6UPL: FACT_subnucleosome complex 2
Structure of FACT_subnucleosome complex 2. Determined by electron microscopy at 7.4 Å resolution. Released 11 Dec 2019.
- Method
- Electron microscopy
- Resolution
- 7.4 Å
- Organisms
- Homo sapiens, Escherichia coli
- Chains
- 12
- Atoms
- 15,882
- Mol. weight
- 341.47 kDa
- Released
- 11 Dec 2019
Explore 6UPL in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6UPL contains 52 α-helices and 79 β-strands across 10 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 6 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-42 | 2 | |
| α-helix | 45-50 | 6 | |
| α-helix | 51-55 | 5 | |
| α-helix | 64-75 | 12 | |
| β-strand | 84 | 1 | 1 |
| α-helix | 86-113 | 28 | |
| β-strand | 118-119 | 2 | 2 |
| α-helix | 121-131 | 11 | |
Chain B: 5 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 26-28 | 3 | |
| α-helix | 31-39 | 9 | |
| β-strand | 45-46 | 2 | 2 |
| α-helix | 48-75 | 28 | |
| β-strand | 81 | 1 | 1 |
| α-helix | 84-92 | 9 | |
| α-helix | 96 | 1 | |
| β-strand | 97-98 | 2 | 3 |
Chain C: 4 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-37 | 11 | |
| α-helix | 46-73 | 28 | |
| α-helix | 80-88 | 9 | |
| α-helix | 92-96 | 5 | |
| β-strand | 100-102 | 3 | 4 |
Chain D: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 39-48 | 10 | |
| α-helix | 56-83 | 28 | |
| α-helix | 91-101 | 11 | |
| α-helix | 105-121 | 17 | |
Chain E: 4 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 45-56 | 12 | |
| α-helix | 64-78 | 15 | |
| β-strand | 83-84 | 2 | 5 |
| α-helix | 86-114 | 29 | |
| α-helix | 121-131 | 11 | |
Chain F: 3 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 31-40 | 10 | |
| α-helix | 50-75 | 26 | |
| β-strand | 80-81 | 2 | 5 |
| α-helix | 83-93 | 11 | |
| β-strand | 96-98 | 3 | 4 |
Chain G: 13 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 523-525 | 3 | |
| β-strand | 530-532 | 3 | 7 |
| β-strand | 537-541 | 5 | 7 |
| β-strand | 546-550 | 5 | 7 |
| α-helix | 551-553 | 3 | |
| β-strand | 554-560 | 7 | 8 |
| β-strand | 566-572 | 7 | 8 |
| β-strand | 593 | 1 | 9 |
| β-strand | 594-602 | 9 | 8 |
| α-helix | 617-635 | 19 | |
| α-helix | 647-649 | 3 | |
| β-strand | 661-668 | 8 | 10 |
| β-strand | 677-682 | 6 | 10 |
| β-strand | 686-691 | 6 | 10 |
| β-strand | 696-700 | 5 | 10 |
| β-strand | 704-708 | 5 | 11 |
| β-strand | 721-730 | 10 | 11 |
| β-strand | 733-738 | 6 | 11 |
| β-strand | 739-741 | 3 | 10 |
| α-helix | 764-787 | 24 | |
| β-strand | 797-798 | 2 | 11 |
| α-helix | 799-800 | 2 | |
| α-helix | 802-804 | 3 | |
| β-strand | 806 | 1 | 12 |
| β-strand | 809 | 1 | 13 |
| β-strand | 817 | 1 | 12 |
| β-strand | 818-819 | 2 | 14 |
| β-strand | 824-825 | 2 | 14 |
| α-helix | 832-833 | 2 | |
| β-strand | 840-845 | 6 | 15 |
| β-strand | 855 | 1 | 16 |
| β-strand | 857-861 | 5 | 15 |
| α-helix | 867-869 | 3 | |
| β-strand | 870 | 1 | 15 |
| β-strand | 872 | 1 | 13 |
| β-strand | 874 | 1 | 16 |
| α-helix | 879-887 | 9 | |
| α-helix | 902-909 | 8 | |
| α-helix | 913-918 | 6 | |
| α-helix | 921-925 | 5 | |
Chain H: 4 helices, 40 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7 | 1 | 17 |
| β-strand | 11-12 | 2 | 18 |
| β-strand | 20 | 1 | 18 |
| β-strand | 23-25 | 3 | 17 |
| β-strand | 30-33 | 4 | 17 |
| β-strand | 40-43 | 4 | 17 |
| β-strand | 48-54 | 7 | 19 |
| β-strand | 61-66 | 6 | 19 |
| β-strand | 71-73 | 3 | 19 |
| β-strand | 74-75 | 2 | 18 |
| α-helix | 81-89 | 9 | |
| β-strand | 110 | 1 | 9 |
| β-strand | 111-114 | 4 | 20 |
| β-strand | 119-123 | 5 | 20 |
| β-strand | 126-127 | 2 | 20 |
| β-strand | 138-140 | 3 | 8 |
| β-strand | 144 | 1 | 21 |
| β-strand | 147-149 | 3 | 8 |
| β-strand | 162-166 | 5 | 8 |
| β-strand | 167 | 1 | 21 |
| β-strand | 201-208 | 8 | 22 |
| β-strand | 211-218 | 8 | 22 |
| β-strand | 222-226 | 5 | 22 |
| β-strand | 233-235 | 3 | 22 |
| β-strand | 242-243 | 2 | 23 |
| β-strand | 244 | 1 | 22 |
| β-strand | 252-264 | 13 | 22 |
| β-strand | 269-278 | 10 | 22 |
| β-strand | 285-286 | 2 | 24 |
| α-helix | 291-297 | 7 | |
| β-strand | 305-306 | 2 | 24 |
| α-helix | 312-322 | 11 | |
| β-strand | 326-327 | 2 | 23 |
| β-strand | 329 | 1 | 25 |
| β-strand | 342-344 | 3 | 26 |
| β-strand | 349-351 | 3 | 26 |
| β-strand | 353-355 | 3 | 25 |
| β-strand | 358-360 | 3 | 25 |
| β-strand | 369 | 1 | 25 |
| β-strand | 374-380 | 7 | 27 |
| β-strand | 389-395 | 7 | 27 |
| β-strand | 400-406 | 7 | 27 |
| α-helix | 411-420 | 10 | |
| β-strand | 424-426 | 3 | 27 |
2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Histone H3.1 | A, E | protein | 136 | Homo sapiens | P68431 (AlphaFold model) |
| Histone H4 | B, F | protein | 103 | Homo sapiens | P62805 (AlphaFold model) |
