6UPV: Alpha-E-catenin ABD-F-actin complex

Alpha-E-catenin ABD-F-actin complex. Determined by electron microscopy at 3.2 Å resolution. Released 30 Sept 2020.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Homo sapiens, Gallus gallus
Chains: 7
Atoms: 17,233
Mol. weight: 412.05 kDa
Ligands: ADP, MG
Released: 30 Sept 2020

Explore 6UPV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6UPV contains 129 α-helices and 108 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-9	2	1
β-strand	11-12	2	2
β-strand	16-19	4	2
β-strand	21	1	1
β-strand	29-32	4	2
β-strand	35-38	4	3
β-strand	41-42	2	4
β-strand	53-54	2	3
α-helix	55-58	4
β-strand	65-68	4	3
β-strand	71-72	2	5
β-strand	75-76	2	5
α-helix	83-87	5
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	116-125	10
β-strand	131-136	6	1
α-helix	137-140	4
α-helix	143-145	3
β-strand	150-155	6	6
β-strand	160-166	7	6
β-strand	169-170	2	6
α-helix	172-174	3
β-strand	176-178	3	6
α-helix	183-192	10
α-helix	193-195	3
α-helix	206-213	8
α-helix	223-229	7
β-strand	238-241	4	7
β-strand	247-250	4	7
α-helix	258-261	4
α-helix	287-289	3
α-helix	290-295	6
β-strand	297-300	4	6
α-helix	303-305	3
α-helix	309-318	10
β-strand	329-330	2	6
α-helix	338-346	9
α-helix	352-354	3
β-strand	357-358	2	1
α-helix	361-364	4
α-helix	369-373	5

Chain B: 22 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-9	2	8
β-strand	11-12	2	9
β-strand	17-19	3	9
β-strand	21	1	8
β-strand	29-31	3	9
β-strand	35-38	4	10
β-strand	41-42	2	11
β-strand	53-54	2	10
α-helix	55-58	4
β-strand	65-68	4	10
β-strand	71-72	2	12
β-strand	75-76	2	12
α-helix	83-87	5
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	8
α-helix	116-125	10
β-strand	131-136	6	8
α-helix	137-143	7
β-strand	150-155	6	13
β-strand	160-166	7	13
β-strand	169-170	2	13
α-helix	172-174	3
β-strand	176-178	3	13
α-helix	183-192	10
α-helix	193-195	3
α-helix	206-213	8
α-helix	223-232	10
β-strand	238-241	4	14
β-strand	247-250	4	14
α-helix	258-261	4
α-helix	290-294	5
β-strand	297-300	4	13
α-helix	303-305	3
α-helix	309-317	9
β-strand	329-330	2	13
α-helix	335-337	3
α-helix	338-347	10
α-helix	352-354	3
β-strand	357-358	2	8
α-helix	361-364	4
α-helix	367-369	3
α-helix	370-373	4

Chain C: 20 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-9	2	15
β-strand	11-12	2	16
β-strand	17-19	3	16
β-strand	21	1	15
β-strand	29-31	3	16
β-strand	35-38	4	17
β-strand	41-42	2	18
β-strand	53-54	2	17
α-helix	57-60	4
β-strand	65-68	4	17
β-strand	71-72	2	19
β-strand	75-76	2	19
α-helix	82-87	6
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	15
α-helix	116-125	10
β-strand	131-136	6	15
α-helix	137-143	7
β-strand	150-155	6	4
β-strand	160-166	7	4
β-strand	169-170	2	4
α-helix	172-174	3
β-strand	176-178	3	4
α-helix	183-192	10
α-helix	193-195	3
α-helix	206-213	8
α-helix	223-232	10
β-strand	238-241	4	20
β-strand	247-250	4	20
α-helix	258-261	4
α-helix	287-289	3
α-helix	290-294	5
β-strand	297-300	4	4
α-helix	302-305	4
α-helix	309-317	9
β-strand	329-330	2	4
α-helix	338-347	10
β-strand	357-358	2	15
α-helix	361-364	4
α-helix	369-373	5

Chain D: 23 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-9	2	21
β-strand	11-12	2	22
β-strand	16-19	4	22
β-strand	21	1	21
β-strand	29-32	4	22
β-strand	35-38	4	23
β-strand	53-54	2	23
α-helix	57-60	4
β-strand	65-68	4	23
β-strand	71-72	2	24
β-strand	75-76	2	24
α-helix	79-87	9
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	21
α-helix	113-125	13
β-strand	131-136	6	21
α-helix	137-140	4
α-helix	143-145	3
β-strand	150-155	6	11
β-strand	160-166	7	11
β-strand	169-170	2	11
α-helix	172-174	3
β-strand	176-178	3	11
α-helix	183-192	10
α-helix	193-195	3
α-helix	208-213	6
α-helix	223-232	10
β-strand	238-241	4	25
β-strand	247-250	4	25
α-helix	258-261	4
α-helix	275-282	8
α-helix	287-289	3
α-helix	290-295	6
β-strand	297-300	4	11
α-helix	302-305	4
α-helix	310-317	8
β-strand	329-330	2	11
α-helix	335-337	3
α-helix	338-347	10
β-strand	357-358	2	21
α-helix	359-364	6
α-helix	369-373	5

