6PQP: Human TRPA1 ion channel

Cryo-EM structure of the human TRPA1 ion channel in complex with the covalent agonist BITC. Determined by electron microscopy at 3.06 Å resolution. Released 8 Jan 2020.

Method

Electron microscopy

Resolution

3.06 Å

Organism

Homo sapiens

Chains

4

Atoms

20,492

Mol. weight

545.68 kDa

Ligands

9BE, 6OU, LBN

Released

8 Jan 2020

Explore 6PQP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6PQP contains 160 α-helices and 24 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B, C and D: 40 helices, 6 β-strands

Molecules and chains

>6PQP_1 Transient receptor potential cation channel subfamily A member 1 (chains A, B, C, D)
MAKRSLRKMWRPGEKKEPQGVVYEDVPDDTEDFKESLKVVFEGSAYGLQNFNKQKKLKRC
DDMDTFFLHYAAAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLS
RGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSE
ALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLHINFMNNGK
ATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSV
DIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVN
LLLSKGAQVDIKDNFGRNFLHLTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHY
ACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGD
LHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLK
CTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTI
IRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYN
FKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNHPVCKEYLLMKWLAYGFRAH
MMNLGSYCLGLIPMTILVVNIKPGMAFNSTGIINETSDHSEILDTTNSYLIKTCMILVFL
SSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAV
YFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFS
SPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGL
AVGDIAEVQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHI
FCFLFCTGEIRQEIPNADKSLEMEILKQKYRLKDLTFLLEKQHELIKLIIQKMEIISETE
DDDSHCSFQDRFKKEQMEQRNSRWNTVLRAVKAKTHHLEPSNSLEVLFQGPAADYKDDDD
KAHHHHHHHHHH

Ligands and cofactors

Primary citation

Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel. Suo, Y., Wang, Z., Zubcevic, L. et al. Neuron (2020) 105:882. DOI 10.1016/j.neuron.2019.11.023 · PubMed

Related structures

Other PDB entries of the same protein (UniProt O75762 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 6HC8 1.9 Å, Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPA1 (313-339)
• 9VU1 2.59 Å, Structure of hTRPA1 complexed with LIQA
• 6V9V 2.6 Å, Structure of TRPA1 modified by Bodipy-iodoacetamide with bound calcium, LMNG
• 7OR0 2.64 Å, Cryo-EM structure of the human TRPA1 ion channel in complex with the antagonist 3-60,…
• 7OR1 2.64 Å, Cryo-EM structure of the human TRPA1 ion channel in complex with the antagonist 3-60,…
• 9MOE 2.7 Å, Preclinical and clinical evaluation of a novel TRPA1 antagonist LY3526318
• 6PQQ 2.81 Å, Cryo-EM structure of human TRPA1 C621S mutant in the apo state
• 6PQO 2.88 Å, Cryo-EM structure of the human TRPA1 ion channel in complex with the covalent agonist…
• 6X2J 3.0 Å, Structure of human TRPA1 in complex with agonist GNE551
• 7JUP 3.05 Å, Structure of human TRPA1 in complex with antagonist compound 21
• 6V9W 3.1 Å, Structure of TRPA1 (ligand-free) with bound calcium, LMNG
• 9M8S 3.1 Å, Cryo-EM structure of the human TRPA1 ion channel in ligand-free state.

Browse structure collections

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