Bacillus subtilis ribosome-associated quality control complex state B, multibody refinement focussed on RqcH. Ribosomal 50S subunit with P-tRNA, RqcH, and RqcP/YabO. Determined by electron microscopy at 3.5 Å resolution. Released 23 Dec 2020.
Explore 7ASA in 3D Show helices and sheets RCSB PDB PDBe
7ASA contains 28 α-helices and 40 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 5-19 | 15 | |
| β-strand | 23-31 | 9 | 1 |
| β-strand | 34-41 | 8 | 1 |
| β-strand | 44-51 | 8 | 1 |
| β-strand | 58-62 | 5 | 1 |
| α-helix | 74-82 | 9 | |
| β-strand | 87-92 | 6 | 1 |
| β-strand | 99-107 | 9 | 1 |
| β-strand | 113-121 | 9 | 1 |
| α-helix | 124-126 | 3 | |
| β-strand | 128-133 | 6 | 1 |
| β-strand | 138 | 1 | 2 |
| β-strand | 139-142 | 4 | 1 |
| β-strand | 146 | 1 | 3 |
| α-helix | 148-150 | 3 | |
| β-strand | 156 | 1 | 3 |
| β-strand | 161 | 1 | 2 |
| α-helix | 162 | 1 | |
| α-helix | 164-167 | 4 | |
| β-strand | 171 | 1 | 4 |
| α-helix | 180-184 | 5 | |
| α-helix | 193-199 | 7 | |
| β-strand | 200 | 1 | 4 |
| α-helix | 205-213 | 9 | |
| α-helix | 224-235 | 12 | |
| β-strand | 241-246 | 6 | 5 |
| β-strand | 249-254 | 6 | 5 |
| β-strand | 263-266 | 4 | 5 |
| α-helix | 270-278 | 9 | |
| α-helix | 281-316 | 36 | |
| α-helix | 322-335 | 14 | |
| β-strand | 345-349 | 5 | 6 |
| β-strand | 358-362 | 5 | 6 |
| α-helix | 369-413 | 45 | |
| α-helix | 416-428 | 13 | |
| β-strand | 451-454 | 4 | 7 |
| β-strand | 460-463 | 4 | 7 |
| α-helix | 467-474 | 8 | |
| β-strand | 483-487 | 5 | 7 |
| β-strand | 492 | 1 | 8 |
| β-strand | 495-497 | 3 | 7 |
| α-helix | 504-515 | 12 | |
| β-strand | 518 | 1 | 8 |
| β-strand | 528-532 | 5 | 7 |
| β-strand | 552-555 | 4 | 7 |
| α-helix | 562-564 | 3 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2 | 1 | 9 |
| α-helix | 3-9 | 7 | |
| α-helix | 16-24 | 9 | |
| β-strand | 28-30 | 3 | 10 |
| β-strand | 33-34 | 2 | 10 |
| β-strand | 40 | 1 | 9 |
| β-strand | 46-51 | 6 | 10 |
| β-strand | 54-60 | 7 | 10 |
| α-helix | 69-74 | 6 | |
| β-strand | 76-82 | 7 | 10 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 11-14 | 4 | 11 |
| α-helix | 21-31 | 11 | |
| α-helix | 35-45 | 11 | |
| β-strand | 53-60 | 8 | 11 |
| β-strand | 66-70 | 5 | 11 |
| α-helix | 71-74 | 4 | |
| α-helix | 75-83 | 9 | |
| β-strand | 98-100 | 3 | 12 |
| α-helix | 102-112 | 11 | |
| α-helix | 113-115 | 3 | |
| α-helix | 121-133 | 13 | |
| β-strand | 137-139 | 3 | 12 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Rqc2 homolog RqcH | 0 | protein | 597 | Bacillus subtilis (strain 168) | O34693 (AlphaFold model) |