Chain E: 21 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-9	2	26
β-strand	11	1	27
β-strand	16-19	4	27
β-strand	21	1	26
β-strand	29-32	4	27
β-strand	35-38	4	28
β-strand	53-54	2	28
α-helix	57-60	4
β-strand	65-68	4	28
β-strand	71-72	2	29
β-strand	75-76	2	29
α-helix	79-87	9
α-helix	88-92	5
α-helix	98-100	3
β-strand	103-107	5	26
α-helix	116-125	10
β-strand	131-136	6	26
α-helix	137-143	7
β-strand	150-155	6	18
β-strand	160-166	7	18
β-strand	169-170	2	18
α-helix	172-174	3
β-strand	176-178	3	18
α-helix	183-192	10
α-helix	193-195	3
α-helix	206-213	8
α-helix	223-232	10
β-strand	238-241	4	30
β-strand	247-250	4	30
α-helix	258-261	4
α-helix	264-267	4
α-helix	279-282	4
α-helix	290-295	6
β-strand	297-300	4	18
α-helix	303-305	3
α-helix	309-318	10
β-strand	329-330	2	18
α-helix	338-347	10
β-strand	357-358	2	26
α-helix	359-364	6
α-helix	369-373	5

Chain L: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	702-704	3
α-helix	711-727	17
α-helix	728-730	3
α-helix	739-752	14
α-helix	756-764	9
α-helix	774-780	7
α-helix	782-803	22
α-helix	816-826	11
α-helix	830-841	12
α-helix	850-852	3
α-helix	863-868	6

Chain M: 10 helices, 0 β-strands

Element	Residues	Length
α-helix	700-703	4
α-helix	713-727	15
α-helix	728-730	3
α-helix	739-766	28
α-helix	773-775	3
α-helix	777-804	28
α-helix	816-826	11
α-helix	835-841	7
α-helix	850-852	3
α-helix	863-868	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Catenin alpha-1	L, M	protein	906	Homo sapiens	P35221 (AlphaFold model)
Actin, alpha skeletal muscle	A, B, C, D, E	protein	375	Gallus gallus	P68139 (AlphaFold model)

Sequence of entity 1 (L, M), FASTA

>6UPV_1 Catenin alpha-1 (chains L, M)
MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHV
LAASVEQATENFLEKGDKIAKESQFLKEELVAAVEDVRKQGDLMKAAAGEFADDPCSSVK
RGNMVRAARALLSAVTRLLILADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKAL
KPEVDKLNIMAAKRQQELKDVGHRDQMAAARGILQKNVPILYTASQACLQHPDVAAYKAN
RDLIYKQLQQAVTGISNAAQATASDDASQHQGGGGGELAYALNNFDKQIIVDPLSFSEER
FRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMGNAGRKER
SDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYA
QVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLA
QENMDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDR
TAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSS
DPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTED
DQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMC
MIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYL
QRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVAS
TKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEF
KAMDSI

Sequence of entity 2 (A, B, C, D, E), FASTA

>6UPV_2 Actin, alpha skeletal muscle (chains A, B, C, D, E)
DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMT
QIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDL
AGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQ
EYDEAGPSIVHRKCF

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	5
MG	Magnesium ion	Mg	5

Primary citation

Molecular mechanism for direct actin force-sensing by alpha-catenin. Mei, L., Espinosa de Los Reyes, S., Reynolds, M.J. et al. Elife (2020) 9. DOI 10.7554/eLife.62514 · PubMed

Other PDB entries of the same protein (UniProt P35221 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6V2O 1.27 Å, HLA-B*57:01 presenting the peptide ASLNLPAVSW
6V2P 1.3 Å, HLA-B*57:03 presenting the peptide ASLNLPAVSW
9BL4 1.75 Å, KIR3DL1*086 in complex with HLA-B*57:03 presenting the AW10 peptide
9BL3 2.0 Å, KIR3DL1*114 in complex with HLA-B*57:03 presenting the AW10 peptide
9BL2 2.1 Å, KIR3DL1*001 in complex with HLA-B*57:03 presenting the AW10 peptide
1H6G 2.2 Å, alpha-catenin M-domain
4EHP 2.66 Å, Crystal Structure of human vinculin head domain (residues 1-252) in complex with…
7UTJ 2.77 Å, Cryogenic electron microscopy 3D map of F-actin bound by human dimeric alpha-catenin
4IGG 3.66 Å, Full-length human alpha-catenin crystal structure

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