6PSQ: DNA-directed RNA polymerase subunit beta'
Escherichia coli RNA polymerase closed complex (TRPc) with TraR and rpsT P2 promoter. Determined by electron microscopy at 3.4 Å resolution. Released 25 Mar 2020.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organism
- Escherichia coli
- Chains
- 10
- Atoms
- 32,777
- Mol. weight
- 562.9 kDa
- Ligands
- MG, 1N7, ZN
- Released
- 25 Mar 2020
Explore 6PSQ in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6PSQ contains 173 α-helices and 228 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain G: 5 helices, 21 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-18 | 6 | 34 |
| β-strand | 23 | 1 | 35 |
| β-strand | 24-31 | 8 | 34 |
| α-helix | 35-49 | 15 | |
| β-strand | 52-53 | 2 | 36 |
| β-strand | 56-59 | 4 | 37 |
| β-strand | 74 | 1 | 38 |
| α-helix | 78-85 | 8 | |
| β-strand | 90-92 | 3 | 39 |
| β-strand | 97-105 | 9 | 37 |
| β-strand | 108-111 | 4 | 38 |
| α-helix | 112-114 | 3 | |
| β-strand | 115-116 | 2 | 37 |
| β-strand | 121-123 | 3 | 39 |
| β-strand | 129-133 | 5 | 38 |
| β-strand | 139-147 | 9 | 37 |
| β-strand | 149-150 | 2 | 36 |
| β-strand | 153 | 1 | 40 |
| α-helix | 156-158 | 3 | |
| β-strand | 171-172 | 2 | 37 |
| β-strand | 175 | 1 | 40 |
| β-strand | 180-182 | 3 | 35 |
| β-strand | 185-189 | 5 | 34 |
| β-strand | 199-203 | 5 | 34 |
| β-strand | 206-207 | 2 | 35 |
| α-helix | 213-233 | 21 | |
Chain H: 6 helices, 21 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-11 | 4 | |
| β-strand | 16 | 1 | 41 |
| β-strand | 23-31 | 9 | 41 |
| α-helix | 32 | 1 | |
| α-helix | 35-49 | 15 | |
| β-strand | 53-61 | 9 | 42 |
| β-strand | 74 | 1 | 43 |
| α-helix | 79-86 | 8 | |
| β-strand | 90-91 | 2 | 44 |
| β-strand | 99-101 | 3 | 42 |
| β-strand | 104-105 | 2 | 45 |
| β-strand | 109-110 | 2 | 46 |
| β-strand | 122-123 | 2 | 44 |
| β-strand | 131-132 | 2 | 46 |
| β-strand | 133 | 1 | 43 |
| β-strand | 139-140 | 2 | 45 |
| β-strand | 142-149 | 8 | 42 |
| β-strand | 152-153 | 2 | 47 |
| α-helix | 155-157 | 3 | |
| β-strand | 172 | 1 | 42 |
| β-strand | 175-176 | 2 | 47 |
| β-strand | 180-182 | 3 | 41 |
| β-strand | 187 | 1 | 41 |
| β-strand | 190 | 1 | 48 |
| β-strand | 198 | 1 | 48 |
| β-strand | 199-207 | 9 | 41 |
| α-helix | 213-227 | 15 | |
Chain I: 55 helices, 96 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-14 | 2 | 49 |
| α-helix | 19-20 | 2 | |
| α-helix | 29-38 | 10 | |
| α-helix | 50-53 | 4 | |
| β-strand | 59-60 | 2 | 50 |
| β-strand | 66-75 | 10 | 50 |
| α-helix | 82-88 | 7 | |
| β-strand | 93-105 | 13 | 50 |
| β-strand | 114-117 | 4 | 50 |
| β-strand | 122-128 | 7 | 50 |
| β-strand | 136-137 | 2 | 51 |
| β-strand | 142-145 | 4 | 51 |
| β-strand | 147-148 | 2 | 52 |
| β-strand | 151 | 1 | 53 |
| α-helix | 152 | 1 | |
| β-strand | 154-157 | 4 | 54 |
| β-strand | 174-177 | 4 | 54 |
| β-strand | 180 | 1 | 55 |
| β-strand | 184-187 | 4 | 54 |
| β-strand | 195-198 | 4 | 54 |
| α-helix | 207-212 | 6 | |
| α-helix | 217-224 | 8 | |
| β-strand | 227-231 | 5 | 56 |
