Human Brr2 Helicase Region D534C/N1866C in complex with C-tail deleted Jab1. Determined by X-ray diffraction at 2.6 Å resolution. Released 22 Jan 2020.
Explore 6S9I in 3D Show helices and sheets RCSB PDB PDBe
6S9I contains 104 α-helices and 89 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 419-421 | 3 | |
| β-strand | 435-436 | 2 | 1 |
| β-strand | 442-447 | 6 | 1 |
| β-strand | 462-463 | 2 | 2 |
| α-helix | 464-466 | 3 | |
| α-helix | 469-471 | 3 | |
| β-strand | 480-481 | 2 | 2 |
| α-helix | 483-493 | 11 | |
| β-strand | 499-502 | 4 | 3 |
| α-helix | 509-522 | 14 | |
| β-strand | 525 | 1 | 4 |
| β-strand | 531 | 1 | 4 |
| α-helix | 533-535 | 3 | |
| β-strand | 537-541 | 5 | 3 |
| α-helix | 545-559 | 15 | |
| α-helix | 560-562 | 3 | |
| β-strand | 566-568 | 3 | 3 |
| α-helix | 578-582 | 5 | |
| β-strand | 585-588 | 4 | 3 |
| α-helix | 590-597 | 8 | |
| α-helix | 606-608 | 3 | |
| β-strand | 609-614 | 6 | 3 |
| α-helix | 617-621 | 5 | |
| α-helix | 625-640 | 16 | |
| β-strand | 647-652 | 6 | 3 |
| α-helix | 658-664 | 7 | |
| β-strand | 673-675 | 3 | 3 |
| α-helix | 678-680 | 3 | |
| β-strand | 685-692 | 8 | 1 |
| α-helix | 699-713 | 15 | |
| β-strand | 721-724 | 4 | 1 |
| α-helix | 728-741 | 14 | |
| α-helix | 760-768 | 9 | |
| β-strand | 782-785 | 4 | 1 |
| α-helix | 795-801 | 7 | |
| β-strand | 808-811 | 4 | 1 |
| α-helix | 813-818 | 6 | |
| β-strand | 823 | 1 | 5 |
| β-strand | 825-828 | 4 | 1 |
| β-strand | 832-835 | 4 | 6 |
| β-strand | 840-843 | 4 | 6 |
| α-helix | 844-845 | 2 | |
| α-helix | 846-853 | 8 | |
| β-strand | 856 | 1 | 5 |
| β-strand | 865-872 | 8 | 1 |
| α-helix | 873-875 | 3 | |
| α-helix | 876-882 | 7 | |
| α-helix | 897-906 | 10 | |
| β-strand | 912 | 1 | 7 |
| α-helix | 913-920 | 8 | |
| α-helix | 924-931 | 8 | |
| α-helix | 940-943 | 4 | |
| α-helix | 950-966 | 17 | |
| β-strand | 970 | 1 | 8 |
| β-strand | 978 | 1 | 7 |
| β-strand | 981 | 1 | 8 |
| α-helix | 983-990 | 8 | |
| α-helix | 995-1004 | 10 | |
| α-helix | 1011-1018 | 8 | |
| α-helix | 1022-1024 | 3 | |
| α-helix | 1031-1033 | 3 | |
| α-helix | 1034-1042 | 9 | |
| α-helix | 1055-1068 | 14 | |
| α-helix | 1075-1102 | 28 | |
| β-strand | 1104 | 1 | 9 |
| α-helix | 1105-1120 | 16 | |
| α-helix | 1128-1131 | 4 | |
| α-helix | 1141-1144 | 4 | |
| α-helix | 1150-1152 | 3 | |
| α-helix | 1161-1164 | 4 | |
| α-helix | 1168-1170 | 3 | |
| α-helix | 1171-1178 | 8 | |
| β-strand | 1184-1192 | 9 | 10 |
| β-strand | 1197-1206 | 10 | 10 |
| α-helix | 1212-1215 | 4 | |
| β-strand | 1218-1226 | 9 | 11 |
| β-strand | 1232 | 1 | 9 |
| β-strand | 1233-1242 | 10 | 11 |
| α-helix | 1243-1245 | 3 | |
| β-strand | 1250-1257 | 8 | 10 |
| β-strand | 1265-1272 | 8 | 11 |
| β-strand | 1279-1285 | 7 | 11 |
| α-helix | 1291-1300 | 10 | |
| α-helix | 1304-1307 | 4 | |
| β-strand | 1308 | 1 | 12 |
| α-helix | 1309-1312 | 4 | |
| α-helix | 1315-1318 | 4 | |
| β-strand | 1327 | 1 | 12 |
| α-helix | 1330-1340 | 11 | |
| β-strand | 1346-1349 | 4 | 13 |
| α-helix | 1356-1371 | 16 | |
| β-strand | 1376-1379 | 4 | 13 |