| Histone H2A | C, K | protein | 130 | Homo sapiens | Q93077 (AlphaFold model) |
| Histone H2B | D, L | protein | 126 | Homo sapiens | P62807 (AlphaFold model) |
| FACT complex subunit SPT16 | G | protein | 966 | Homo sapiens | Q9Y5B9 |
| FACT complex subunit SSRP1 | H | protein | 640 | Homo sapiens | Q08945 |
| DNA (79-mer) | I | DNA | 79 | Escherichia coli | |
| DNA (79-mer) | J | DNA | 79 | Escherichia coli | |
Sequence of entity 1 (A, E), FASTA
>6UPL_1 Histone H3.1 (chains A, E)
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTI
MPKDIQLARRIRGERA
Sequence of entity 2 (B, F), FASTA
>6UPL_2 Histone H4 (chains B, F)
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
Sequence of entity 3 (C, K), FASTA
>6UPL_3 Histone H2A (chains C, K)
MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLT
AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKK
TESHHKAKGK
Sequence of entity 4 (D, L), FASTA
>6UPL_4 Histone H2B (chains D, L)
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSSK
Sequence of entity 5 (G), FASTA
>6UPL_5 FACT complex subunit SPT16 (chains G)
MHHHHHHAVTLDKDAYYRRVKRLYSNWRKGEDEYANVDAIVVSVGVDEEIVYAKSTALQT
WLFGYELTDTIMVFCDDKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLIREKNESN
KSSFDKMIEAIKESKNGKKIGVFSKDKFPGEFMKSWNDCLNKEGFDKIDISAVVAYTIAV
KEDGELNLMKKAASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGA
DPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSYCSNLVRTLMVDP
SQEVQENYNFLLQLQEELLKELRHGVKICDVYNAVMDVVKKQKPELLNKITKNLGFGMGI
EFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGP
ATVLTSVKKKVKNVGIFLKNEDEEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKR
RAHQKELAAQLNEEAKRRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKK
YETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATF
VKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQKRYKXXXXXXXXXXXXVKQDSLVIN
LNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDG
EMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRH
KLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFV
VTLDEVELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYT
EGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXX
Sequence of entity 6 (H), FASTA
>6UPL_6 FACT complex subunit SSRP1 (chains H)
MAETLEFNDVYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGH
GLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLS
FDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQXXXXXXXXX
XXXXXXXXXXXXXXXXDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVL
RLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVEKRFEG
RLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFI
YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNA
KKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDE
SFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKK
GKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKA
EDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKK
Sequence of entity 7 (I), FASTA
>6UPL_7 DNA (79-mer) (chains I)
TCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCG
CCAAGGGGATTACTCCCTA
Sequence of entity 8 (J), FASTA
>6UPL_8 DNA (79-mer) (chains J)
TAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCG
GTGCTAGAGCTGTCTACGA
Primary citation
FACT caught in the act of manipulating the nucleosome. Liu, Y., Zhou, K., Zhang, N. et al. Nature (2020) 577:426-431. DOI 10.1038/s41586-019-1820-0 · PubMed
Other PDB entries of the same protein (UniProt P68431 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 5SVY 1.05 Å, MORC3 CW in complex with histone H3K4me1
- 2V89 1.1 Å, Crystal structure of RAG2-PHD finger in complex with H3K4me3 peptide at 1.1A resolution
- 5SZC 1.19 Å, Structure of human Dpf3 double-PHD domain bound to histone H3 tail peptide with…
- 5SZB 1.2 Å, Structure of human Dpf3 double-PHD domain bound to histone H3 tail peptide with…
- 6BHD 1.25 Å, Crystal structure of SETDB1 with a modified H3 peptide
- 4UP0 1.28 Å, Ternary crystal structure of the Pygo2 PHD finger in complex with the B9L HD1 domain and…
- 5WXH 1.3 Å, Crystal structure of TAF3 PHD finger bound to H3K4me3
- 5FFV 1.3 Å, Crystal structure of the bromodomain of human BRPF1 in complex with H3K14ac histone…
- 4L7X 1.35 Å, Crystal structure of the DIDO PHD finger in complex with H3K4me3
- 6BHE 1.35 Å, Crystal structure of SETDB1 with a modified H3 peptide
- 6BHI 1.4 Å, Crystal structure of SETDB1 with a modified H3 peptide
- 3ASL 1.41 Å, Structure of UHRF1 in complex with histone tail
Browse structure collections
About this viewer
MolViewer shows 6UPL directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.