| β-strand | 238-240 | 3 | 56 |
| α-helix | 243-246 | 4 | |
| β-strand | 250 | 1 | 57 |
| β-strand | 255-257 | 3 | 58 |
| β-strand | 260-263 | 4 | 58 |
| α-helix | 267 | 1 | |
| β-strand | 268 | 1 | 57 |
| α-helix | 269-270 | 2 | |
| α-helix | 271-280 | 10 | |
| β-strand | 284-285 | 2 | 56 |
| β-strand | 296-297 | 2 | 56 |
| β-strand | 302 | 1 | 59 |
| β-strand | 309 | 1 | 59 |
| β-strand | 315-316 | 2 | 56 |
| α-helix | 319-328 | 10 | |
| β-strand | 333-336 | 4 | 56 |
| α-helix | 347-353 | 7 | |
| α-helix | 359-370 | 12 | |
| α-helix | 378-388 | 11 | |
| β-strand | 396 | 1 | 55 |
| α-helix | 399-408 | 10 | |
| α-helix | 422-437 | 16 | |
| α-helix | 442-444 | 3 | |
| β-strand | 451 | 1 | 53 |
| β-strand | 454 | 1 | 52 |
| α-helix | 456-480 | 25 | |
| α-helix | 496-507 | 12 | |
| β-strand | 512-514 | 3 | 51 |
| α-helix | 515-516 | 2 | |
| α-helix | 520-527 | 8 | |
| β-strand | 529 | 1 | 60 |
| β-strand | 530-531 | 2 | 52 |
| β-strand | 558 | 1 | 61 |
| β-strand | 574 | 1 | 60 |
| β-strand | 575 | 1 | 61 |
| β-strand | 580-581 | 2 | 62 |
| β-strand | 587-588 | 2 | 62 |
| β-strand | 589-594 | 6 | 63 |
| β-strand | 599-606 | 8 | 63 |
| α-helix | 608-611 | 4 | |
| β-strand | 616 | 1 | 63 |
| β-strand | 634-639 | 6 | 63 |
| β-strand | 641-645 | 5 | 63 |
| α-helix | 647-649 | 3 | |
| β-strand | 652-654 | 3 | 63 |
| α-helix | 657-659 | 3 | |
| α-helix | 664-666 | 3 | |
| α-helix | 671-673 | 3 | |
| β-strand | 674 | 1 | 64 |
| α-helix | 676-686 | 11 | |
| β-strand | 689 | 1 | 65 |
| α-helix | 690-691 | 2 | |
| β-strand | 701 | 1 | 49 |
| α-helix | 704-711 | 8 | |
| β-strand | 717 | 1 | 66 |
| β-strand | 722-726 | 5 | 67 |
| β-strand | 731-736 | 6 | 67 |
| β-strand | 749-752 | 4 | 67 |
| α-helix | 753-754 | 2 | |
| β-strand | 757-758 | 2 | 68 |
| β-strand | 763 | 1 | 69 |
| β-strand | 764-765 | 2 | 68 |
| β-strand | 768-769 | 2 | 70 |
| β-strand | 776-777 | 2 | 67 |
| β-strand | 782 | 1 | 66 |
| β-strand | 784-785 | 2 | 70 |
| β-strand | 789 | 1 | 71 |
| β-strand | 794 | 1 | 71 |
| β-strand | 798-804 | 7 | 72 |
| β-strand | 816-819 | 4 | 72 |
| α-helix | 820-824 | 5 | |
| β-strand | 830-841 | 12 | 69 |
| β-strand | 843 | 1 | 73 |
| β-strand | 846 | 1 | 73 |
| β-strand | 849 | 1 | 74 |
| α-helix | 859-862 | 4 | |
| β-strand | 865 | 1 | 75 |
| β-strand | 870 | 1 | 76 |
| β-strand | 871 | 1 | 75 |
| β-strand | 876-878 | 3 | 69 |
| β-strand | 882-884 | 3 | 76 |
| β-strand | 887-889 | 3 | 74 |
| α-helix | 899-905 | 7 | |
| β-strand | 913-915 | 3 | 74 |
| β-strand | 918-919 | 2 | 76 |
| α-helix | 920-921 | 2 | |
| β-strand | 925-935 | 11 | 69 |
| α-helix | 943-978 | 36 | |
| α-helix | 994-997 | 4 | |
| α-helix | 1006-1036 | 31 | |
| α-helix | 1039-1041 | 3 | |
| β-strand | 1046-1058 | 13 | 69 |
| β-strand | 1065-1067 | 3 | 77 |
| β-strand | 1073-1075 | 3 | 77 |
| β-strand | 1076-1080 | 5 | 72 |
| β-strand | 1087-1088 | 2 | 78 |
| α-helix | 1092 | 1 | |
| β-strand | 1093 | 1 | 78 |
| α-helix | 1094 | 1 | |
| β-strand | 1096-1098 | 3 | 72 |