| α-helix | 1383-1393 | 11 | |
| α-helix | 1394-1399 | 6 | |
| β-strand | 1405-1407 | 3 | 13 |
| α-helix | 1412-1421 | 10 | |
| β-strand | 1424-1427 | 4 | 13 |
| α-helix | 1429-1436 | 8 | |
| α-helix | 1443-1446 | 4 | |
| β-strand | 1450-1453 | 4 | 13 |
| α-helix | 1456-1460 | 5 | |
| α-helix | 1464-1481 | 18 | |
| α-helix | 1484-1485 | 2 | |
| β-strand | 1486-1491 | 6 | 13 |
| β-strand | 1494 | 1 | 14 |
| α-helix | 1497-1503 | 7 | |
| α-helix | 1508-1510 | 3 | |
| β-strand | 1511-1513 | 3 | 13 |
| α-helix | 1516-1518 | 3 | |
| β-strand | 1523-1530 | 8 | 15 |
| α-helix | 1535-1553 | 19 | |
| β-strand | 1559-1563 | 5 | 15 |
| α-helix | 1566-1582 | 17 | |
| β-strand | 1590 | 1 | 16 |
| α-helix | 1609-1614 | 6 | |
| β-strand | 1617-1620 | 4 | 15 |
| α-helix | 1626-1638 | 13 | |
| β-strand | 1641 | 1 | 16 |
| β-strand | 1643-1647 | 5 | 15 |
| α-helix | 1648-1652 | 5 | |
| β-strand | 1658 | 1 | 17 |
| β-strand | 1660-1664 | 5 | 15 |
| β-strand | 1667-1670 | 4 | 18 |
| β-strand | 1675-1678 | 4 | 18 |
| α-helix | 1679-1680 | 2 | |
| α-helix | 1681-1688 | 8 | |
| β-strand | 1691 | 1 | 17 |
| β-strand | 1700-1707 | 8 | 15 |
| α-helix | 1708-1710 | 3 | |
| α-helix | 1711-1719 | 9 | |
| α-helix | 1721 | 1 | |
| β-strand | 1722 | 1 | 14 |
| α-helix | 1723 | 1 | |
| α-helix | 1728-1730 | 3 | |
| α-helix | 1733-1741 | 9 | |
| β-strand | 1747 | 1 | 19 |
| α-helix | 1748-1755 | 8 | |
| α-helix | 1759-1766 | 8 | |
| α-helix | 1768-1771 | 4 | |
| α-helix | 1778-1798 | 21 | |
| β-strand | 1802-1805 | 4 | 19 |
| β-strand | 1809-1812 | 4 | 19 |
| α-helix | 1814-1822 | 9 | |
| α-helix | 1826-1835 | 10 | |
| α-helix | 1842-1850 | 9 | |
| α-helix | 1853-1855 | 3 | |
| α-helix | 1887-1899 | 13 | |
| α-helix | 1907-1932 | 26 | |
| α-helix | 1936-1951 | 16 | |
| α-helix | 1959-1962 | 4 | |
| α-helix | 1968-1975 | 8 | |
| α-helix | 1982-1986 | 5 | |
| α-helix | 1991-1994 | 4 | |
| α-helix | 2001-2013 | 13 | |
| β-strand | 2017-2023 | 7 | 20 |
| β-strand | 2034 | 1 | 21 |
| β-strand | 2037-2043 | 7 | 20 |
| β-strand | 2064-2070 | 7 | 22 |
| β-strand | 2075-2082 | 8 | 22 |
| β-strand | 2085 | 1 | 23 |
| β-strand | 2087 | 1 | 23 |
| β-strand | 2089-2092 | 4 | 20 |
| β-strand | 2095 | 1 | 21 |
| β-strand | 2103-2110 | 8 | 22 |
| β-strand | 2118 | 1 | 22 |
| β-strand | 2121-2123 | 3 | 22 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 2072-2080 | 9 | |
| α-helix | 2083-2088 | 6 | |
| β-strand | 2090-2092 | 3 | 24 |
| β-strand | 2103-2107 | 5 | 24 |
| α-helix | 2108-2116 | 9 | |
| β-strand | 2125-2131 | 7 | 24 |
| β-strand | 2139-2146 | 8 | 24 |
| β-strand | 2150 | 1 | 25 |
| β-strand | 2151-2152 | 2 | 26 |
| β-strand | 2157-2158 | 2 | 26 |
| α-helix | 2163-2164 | 2 | |
| α-helix | 2167-2170 | 4 | |
| β-strand | 2173-2181 | 9 | 24 |
| α-helix | 2190-2202 | 13 | |
| β-strand | 2212-2217 | 6 | 24 |
| β-strand | 2223-2230 | 8 | 24 |
| α-helix | 2232-2240 | 9 | |
| α-helix | 2253-2255 | 3 | |
| β-strand | 2256-2258 | 3 | 24 |
| β-strand | 2260-2264 | 5 | 24 |
| β-strand | 2270-2274 | 5 | 24 |
