7AEW: 14-3-3 sigma

14-3-3 sigma bound to bis-phosphorylated aminopeptidase N (APN, CD13) via canonical and non-canonical binding motifs. Determined by X-ray diffraction at 1.2 Å resolution. Released 4 Nov 2020.

Method: X-ray diffraction
Resolution: 1.2 Å
Organism: Homo sapiens
Chains: 3
Atoms: 2,636
Mol. weight: 34.81 kDa
Released: 4 Nov 2020

Explore 7AEW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7AEW contains 15 α-helices and 2 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain AAA: 15 helices, 0 β-strands

Element	Residues	Length
α-helix	3-15	13
α-helix	19-30	12
α-helix	35-37	3
α-helix	38-69	32
α-helix	74-76	3
α-helix	80-102	23
α-helix	103-107	5
α-helix	108-110	3
α-helix	114-134	21
α-helix	140-161	22
α-helix	167-178	12
α-helix	179-183	5
α-helix	187-204	18
α-helix	205-207	3
α-helix	210-230	21

Chain BBB: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	61-64	4	1

Chain CCC: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	41-45	5	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
14-3-3 protein sigma	AAA	protein	236	Homo sapiens	P31947 (AlphaFold model)
Aminopeptidase N	BBB, CCC	protein	38	Homo sapiens	P15144 (AlphaFold model)

Sequence of entity 1 (AAA), FASTA

>7AEW_1 14-3-3 protein sigma (chains AAA)
GAMGSMERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQ
RAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAE
SRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALN
FSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWT

Sequence of entity 2 (BBB, CCC), FASTA

>7AEW_2 Aminopeptidase N (chains BBB, CCC)
EKNKNANSSPVASTTPSASATTNPASATTLDQSKAWNR

Primary citation

MMP activation-associated aminopeptidase N reveals a bivalent 14-3-3 binding motif. Kiehstaller, S., Ottmann, C., Hennig, S. J Biol Chem (2020) 295:18266-18275. DOI 10.1074/jbc.RA120.014708 · PubMed

Other PDB entries of the same protein (UniProt P31947 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3IQU 1.05 Å, Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (6mer)
7AZ2 1.08 Å, 14-3-3 sigma with Pin1 binding site pS72 and covalently bound LvD1014
6HMT 1.1 Å, Ternary complex of Estrogen Receptor alpha peptide and 14-3-3 sigma C42 mutant bound to…
7BAA 1.1 Å, Cys-42-tethered stabilizer 12 of 14-3-3(sigma)/ERa PPI
8BZC 1.1 Å, co-soaked stabilizers for ERa - 14-3-3 interaction (884_AZ244)
8BZW 1.1 Å, Co-soaked stabilizers for ERa - 14-3-3 interaction (844_AZ210)
8C04 1.1 Å, Co-soaked stabilizers for ERa - 14-3-3 interaction (884_AZ354)
9GG8 1.1 Å, Crystal structure of 14-3-3 sigma dC - C38N in complex with Tau pS214 peptide and…
8ANC 1.11 Å, 14-3-3 sigma sirtuin-3 phospho-peptide complex
6Y1J 1.13 Å, 14-3-3 sigma in complex with IkappaBalpha pS63 peptide
6G6X 1.13 Å, 14-3-3sigma in complex with a P129beta3P mutated YAP pS127 phosphopeptide
6NV2 1.13 Å, 14-3-3 sigma with RelA/p65 binding site pS45 in complex with DP005

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