| α-helix | 1100-1102 | 3 | |
| β-strand | 1108 | 1 | 64 |
| α-helix | 1110-1133 | 24 | |
| α-helix | 1138-1150 | 13 | |
| α-helix | 1170-1175 | 6 | |
| β-strand | 1182-1183 | 2 | 49 |
| α-helix | 1192-1201 | 10 | |
| β-strand | 1209-1210 | 2 | 72 |
| β-strand | 1212-1213 | 2 | 78 |
| α-helix | 1219 | 1 | |
| β-strand | 1220 | 1 | 78 |
| α-helix | 1221 | 1 | |
| α-helix | 1224 | 1 | |
| β-strand | 1225-1232 | 8 | 72 |
| β-strand | 1233 | 1 | 77 |
| β-strand | 1235 | 1 | 65 |
| α-helix | 1239-1241 | 3 | |
| β-strand | 1244 | 1 | 11 |
| β-strand | 1251 | 1 | 79 |
| β-strand | 1252 | 1 | 80 |
| β-strand | 1258 | 1 | 79 |
| α-helix | 1266-1267 | 2 | |
| β-strand | 1268-1270 | 3 | 10 |
| α-helix | 1273-1280 | 8 | |
| α-helix | 1284-1291 | 8 | |
| α-helix | 1300-1309 | 10 | |
| α-helix | 1321-1330 | 10 | |
| β-strand | 1335-1339 | 5 | 1 |
Chain J: 59 helices, 80 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 20-24 | 5 | 1 |
| α-helix | 27-33 | 7 | |
| β-strand | 34 | 1 | 2 |
| α-helix | 50-52 | 3 | |
| β-strand | 66 | 1 | 3 |
| β-strand | 69 | 1 | 3 |
| α-helix | 78-80 | 3 | |
| β-strand | 84 | 1 | 4 |
| β-strand | 91 | 1 | 4 |
| α-helix | 97-99 | 3 | |
| β-strand | 102 | 1 | 2 |
| β-strand | 103-112 | 10 | 5 |
| α-helix | 114-117 | 4 | |
| α-helix | 125-128 | 4 | |
| α-helix | 132-139 | 8 | |
| β-strand | 144-146 | 3 | 6 |
| β-strand | 159-160 | 2 | 6 |
| α-helix | 162-171 | 10 | |
| β-strand | 178-179 | 2 | 6 |
| α-helix | 181-191 | 11 | |
| α-helix | 194-206 | 13 | |
| α-helix | 211-229 | 19 | |
| α-helix | 234-237 | 4 | |
| β-strand | 238-244 | 7 | 5 |
| α-helix | 247-249 | 3 | |
| β-strand | 254 | 1 | 7 |
| β-strand | 260 | 1 | 7 |
| β-strand | 261-262 | 2 | 8 |
| α-helix | 264-285 | 22 | |
| α-helix | 289-307 | 19 | |
| β-strand | 317 | 1 | 9 |
| β-strand | 323 | 1 | 9 |
| α-helix | 327-331 | 5 | |
| β-strand | 345-347 | 3 | 10 |
| β-strand | 350-356 | 7 | 11 |
| β-strand | 365-369 | 5 | 12 |
| α-helix | 370-376 | 7 | |
| α-helix | 378-387 | 10 | |
| α-helix | 394-403 | 10 | |
| α-helix | 406-416 | 11 | |
| β-strand | 421-424 | 4 | 11 |
| α-helix | 431-433 | 3 | |
| β-strand | 434-437 | 4 | 11 |
| β-strand | 438-442 | 5 | 12 |
| β-strand | 446-448 | 3 | 11 |
| α-helix | 451-453 | 3 | |
| α-helix | 454-457 | 4 | |
| β-strand | 465-469 | 5 | 11 |
| α-helix | 474-483 | 10 | |
| β-strand | 485 | 1 | 11 |
| β-strand | 491 | 1 | 13 |
| β-strand | 498 | 1 | 13 |
| α-helix | 505-513 | 9 | |
| β-strand | 526-527 | 2 | 14 |
| α-helix | 530-537 | 8 | |
| β-strand | 548-556 | 9 | 14 |
| β-strand | 564-572 | 9 | 14 |
| α-helix | 574-579 | 6 | |
| α-helix | 580-582 | 3 | |
| α-helix | 589-591 | 3 | |
| α-helix | 598-612 | 15 | |
| α-helix | 615-635 | 21 | |
| α-helix | 650-669 | 20 | |
| α-helix | 675-702 | 28 | |
| β-strand | 706-707 | 2 | 15 |
| β-strand | 714-715 | 2 | 15 |
| α-helix | 721-727 | 7 | |
| α-helix | 734-740 | 7 | |
| β-strand | 748 | 1 | 16 |
| α-helix | 753 | 1 | |
| β-strand | 754 | 1 | 16 |
| α-helix | 755 | 1 | |
