6OIJ: PDB entry 6OIJ

Muscarinic acetylcholine receptor 1-G11 protein complex. Determined by electron microscopy at 3.3 Å resolution. Released 8 May 2019.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,877
Mol. weight: 156.61 kDa
Ligands: IXO, Y01
Released: 8 May 2019

Explore 6OIJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6OIJ contains 34 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-36	24
β-strand	40-45	6	1
α-helix	52-62	11
β-strand	190-196	7	1
β-strand	199-205	7	1
α-helix	213-216	4
α-helix	217-220	4
β-strand	225-231	7	1
α-helix	247-259	13
β-strand	268-274	7	1
α-helix	276-285	10
α-helix	303-314	12
β-strand	326-328	3	1
α-helix	337-355	19

Chain B: 6 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-51	5	9
β-strand	58-63	6	10
β-strand	69-74	6	10
β-strand	78-83	6	10
β-strand	88-94	7	10
β-strand	100-105	6	11
β-strand	111-116	6	11
β-strand	121-125	5	11
β-strand	134-139	6	11
β-strand	146-153	8	12
β-strand	156-161	6	12
β-strand	165-166	2	13
β-strand	169-170	2	14
β-strand	175-176	2	14
β-strand	180-181	2	13
α-helix	186-187	2
β-strand	189-191	3	15
β-strand	198-202	5	15
β-strand	207-212	6	15
β-strand	218-223	6	15
β-strand	229-234	6	16
β-strand	240-245	6	16
β-strand	249-254	6	16
β-strand	260-264	5	16
α-helix	272-273	2
β-strand	275-278	4	17
β-strand	284-288	5	17
β-strand	294-298	5	17
β-strand	304-308	5	17
β-strand	317-320	4	9
β-strand	327-330	4	9
β-strand	336-339	4	9

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-21	14
α-helix	30-43	14
α-helix	53-55	3

Chain H: 1 helix, 27 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	2
β-strand	10	1	3
β-strand	18-25	8	2
α-helix	29-31	3
β-strand	33-39	7	3
β-strand	45-51	7	3
β-strand	58-60	3	3
β-strand	68-73	6	2
β-strand	78-83	6	2
β-strand	92-99	8	3
β-strand	110-111	2	3
β-strand	115-117	3	3
β-strand	141	1	4
β-strand	146	1	5
β-strand	155-158	4	6
β-strand	160	1	4
β-strand	166	1	7
β-strand	172	1	7
β-strand	174-179	6	5
β-strand	185-190	6	5
β-strand	194-195	2	5
β-strand	197	1	8
β-strand	199	1	8
β-strand	203-208	6	6
β-strand	211-216	6	6
β-strand	225-231	7	5
β-strand	239	1	5
β-strand	244-245	2	5

Chain R: 16 helices, 0 β-strands

Element	Residues	Length
α-helix	24-52	29
α-helix	59-73	15
α-helix	74-78	5
α-helix	79-88	10
α-helix	95-128	34
α-helix	130-136	7
α-helix	139-167	29
α-helix	181-184	4
α-helix	186-193	8
α-helix	194-198	5
α-helix	199-225	27
α-helix	362-388	27
α-helix	398-413	16
α-helix	415-418	4
α-helix	423-431	9
α-helix	439-441	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein subunit…	A	protein	353	Homo sapiens	A0A3B3ITX3 (AlphaFold model), P29992 (AlphaFold model)
Muscarinic acetylcholine receptor M1	R	protein	363	Homo sapiens	P11229 (AlphaFold model)
Antibody fragment	H	protein	256	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768

Sequence of entity 1 (A), FASTA

>6OIJ_1 Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein subunit alpha-11 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKARRELKLLLLGTGESGKSTFIKQMRIIHGAG
YSEEDKRGFTKLVYQNIFTAMQAMIRAMETLKILYKYEQNKANALLIREVDVEKVTTFEH
QYVSAIKTLWEDPGIQECYDRRREYQLSDSAKYYLTDVDRIATLGYLPTQQDVLRVRVPT
TGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEDKILYSHLVDYFPEFDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 2 (R), FASTA

>6OIJ_2 Muscarinic acetylcholine receptor M1 (chains R)
DYKDDDDAAAQTSAPPAVSPQITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFK
VNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASQA
SVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGER
TVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAALQGSETP
GGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELG
YWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQCHHH
HHH

Sequence of entity 3 (H), FASTA

>6OIJ_3 Antibody fragment (chains H)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ

Sequence of entity 4 (B), FASTA

>6OIJ_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (G), FASTA

>6OIJ_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
IXO	4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-yn-1-aminium	C10 H17 N2 O2	1
Y01	Cholesterol hemisuccinate	C31 H50 O4	2

Primary citation

Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes. Maeda, S., Qu, Q., Robertson, M.J. et al. Science (2019) 364:552-557. DOI 10.1126/science.aaw5188 · PubMed

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