6UUN: PDB entry 6UUN

CryoEM Structure of the active Adrenomedullin 1 receptor G protein complex with adrenomedullin peptide. Determined by electron microscopy at 3.0 Å resolution. Released 25 Mar 2020.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 8,980
Mol. weight: 187.37 kDa
Released: 25 Mar 2020

Explore 6UUN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6UUN contains 44 α-helices and 57 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	18-39	22
β-strand	40-46	7	1
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	235-238	4
β-strand	243-247	5	1
α-helix	265-278	14
β-strand	286-290	5	1
α-helix	313-317	5
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-362	4	1
α-helix	372-390	19

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	8-24	17
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	102-105	4	4
β-strand	111-115	5	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain E: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	61-76	16
α-helix	77-82	6
α-helix	90-105	16
α-helix	114-125	12
α-helix	140-142	3
α-helix	143-154	12
α-helix	157-166	10

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	16-21	6
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain N: 1 helix, 12 β-strands

Element	Residues	Length	Sheet
β-strand	5-7	3	9
β-strand	11	1	10
β-strand	17-23	7	9
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	58-60	3	11
α-helix	62-64	3
β-strand	68-73	6	9
β-strand	78-84	7	9
β-strand	92-98	7	11
β-strand	118	1	11
β-strand	122-124	3	11
β-strand	125	1	10

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	21-23	3
α-helix	24-33	10
β-strand	39	1	12
α-helix	44-47	4

Chain R: 18 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	36-54	19
β-strand	64-65	2	13
α-helix	66-67	2
β-strand	68-69	2	14
β-strand	74-75	2	14
β-strand	78-79	2	13
β-strand	82-87	6	15
α-helix	88-89	2
β-strand	92	1	12
β-strand	100-105	6	15
α-helix	111-112	2
β-strand	113-114	2	16
β-strand	119-120	2	16
β-strand	122-123	2	15
α-helix	125-166	42
α-helix	172-195	24
α-helix	202-207	6
α-helix	210-241	32
α-helix	254-256	3
α-helix	257-261	5
α-helix	264-278	15
α-helix	281-283	3
α-helix	291-293	3
α-helix	297-320	24
α-helix	334-345	12
α-helix	364-374	11
α-helix	376-384	9
α-helix	389-399	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Receptor activity-modifying protein 2	E	protein	156	Homo sapiens	O60895 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	138	Lama glama
ADM	P	protein	53	Homo sapiens	P35318
Calcitonin gene-related peptide type 1 receptor	R	protein	490	Homo sapiens	Q16602

Sequence of entity 1 (A), FASTA

>6UUN_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>6UUN_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (E), FASTA

>6UUN_3 Receptor activity-modifying protein 2 (chains E)
MKTIIALSYIFCLVFADYKDDDDKPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPI
EKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFS
DPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Sequence of entity 4 (G), FASTA

>6UUN_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>6UUN_5 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 6 (P), FASTA

>6UUN_6 ADM (chains P)
YRQSMNNFQGLRSFGCRFGTCTVQKLAHQIYQFTDKDKDNVAPRSKISPQGYX

Sequence of entity 7 (R), FASTA

>6UUN_7 Calcitonin gene-related peptide type 1 receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAELEESPEDSIQLGVTRNKIMTAQYECYQ
KIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGN
WFRHPASNRTWTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLS
CQRITLHKNLFFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWM
LCEGIYLHTLIVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHL
LYIIHGPICAALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFV
LIPWRPEGKIAEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSF
SNSEALRSASYTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYNPAGLEVLFQ
GPHHHHHHHH

Primary citation

Structure and Dynamics of Adrenomedullin Receptors AM1and AM2Reveal Key Mechanisms in the Control of Receptor Phenotype by Receptor Activity-Modifying Proteins. Liang, Y.L., Belousoff, M.J., Fletcher, M.M. et al. Acs Pharmacol Transl Sci (2020) 3:263-284. DOI 10.1021/acsptsci.9b00080 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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