| α-helix | 766-767 | 2 | |
| α-helix | 769-791 | 23 | |
| α-helix | 795-804 | 10 | |
| β-strand | 809-811 | 3 | 17 |
| β-strand | 820-822 | 3 | 18 |
| β-strand | 825-826 | 2 | 19 |
| β-strand | 831-833 | 3 | 19 |
| α-helix | 835-839 | 5 | |
| β-strand | 843 | 1 | 20 |
| β-strand | 844 | 1 | 18 |
| β-strand | 848-849 | 2 | 21 |
| β-strand | 856-858 | 3 | 21 |
| β-strand | 863 | 1 | 20 |
| α-helix | 866-873 | 8 | |
| β-strand | 880-882 | 3 | 18 |
| β-strand | 894-895 | 2 | 17 |
| α-helix | 915-924 | 10 | |
| α-helix | 926-928 | 3 | |
| β-strand | 950 | 1 | 22 |
| β-strand | 956-957 | 2 | 23 |
| β-strand | 960-961 | 2 | 23 |
| β-strand | 965-966 | 2 | 24 |
| β-strand | 973 | 1 | 22 |
| β-strand | 974-975 | 2 | 24 |
| β-strand | 981-985 | 5 | 23 |
| β-strand | 991-996 | 6 | 23 |
| β-strand | 1002-1003 | 2 | 22 |
| β-strand | 1010-1011 | 2 | 23 |
| β-strand | 1018-1019 | 2 | 22 |
| β-strand | 1025 | 1 | 25 |
| β-strand | 1034-1038 | 5 | 26 |
| β-strand | 1042 | 1 | 27 |
| β-strand | 1046 | 1 | 27 |
| β-strand | 1047-1051 | 5 | 28 |
| β-strand | 1056-1060 | 5 | 28 |
| β-strand | 1061 | 1 | 29 |
| α-helix | 1062 | 1 | |
| β-strand | 1077-1081 | 5 | 26 |
| β-strand | 1089 | 1 | 30 |
| α-helix | 1090 | 1 | |
| β-strand | 1096 | 1 | 30 |
| β-strand | 1098-1100 | 3 | 26 |
| β-strand | 1105 | 1 | 29 |
| β-strand | 1106-1107 | 2 | 31 |
| α-helix | 1109-1110 | 2 | |
| β-strand | 1122-1123 | 2 | 31 |
| β-strand | 1124 | 1 | 25 |
| α-helix | 1138-1146 | 9 | |
| β-strand | 1161-1165 | 5 | 32 |
| β-strand | 1172-1178 | 7 | 32 |
| β-strand | 1187-1191 | 5 | 32 |
| α-helix | 1195-1196 | 2 | |
| β-strand | 1203-1204 | 2 | 32 |
| α-helix | 1217-1224 | 8 | |
| α-helix | 1226-1242 | 17 | |
| α-helix | 1251-1260 | 10 | |
| β-strand | 1263-1268 | 6 | 33 |
| β-strand | 1279-1281 | 3 | 33 |
| α-helix | 1282-1294 | 13 | |
| β-strand | 1301-1304 | 4 | 33 |
| α-helix | 1309-1312 | 4 | |
| α-helix | 1328-1337 | 10 | |
| α-helix | 1341 | 1 | |
| β-strand | 1342 | 1 | 1 |
| α-helix | 1343 | 1 | |
| α-helix | 1349-1352 | 4 | |
| α-helix | 1362-1372 | 11 | |
Chain K: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-13 | 7 | |
| α-helix | 17-31 | 15 | |
| α-helix | 46-56 | 11 | |
| α-helix | 61-73 | 13 | |
Chain L: 36 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-19 | 12 | |
| β-strand | 22 | 1 | 81 |
| α-helix | 26-29 | 4 | |
| α-helix | 38-50 | 13 | |
| β-strand | 55 | 1 | 81 |
| α-helix | 75-87 | 13 | |
| α-helix | 97-107 | 11 | |
| α-helix | 116-124 | 9 | |
| α-helix | 129-135 | 7 | |
| α-helix | 138-151 | 14 | |
| α-helix | 158-161 | 4 | |
| β-strand | 162 | 1 | 82 |
| α-helix | 214-232 | 19 | |
| α-helix | 245-256 | 12 | |
| β-strand | 261 | 1 | 82 |
| α-helix | 263-288 | 26 | |
| α-helix | 289-293 | 5 | |
| α-helix | 299-301 | 3 | |
| α-helix | 302-306 | 5 | |
| α-helix | 307-309 | 3 | |
| α-helix | 320-322 | 3 | |
| α-helix | 327-329 | 3 | |
| α-helix | 334-351 | 18 | |
| α-helix | 355-391 | 37 | |
| α-helix | 392-394 | 3 | |
| α-helix | 401-416 | 16 | |
