8G26: Cathepsin-G

Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at pH 8.5. Determined by X-ray diffraction at 1.85 Å resolution. Released 26 Apr 2023.

Method: X-ray diffraction
Resolution: 1.85 Å
Organisms: Homo sapiens, Staphylococcus aureus subsp. aureus Mu50
Chains: 3
Atoms: 4,496
Mol. weight: 62.49 kDa
Ligands: NAG
Released: 26 Apr 2023

Explore 8G26 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8G26 contains 16 α-helices and 50 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	1
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-37	8	3
β-strand	40-50	11	3
β-strand	53-56	4	3
α-helix	58-60	3
β-strand	64-69	6	3
β-strand	73	1	4
β-strand	82-91	10	3
β-strand	96	1	5
β-strand	101	1	5
β-strand	105-109	5	3
α-helix	123-126	4
β-strand	136-141	6	2
β-strand	153	1	4
β-strand	155-161	7	2
α-helix	165-168	4
β-strand	179-182	4	2
β-strand	190	1	1
β-strand	199-202	4	2
β-strand	205-213	9	2
α-helix	218	1
α-helix	220	1
β-strand	221-225	5	2
α-helix	226-229	4
α-helix	230-237	8

Chain B: 5 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	30	1	6
β-strand	33-34	2	7
α-helix	35-36	2
β-strand	43-48	6	8
β-strand	51-60	10	8
β-strand	63-66	4	8
α-helix	68-70	3
α-helix	76-78	3
β-strand	80-83	4	8
β-strand	87	1	9
β-strand	96-105	10	8
β-strand	109	1	10
β-strand	114	1	10
β-strand	118-122	5	8
β-strand	149-154	6	7
β-strand	157	1	11
β-strand	164	1	11
β-strand	167	1	9
β-strand	169-176	8	7
β-strand	185-188	4	7
β-strand	195	1	6
β-strand	204	1	7
β-strand	213-219	7	7
β-strand	229-233	5	7
α-helix	234-236	3
α-helix	238-245	8

Chain C: 3 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	45-54	10	3
β-strand	57	1	3
β-strand	58-59	2	2
β-strand	62-68	7	3
β-strand	72-73	2	12
α-helix	75-90	16
α-helix	94-99	6
β-strand	102-109	8	3
β-strand	114-118	5	3
β-strand	123-125	3	7
β-strand	129-130	2	12
α-helix	131-133	3
β-strand	134-142	9	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Cathepsin-G	A	protein	223	Homo sapiens	P08311 (AlphaFold model)
Neutrophil elastase	B	protein	218	Homo sapiens	P08246 (AlphaFold model)
MAP domain-containing protein	C	protein	117	Staphylococcus aureus subsp. aureus Mu50	A0A0H3JUK5 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8G26_1 Cathepsin-G (chains A)
IIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVLTAAHCWGSNINVTLGAHNIQ
RRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRRNRNVNPVALPRAQEGLRPGT
LCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAFKGD
SGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRTTMR

Sequence of entity 2 (B), FASTA

>8G26_2 Neutrophil elastase (chains B)
IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNL
SRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGV
QCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCN
GLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQ

Sequence of entity 3 (C), FASTA

>8G26_3 MAP domain-containing protein (chains C)
GSTAEKDKLPATQKAKEMQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLY
DERGVTPEKIRNAKSAVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1

Primary citation

Simultaneous inhibition of two neutrophil serine proteases by the S. aureus innate immune evasion protein EapH2. Mishra, N., Herdendorf, T.J., Prakash, O. et al. J Biol Chem (2023) 299:104878-104878. DOI 10.1016/j.jbc.2023.104878 · PubMed

Other PDB entries of the same protein (UniProt P08311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7H6G 1.21 Å, THE 1.21 A CRYSTAL STRUCTURE OF HUMAN CATHEPSIN G IN COMPLEX WITH…
6VTM 1.6 Å, Human Cathepsin-G Inhibited by S. aureus EapH1
1CGH 1.8 Å, Human cathepsin G
8G25 1.8 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
8G24 1.82 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
1T32 1.85 Å, A Dual Inhibitor of the Leukocyte Proteases Cathepsin G and Chymase with Therapeutic…
8D4V 1.85 Å, Crystal Structure of Cathepsin G Inhibited by Eap2 from S. aureus
1AU8 1.9 Å, Human cathepsin G
7H6H 1.94 Å, THE 1.94 A CRYSTAL STRUCTURE OF HUMAN CATHEPSIN G IN COMPLEX WITH…
8D4S 1.95 Å, Crystal Structure of Cathepsin G Inhibited by Eap1 from S. aureus
9ASX 1.96 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap3 of S. aureus
9ATK 2.11 Å, BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap4 of S. aureus

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