| β-strand | 2280 | 1 | 25 |
| α-helix | 2285-2287 | 3 | |
| β-strand | 2296-2298 | 3 | 24 |
| α-helix | 2300-2303 | 4 | |
| α-helix | 2307-2309 | 3 | |
| α-helix | 2311-2315 | 5 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| U5 small nuclear ribonucleoprotein 200 kDa helicase | B | protein | 1747 | Homo sapiens | O75643 (AlphaFold model) |
| Pre-mRNA-processing-splicing factor 8 | J | protein | 263 | Homo sapiens | Q6P2Q9 (AlphaFold model) |
>6S9I_1 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains B) GAEFMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPA LKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGK TNVALMCMLREIGKHINMDGTINVCDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAEL TGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLE ALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTY VGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLG LFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVL VSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGIL ITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRM LRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIAS HYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVK ESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTD KTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPK MGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVD SEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHL ILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVG APTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLT GETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEV ICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQG FNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRF LHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRS LCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGS KKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPN YYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTI ELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDCLLRQLAQKVPHKLNNPKFNDPHV KTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMV TQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVAR FCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIG DAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEA ETDSDSD
>6S9I_2 Pre-mRNA-processing-splicing factor 8 (chains J) GPLGSMTQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICI SDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGW IHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEW GRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQ LANPKEFYHEVHRPSHFLNFALL
The inactive C-terminal cassette of the dual-cassette RNA helicase BRR2 both stimulates and inhibits the activity of the N-terminal helicase unit. Vester, K., Santos, K.F., Kuropka, B. et al. J Biol Chem (2020) 295:2097-2112. DOI 10.1074/jbc.RA119.010964 · PubMed
Other PDB entries of the same protein (UniProt O75643 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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