CryoEM structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer, in complex with histamine and megabody Mb25 in lipid nanodisc. Determined by electron microscopy at 1.7 Å resolution. Released 18 Nov 2020.
Explore 7A5V in 3D Show helices and sheets RCSB PDB PDBe
7A5V contains 16 α-helices and 24 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 9-20 | 12 | |
| α-helix | 35 | 1 | |
| β-strand | 36-51 | 16 | 1 |
| β-strand | 56-68 | 13 | 1 |
| α-helix | 70-72 | 3 | |
| β-strand | 81-83 | 3 | 1 |
| α-helix | 85-90 | 6 | |
| β-strand | 96-98 | 3 | 2 |
| β-strand | 101-106 | 6 | 1 |
| β-strand | 114-118 | 5 | 1 |
| β-strand | 123-135 | 13 | 1 |
| β-strand | 147-156 | 10 | 2 |
| β-strand | 164-168 | 5 | 1 |
| α-helix | 171-173 | 3 | |
| β-strand | 175-176 | 2 | 1 |
| α-helix | 178-180 | 3 | |
| β-strand | 183 | 1 | 2 |
| β-strand | 186-200 | 15 | 2 |
| β-strand | 203-216 | 14 | 2 |
| α-helix | 219-221 | 3 | |
| α-helix | 222-226 | 5 | |
| α-helix | 227-237 | 11 | |
| α-helix | 238-241 | 4 | |
| α-helix | 247-269 | 23 | |
| α-helix | 280-305 | 26 | |
| α-helix | 310-446 | 30 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-7 | 6 | 3 |
| β-strand | 10-11 | 2 | 4 |
| β-strand | 405-413 | 9 | 3 |
| β-strand | 420-426 | 7 | 4 |
| α-helix | 431-432 | 2 | |
| β-strand | 433-439 | 7 | 4 |
| β-strand | 445-447 | 3 | 4 |
| α-helix | 449-451 | 3 | |
| β-strand | 455-460 | 6 | 3 |
| β-strand | 465-470 | 6 | 3 |
| β-strand | 479-486 | 8 | 4 |
| α-helix | 496-498 | 3 | |
| β-strand | 499-502 | 4 | 4 |
| β-strand | 506-509 | 4 | 4 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3 | A | protein | 396 | Homo sapiens | P28472 (AlphaFold model) |
| Megabody Mb25 | O | protein | 522 | Lama glama |
>7A5V_1 Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3 (chains A) EMDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPDYDIPTTENLYFQGTGQSVND PGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQ YWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVL YGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQ FSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYD ASAARVALGITTVLTMTTINTHLRETLPKIPYVTAIDMYLMGCFVFVFLALLEYAFVNYI FFSQPARAAAIDRWSRIVFPFTFSLFNLVYWLYYVN
>7A5V_2 Megabody Mb25 (chains O) QVQLVESGGGLVQTKTTTSVIDTTNDAQNLLTQAQTIVNTLKDYCPILIAKSSSSNGGTN NANTPSWQTAGGGKNSCATFGAEFSAASDMINNAQKIVQETQQLSANQPKNITQPHNLNL NSPSSLTALAQKMLKNAQSQAEILKLANQVESDFNKLSSGHLKDYIGKCDASAISSANMT MQNQKNNWGNGCAGVEETQSLLKTSAADFNNQTPQINQAQNLANTLIQELGNNTYEQLSR LLTNDNGTNSKTSAQAINQAVNNLNERAKTLAGGTTNSPAYQATLLALRSVLGLWNSMGY AVICGGYTKSPGENNQKDFHYTDENGNGTTINCGGSTNSNGTHSYNGTNTLKADKNVSLS IEQYEKIHEAYQILSKALKQAGLAPLNSKGEKLEAHVTTSKYGSLRLSCAASGHTFNYPI MGWFRQAPGKEREFVGAISWSGGSTSYADSVKDRFTISRDNAKNTVYLEMNNLKPEDTAV YYCAAKGRYSGGLYYPTNYDYWGQGTQVTVSSHHHHHHEPEA
| ID | Name | Formula | Copies |
|---|---|---|---|
| HSM | Histamine | C5 H9 N3 | 1 |
| OCT | N-octane | C8 H18 | 1 |
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 1 |
| D10 | Decane | C10 H22 | 4 |
| R16 | Hexadecane | C16 H34 | 1 |
Water and common crystallization additives (CL) are not listed.
Single-particle cryo-EM at atomic resolution. Nakane, T., Kotecha, A., Sente, A. et al. Nature (2020) 587:152-156. DOI 10.1038/s41586-020-2829-0 · PubMed
Other PDB entries of the same protein (UniProt P28472 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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