6UIN: DNA repair protein RAD4

Role of Beta-hairpin motifs in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complex. Determined by X-ray diffraction at 3.35 Å resolution. Released 14 Oct 2020.

Method
X-ray diffraction
Resolution
3.35 Å
Organism
Saccharomyces cerevisiae
Chains
4
Atoms
5,178
Mol. weight
94.61 kDa
Released
14 Oct 2020

Explore 6UIN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6UIN contains 34 α-helices and 25 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 25 β-strands

ElementResiduesLengthSheet
β-strand13111
α-helix134-16330
α-helix165-1728
α-helix177-1837
α-helix185-1873
α-helix192-21322
β-strand216-21722
α-helix230-2367
α-helix248-25710
β-strand259-26022
α-helix262-27514
β-strand280-28673
α-helix288-2903
β-strand29511
α-helix306-3094
β-strand315-32173
β-strand326-33163
β-strand337-33933
α-helix354-3574
β-strand362-36653
β-strand372-37433
α-helix376-3794
α-helix387-3904
α-helix392-3943
α-helix396-40914
α-helix416-43116
α-helix439-4413
β-strand447-44934
α-helix450-4523
β-strand457-45935
α-helix4601
β-strand467-47044
β-strand478-48364
α-helix484-4863
β-strand487-48935
β-strand49116
α-helix493-4975
β-strand501-50337
α-helix5041
α-helix507-5093
β-strand511-51228
β-strand530-53128
β-strand53216
α-helix534-5363
β-strand537-53937
α-helix542-5454
β-strand559-56029
α-helix564-5663
β-strand571-57559
α-helix579-5868
β-strand592-59769
β-strand607-61599
α-helix616-62712
α-helix629-6313
Chain X: 5 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix259-27113
α-helix273-2753
α-helix276-28611
α-helix288-2969
α-helix298-3069

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
DNA repair protein RAD4Aprotein538Saccharomyces cerevisiaeP14736 (AlphaFold model)
UV excision repair protein RAD23Xprotein171Saccharomyces cerevisiaeP32628 (AlphaFold model)
DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*(G47)P*AP*CP*AP*TP*CP*CP*CP*CP*CP*CP*CP*TP*AP*CP*AP*A)-3')WDNA24Saccharomyces cerevisiae
DNA (5'-d(*ap*tp*tp*gp*tp*ap*gp*np*np*np*np*gp*gp*ap*tp*gp*tp*cp*gp*ap*gp*tp*cp*a)-3')YDNA24Saccharomyces cerevisiae
Sequence of entity 1 (A), FASTA
>6UIN_1 DNA repair protein RAD4 (chains A)
GSSRAMGNEVAGVEDISVEITPSSKRNSDARRTSRNCSSNEERKRRKYFHMLYLVCLMVH
GFIRNEWINSKRLSRKLSNLVPEKVFELLHPQKDEELPLRSTRKLLDGLKKCMELWQKHW
KITKKYDNEGLYMRTWKEIEMSANNKRKFKTLKRSDFLRAVSKGHGDPDISVQGFVAMLR
ACNVNARLIMSCQPPDFTNMKIDTSLNGNNAYKDMVKYPIFWCEVWDKFSKKWITVDPVN
LKTIEQVRLHSKLAPKGVACCERNMLRYVIAYDRKYGCRDVTRRYAQWMNSKVRKRRITK
DDFGEKWFRKVITALHHRKRTKIDDYEDQYFFQRDESEGIPDSVQDLKNHPYYVLEQDIK
QTQIVKPGCKECGYLKVHGKVGKVLKVYAKRDIADLKSARQWYMNGRILKTGSRCKKVIK
RTVGRPKGEAEEEDERLYSFEDTELYIPPLASASGEITKNTFGNIEVFAPTMIPGNCCLV
ENPVAIKAARFLGVEFAPAVTSFKFERGSTVKPVLSGIVVAKWLREAIETAIDGIEFI
Sequence of entity 2 (X), FASTA
>6UIN_2 UV excision repair protein RAD23 (chains X)
GSGNASSGALGTTGGATDAAQGGPPGSIGLTVEDLLSLRQVVSGNPEALAPLLENISARY
PQLREHIMANPEVFVSMLLEAVGDNMQDVMEGADDMVEGEDIEVTGEAAAAGLGQGEGEG
SFQVDYTPEDDQAISRLCELGFERDLVIQVYFACDKNEEAAANILFSDHAD
Sequence of entity 3 (W), FASTA
>6UIN_3 DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*(G47)P*AP*CP*AP*TP*CP*CP*CP*CP*CP*CP*CP*TP*AP*CP*AP*A)-3') (chains W)
TTGACTCGACATCCCCCCCTACAA
Sequence of entity 4 (Y), FASTA
>6UIN_4 DNA (5'-D(*AP*TP*TP*GP*TP*AP*GP*NP*NP*NP*NP*GP*GP*AP*TP*GP*TP*CP*GP*AP*GP*TP*CP*A)-3') (chains Y)
ATTGTAGNNNNGGATGTCGAGTCA

Primary citation

Tethering-facilitated DNA 'opening' and complementary roles of beta-hairpin motifs in the Rad4/XPC DNA damage sensor protein. Paul, D., Mu, H., Tavakoli, A. et al. Nucleic Acids Res (2021) 48:12348-12364. DOI 10.1093/nar/gkaa909

Other PDB entries of the same protein (UniProt P14736 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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