181-182	2	33
α-helix	184-189	6
β-strand	192-196	5	32
β-strand	200-201	2	31
β-strand	205-207	3	29
α-helix	209-217	9
α-helix	284-287	4
β-strand	352	1	34
β-strand	369	1	34
β-strand	374	1	35
β-strand	381	1	36
β-strand	385	1	37
β-strand	391-392	2	35
β-strand	396-397	2	38
β-strand	398	1	36
β-strand	402	1	37
β-strand	408-409	2	35
β-strand	413-414	2	38
β-strand	419-420	2	39
β-strand	435-436	2	39

Chain G: 17 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	178-180	3
α-helix	205-213	9
α-helix	222-239	18
α-helix	241-243	3
α-helix	248-266	19
α-helix	271-284	14
α-helix	293-308	16
α-helix	309-314	6
α-helix	315-323	9
α-helix	324-326	3
β-strand	329-335	7	10
α-helix	341-350	10
β-strand	355-362	8	10
α-helix	370-379	10
β-strand	383-387	5	10
α-helix	388-390	3
α-helix	391-394	4
β-strand	400-401	2	10
β-strand	407-408	2	40
β-strand	414-415	2	40
β-strand	416-417	2	41
α-helix	420-428	9
β-strand	434	1	10
β-strand	435-437	3	42
β-strand	443	1	40
α-helix	460-462	3
β-strand	482-484	3	6
β-strand	489-490	2	41
β-strand	499-502	4	42
β-strand	505-508	4	42
α-helix	509-517	9

Chain H: 12 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	205-216	12
α-helix	222-239	18
α-helix	249-266	18
α-helix	272-286	15
α-helix	293-309	17
α-helix	310-314	5
α-helix	315-323	9
β-strand	333-335	3	43
α-helix	340-350	11
β-strand	358-362	5	43
α-helix	370-378	9
β-strand	385-387	3	43
α-helix	388-390	3
β-strand	401-402	2	43
β-strand	407-409	3	44
β-strand	413-415	3	44
β-strand	416	1	45
α-helix	420-428	9
β-strand	434-435	2	43
β-strand	436-437	2	46
β-strand	443	1	44
β-strand	457	1	47
β-strand	483-485	3	11
β-strand	487	1	47
β-strand	490	1	45
β-strand	499-502	4	46
β-strand	505-508	4	46
α-helix	512-517	6

6 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Translation initiation factor eIF-2B subunit alpha	A, B	protein	305	Homo sapiens	Q14232 (AlphaFold model)
Translation initiation factor eIF-2B subunit beta	C, D	protein	351	Homo sapiens	P49770 (AlphaFold model)
Translation initiation factor eIF-2B subunit gamma	E, F	protein	452	Homo sapiens	Q9NR50 (AlphaFold model)
Translation initiation factor eIF-2B subunit delta	G, H	protein	523	Homo sapiens	Q9UI10 (AlphaFold model)
Translation initiation factor eIF-2B subunit epsilon	I, J	protein	721	Homo sapiens	Q13144
Eukaryotic translation initiation factor 2 subunit 1	K, L	protein	315	Homo sapiens	P05198
Eukaryotic translation initiation factor 2 subunit 2	M	protein	333	Homo sapiens	P20042
Eukaryotic translation initiation factor 2 subunit 3	P	protein	472	Homo sapiens	P41091

Sequence of entity 1 (A, B), FASTA

>6K72_1 Translation initiation factor eIF-2B subunit alpha (chains A, B)
MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVD
SSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIK
DGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLD
AAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFP
LNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDEL
IKLYL

Sequence of entity 2 (C, D), FASTA

>6K72_2 Translation initiation factor eIF-2B subunit beta (chains C, D)
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMEL
IRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNE
DFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKE
AARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTIL
ANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEG
DILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

Sequence of entity 3 (E, F), FASTA

>6K72_3 Translation initiation factor eIF-2B subunit gamma (chains E, F)
MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTTRDVQ
KALCAEFKMKMKPDIVCIPDDADMGTADSLRYIYPKLKTDVLVLSCDLITDVALHEVVDL
FRAYDASLAMLMRKGQDSIEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFMANEADLDEEL
VIKGSILQKHPRIRFHTGLVDAHLYCLKKYIVDFLMENGSITSIRSELIPYLVRKQFSSA
SSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNACRGDRWEDLSRSQVRC
YVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVGVDSLIGP
ETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEGSNIQGSVICNNAVIEKGAD
IKDCLIGSGQRIEAKAKRVNEVIVGNDQLMEI

Sequence of entity 4 (G, H), FASTA

>6K72_4 Translation initiation factor eIF-2B subunit delta (chains G, H)
MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPET
GSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKG
EQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLF
SHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDY
TTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSEL
RAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFRVVV
VDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGT
AQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQNHA
SLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

Sequence of entity 5 (I, J), FASTA

>6K72_5 Translation initiation factor eIF-2B subunit epsilon (chains I, J)
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPIS
KDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVV
RIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSV
MTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEV
RYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARV
SNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENV
LLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVK
ERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKM
KGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDS
RGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVV
LEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALG
ISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSED
D

Sequence of entity 6 (K, L), FASTA

>6K72_6 Eukaryotic translation initiation factor 2 subunit 1 (chains K, L)
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSIN
KLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLE
YTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNI
NRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTT
TLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEV
DGDDDAEEMEAKAED

Sequence of entity 7 (M), FASTA

>6K72_7 Eukaryotic translation initiation factor 2 subunit 2 (chains M)
MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEED
TRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIM
LGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELL
NRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFL
LAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFL
QCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Sequence of entity 8 (P), FASTA

>6K72_8 Eukaryotic translation initiation factor 2 subunit 3 (chains P)
MAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINIGTIGHVAHGKSTVVKA
ISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECYRSCGSSTPDEFPTDIPGT
KGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIM
KLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEGAPIIPISAQLKYNIEVVCEYIV
KKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAGGSILKGVLKVGQEIEVRPGI
VSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRADRMVGQVLGA
VGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV
KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD

Primary citation

Structural basis for eIF2B inhibition in integrated stress response. Kashiwagi, K., Yokoyama, T., Nishimoto, M. et al. Science (2019) 364:495-499. DOI 10.1126/science.aaw4104 · PubMed

Other PDB entries of the same protein (UniProt Q14232 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9Y4B 2.1 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
9ZUZ 2.25 Å, Translation Initiation Factor eIF-2B complex with the Isohexide Diamine GSK735
7VLK 2.27 Å, eIF2B-SFSV NSs C2-imposed
9Y3U 2.4 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
9Y4W 2.4 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7F64 2.42 Å, eIF2B-SFSV NSs
9Y3T 2.5 Å, Eukaryotic translation initiation factor 2-B (eIF2B) with a truncation in the beta…
7RLO 2.6 Å, Structure of the human eukaryotic translation initiation factor 2B (eIF2B) in complex…
3ECS 2.65 Å, Crystal structure of human eIF2B alpha
7KMA 2.7 Å, Crystal structure of eif2Balpha with a ligand.
9HVE 2.7 Å, Native human eIF2-eIF2B complex
7F66 2.76 Å, eIF2B-SFSV NSs-1-eIF2

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