6VTM: Human Cathepsin-G Inhibited by S. aureus EapH1

Human Cathepsin-G Inhibited by S. aureus EapH1. Determined by X-ray diffraction at 1.6 Å resolution. Released 22 Apr 2020.

Method: X-ray diffraction
Resolution: 1.6 Å
Organisms: Homo sapiens, Staphylococcus aureus (strain Mu50 / ATCC 700699)
Chains: 8
Atoms: 11,227
Mol. weight: 147.62 kDa
Released: 22 Apr 2020

Explore 6VTM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6VTM contains 46 α-helices and 128 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and C: 9 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	1
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-37	8	3
β-strand	40-50	11	3
β-strand	53-56	4	3
α-helix	58-60	3
β-strand	65-69	5	3
β-strand	73	1	4
β-strand	76	1	5
β-strand	82-91	10	3
β-strand	96	1	6
β-strand	101	1	6
β-strand	105-109	5	3
β-strand	116	1	7
β-strand	119	1	7
β-strand	123	1	2
α-helix	124-126	3
β-strand	136-141	6	2
β-strand	153	1	4
β-strand	155-161	7	2
α-helix	162-163	2
α-helix	164-170	7
β-strand	179-182	4	2
β-strand	190	1	1
β-strand	199-202	4	2
β-strand	205-213	9	2
α-helix	218	1
α-helix	220	1
β-strand	221-225	5	2
α-helix	226-229	4
α-helix	230-237	8

Chains B, E, F and H: 3 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	45-53	9	3
β-strand	56	1	3
β-strand	57-58	2	2
β-strand	61-66	6	3
β-strand	72-73	2	8
α-helix	74-89	16
α-helix	93-98	6
β-strand	102-108	7	3
β-strand	113-117	5	3
β-strand	127-128	2	8
α-helix	130-132	3
β-strand	133-140	8	3

Chain D: 6 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	15
β-strand	20-21	2	16
α-helix	22-23	2
β-strand	30-37	8	17
β-strand	40-50	11	17
β-strand	53-56	4	17
α-helix	58-60	3
β-strand	65-69	5	17
β-strand	73	1	18
β-strand	82-91	10	17
β-strand	96	1	19
β-strand	101	1	19
β-strand	105-109	5	17
β-strand	116	1	20
β-strand	119	1	20
β-strand	123	1	21
α-helix	124-126	3
β-strand	136-141	6	16
β-strand	146	1	22
β-strand	149	1	22
β-strand	153	1	18
β-strand	155-161	7	16
α-helix	164-170	7
β-strand	179-182	4	16
β-strand	190	1	15
β-strand	199-201	3	16
β-strand	205	1	21
β-strand	206-213	8	16
β-strand	221-225	5	16
α-helix	226-229	4
α-helix	230-237	8

Chain G: 10 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	25
β-strand	20-21	2	26
α-helix	22-23	2
β-strand	30-37	8	27
β-strand	40-50	11	27
β-strand	53-56	4	27
α-helix	58-60	3
β-strand	65-69	5	27
β-strand	73	1	28
β-strand	82-91	10	27
β-strand	96	1	29
β-strand	101	1	29
β-strand	105-109	5	27
β-strand	116	1	30
β-strand	119	1	30
α-helix	125-126	2
β-strand	136-141	6	26
β-strand	153	1	28
β-strand	155-161	7	26
α-helix	162-163	2
α-helix	164-170	7
β-strand	179-182	4	26
β-strand	190	1	25
α-helix	198	1
β-strand	199-202	4	26
β-strand	205-213	9	26
α-helix	218	1
α-helix	220	1
β-strand	221-225	5	26
α-helix	226-229	4
α-helix	230-237	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Cathepsin G	A, C, D, G	protein	224	Homo sapiens	P08311 (AlphaFold model)
MAP domain-containing protein	B, E, F, H	protein	99	Staphylococcus aureus (strain Mu50 / ATCC 700699)	A0A0H3K0M1 (AlphaFold model)

Sequence of entity 1 (A, C, D, G), FASTA

>6VTM_1 Cathepsin G (chains A, C, D, G)
IIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVLTAAHCWGSNINVTLGAHNIQ
RRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRRNRNVNPVALPRAQEGLRPGT
LCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAFKGD
SGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRTTMRS

Sequence of entity 2 (B, E, F, H), FASTA

>6VTM_2 MAP domain-containing protein (chains B, E, F, H)
GKHTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK

Primary citation

Crystal Structure of Human Cathepsin-G Inhibited by S. aureus EapH1. Herdendorf, T.J., Rooijakkers, S.H.M., Geisbrecht, B.V. J Biol Chem (2020).

Other PDB entries of the same protein (UniProt P08311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7H6G 1.21 Å, THE 1.21 A CRYSTAL STRUCTURE OF HUMAN CATHEPSIN G IN COMPLEX WITH…
1CGH 1.8 Å, Human cathepsin G
8G25 1.8 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
8G24 1.82 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
1T32 1.85 Å, A Dual Inhibitor of the Leukocyte Proteases Cathepsin G and Chymase with Therapeutic…
8D4V 1.85 Å, Crystal Structure of Cathepsin G Inhibited by Eap2 from S. aureus
8G26 1.85 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
1AU8 1.9 Å, Human cathepsin G
7H6H 1.94 Å, THE 1.94 A CRYSTAL STRUCTURE OF HUMAN CATHEPSIN G IN COMPLEX WITH…
8D4S 1.95 Å, Crystal Structure of Cathepsin G Inhibited by Eap1 from S. aureus
9ASX 1.96 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap3 of S. aureus
9ATK 2.11 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap4 of S. aureus

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