| α-helix | 427-445 | 19 | |
| α-helix | 454-473 | 20 | |
| α-helix | 480-486 | 7 | |
| α-helix | 493-501 | 9 | |
| α-helix | 502-504 | 3 | |
| β-strand | 505-506 | 2 | 8 |
| α-helix | 509 | 1 | |
| β-strand | 510 | 1 | 83 |
| α-helix | 511 | 1 | |
| β-strand | 518 | 1 | 83 |
| α-helix | 519-521 | 3 | |
| β-strand | 524 | 1 | 80 |
| α-helix | 529-530 | 2 | |
| α-helix | 531-549 | 19 | |
| α-helix | 553-562 | 10 | |
| α-helix | 573-580 | 8 | |
| α-helix | 584-599 | 16 | |
| α-helix | 605-610 | 6 | |
Chain M: 4 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 256 | 1 | 84 |
| α-helix | 257-260 | 4 | |
| α-helix | 264-273 | 10 | |
| β-strand | 277 | 1 | 84 |
| α-helix | 286-289 | 4 | |
| α-helix | 297-309 | 13 | |
Chain N: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| α-helix | 42-45 | 4 | |
| α-helix | 46-51 | 6 | |
| α-helix | 59-69 | 11 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA-directed RNA polymerase subunit beta' | J | protein | 1430 | Escherichia coli | P0A8T7 (AlphaFold model) |
| Protein TraR | N | protein | 72 | Escherichia coli | P41065 (AlphaFold model) |
| DNA-directed RNA polymerase subunit alpha | G, H, M | protein | 329 | Escherichia coli | P0A7Z4 (AlphaFold model) |
| DNA-directed RNA polymerase subunit beta | I | protein | 1342 | Escherichia coli | P0A8V2 (AlphaFold model) |
| DNA-directed RNA polymerase subunit omega | K | protein | 91 | Escherichia coli | P0A800 |
| RNA polymerase sigma factor RpoD | L | protein | 616 | Escherichia coli | P00579 |
| DNA (85-mer) | O | DNA | 85 | Escherichia coli | |
| DNA (85-mer) | P | DNA | 85 | Escherichia coli | |
Sequence of entity 1 (J), FASTA
>6PSQ_1 DNA-directed RNA polymerase subunit beta' (chains J)
VKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSL
PSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKM
GAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQ
EAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKA
ISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAE
VAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYG
RDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANW
DPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKI
VDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPR
VADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQM
LRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPA
APQVTAEDASASLAELLNAGLGGSDNELELEVLFQGPSSGHHHHHHHHHH
Sequence of entity 2 (N), FASTA
>6PSQ_2 Protein TraR (chains N)
SDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPIPEARRKIFPGVTLCVEC
QAYQERQRKHYA
Sequence of entity 3 (G, H, M), FASTA
>6PSQ_3 DNA-directed RNA polymerase subunit alpha (chains G, H, M)
MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGD
VEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPV
ERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSL
TEIKDVLASRGLSLGMRLENWPPASIADE
Sequence of entity 4 (I), FASTA
>6PSQ_4 DNA-directed RNA polymerase subunit beta (chains I)
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQ
SYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD
YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVT
DEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAK
LREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE
Sequence of entity 5 (K), FASTA
>6PSQ_5 DNA-directed RNA polymerase subunit omega (chains K)
MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLIN
NQILDVRERQEQQEQEAAELQAVTAIAEGRR
Sequence of entity 6 (L), FASTA
>6PSQ_6 RNA polymerase sigma factor RpoD (chains L)
SEFMEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVME
EAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEI
DIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAEEDLAPT
ATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAREKFAELRAQYVVTRDTIKA
KGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKM
PKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQ
VKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVD
KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGRE
PTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATT
ESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRK
LRHPSRSEVLRSFLDD
Sequence of entity 7 (O), FASTA
>6PSQ_7 DNA (85-MER) (chains O)
GGCGGCGCTTATTTGCACAAATCCATTGACAAAAGAAGGCTAAAAGGGCATATTCCTCGG
CCTTTGAATTGTCCATATAGAACGC
Sequence of entity 8 (P), FASTA
>6PSQ_8 DNA (85-MER) (chains P)
GCGTTCTATATGGACAATTCAAAGGCCGAGGAATATGCCCTTTTAGCCTTCTTTTGTCAA
TGGATTTGTGCAAATAAGCGCCGCC
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| MG | Magnesium ion | Mg | 1 |
| 1N7 | Chapso | C32 H59 N2 O8 S | 4 |
| ZN | Zinc ion | Zn | 3 |
Primary citation
Stepwise Promoter Melting by Bacterial RNA Polymerase. Chen, J., Chiu, C., Gopalkrishnan, S. et al. Mol Cell (2020) 78:275. DOI 10.1016/j.molcel.2020.02.017 · PubMed
Other PDB entries of the same protein (UniProt P0A8T7 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 4IQZ 2.1 Å, The crystal structure of a large insert in RNA polymerase (RpoC) subunit from E. coli
- 8FVW 2.1 Å, CryoEM structure of E.coli transcription elongation complex bound to ppGpp
- 2AUK 2.3 Å, Structure of E. coli RNA polymerase beta' G/G' insert
- 8FVR 2.42 Å, CryoEM structure of E.coli transcription elongation complex
- 9ZP4 2.42 Å, E. coli RNA polymerase elongation complex containing the unnatural dZ:PTP base pair in a…
- 8HKC 2.49 Å, Cryo-EM structure of E. coli RNAP sigma32 complex
- 6XL5 2.5 Å, Cryo-EM structure of EcmrR-RNAP-promoter open complex (EcmrR-RPo)
- 6XL9 2.5 Å, Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA…
- 12EC 2.6 Å, Half-translocated RNA polymerase elemental paused elongation complex, swiveled (ePEC…
- 8F1J 2.6 Å, SigN RNA polymerase early-melted intermediate bound to mismatch DNA fragment dhsU36mm2…
- 8PIB 2.6 Å, autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter…
- 9MSF 2.6 Å, de novo SigN RNA polymerase transcription initiation intermediate with post-catalytic…
Browse structure collections
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