| Uncharacterized protein YabO | 1 | protein | 86 | Bacillus subtilis (strain 168) | P37557 (AlphaFold model) |
| tRNA-Ala-1-1 | 2 | RNA | 76 | Bacillus subtilis subsp. subtilis str. 168 | |
| 23S rRNA | A | RNA | 2926 | Bacillus subtilis subsp. subtilis str. 168 | |
| 50S ribosomal protein L11 | K | protein | 141 | Bacillus subtilis (strain 168) | Q06796 (AlphaFold model) |
>7ASA_1 Rqc2 homolog RqcH (chains 0) MSFDGMFTYGMTHELNEKIMGGRITKIHQPYKHDVIFHIRAKGKNQKLLLSAHPSYSRVH ITAQAYENPSEPPMFCMLLRKHIEGGFIEKIEQAGLDRIMIFHIKSRNEIGDETVRKLYV EIMGRHSNIILTDAAENVIIDGLKHLSPSMNSYRTVLPGQDYKLPPAQDKISPLEASEDD ILRHLSFQEGRLDKQIVDHFSGVSPLFAKEAVHRAGLANKVTLPKALLALFAEVKEHRFI PNITTVNGKEYFYLLELTHLKGEARRFDSLSELLDRFYFGKAERDRVKQQAQDLERFVVN ERKKNANKIKKLEKTLEYSENAKEFQLYGELLTANLYMLKKGDKQAEVINYYDEESPTIT IPLNPNKTPSENAQAYFTKYQKAKNSVAVVEEQIRLAQEEIEYFDQLIQQLSSASPRDIS EIREELVEGKYLRPKQQKGQKKQKPHNPVLETYESTSGLTILVGKNNRQNEYLTTRVAAR DDIWLHTKDIPGSHVVIRSSEPDEQTIMEAATIAAYFSKAKDSSSVPVDYTKIRHVKKPN GAKPGFVTYDSQHTVFVTPDADTVIKLKKSGSGGDYKDHDGDYKDHDIDYKDDDDKG
>7ASA_2 Uncharacterized protein YabO (chains 1) MRLDKFLKVSRLIKRRTLAKEVADQGRISINGNQAKASSDVKPGDELTVRFGQKLVTVQV NELKDTTKKEEAANMYTILKEEKLGE
>7ASA_3 tRNA-Ala-1-1 (chains 2) GGGGCCUUAGCUCAGCUGGGAGAGCGCCUGCUUUGCACGCAGGAGGUCAGCGGUUCGAUC CCGCUAGGCUCCACCA
>7ASA_4 23S rRNA (chains A) GGUUAAGUUAGAAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGG ACGAACACCGAUAUGCUUCGGGGAGCUGUAAGCAAGCUUUGAUCCGGAGAUUUCCGAAUG GGGAAACCCACCACUCGUAAUGGAGUGGUAUCCAUAUCUGAAUUCAUAGGAUAUGAGAAG GCAGACCCGGGGAACUGAAACAUCUAAGUACCCGGAGGAAGAGAAAGCAAAUGCGAUUCC CUGAGUAGCGGCGAGCGAAACGGGAUCAGCCCAAACCAAGAGGCUUGCCUCUUGGGGUUG UAGGACACUCUGUACGGAGUUACAAAGGAACGAGGUAGAUGAAGAGGUCUGGAAAGGCCC GCCAUAGGAGGUAACAGCCCUGUAGUCAAAACUUCGUUCUCUCCUGAGUGGAUCCUGAGU ACGGCGGAACACGUGAAAUUCCGUCGGAAUCCGGGAGGACCAUCUCCCAAGGCUAAAUAC UCCCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGG GGAGUGAAAGAGAUCCUGAAACCGUGUGCCUACAAGUAGUCAGAGCCCGUUAACGGGUGA UGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUCUCGUGCAAGGUUAAGCAGAAG AUGCGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGCAUGAGUACGUGGUCGUAGACCC GAAACCAGGUGAUCUACCCAUGUCCAGGGUGAAGUUCAGGUAACACUGAAUGGAGGCCCG AACCCACGCACGUUGAAAAGUGCGGGGAUGAGGUGUGGGUAGGGGUGAAAUGCCAAUCGA ACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGGUAAGAGUCU UGGAGGUAGAGCACUGAUUGGACUAGGGGCCCCUACCGGGUUACCGAAUUCAGUCAAACU CCGAAUGCCAAUGACUUAUCCUUGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAA AGGGAAACAGCCCAGACCGCCAGCUAAGGUCCCAAAGUAUACGUUAAGUGGAAAAGGAUG UGGAGUUGCUUAGACAACCAGGAUGUUGGCUUAGAAGCAGCCACCAUUUAAAGAGUGCGU AAUAGCUCACUGGUCGAGUGACUCUGCGCCGAAAAUGUACCGGGGCUAAACGUAUCACCG AAGCUGCGGACUGUUCUUCGAACAGUGGUAGGAGAGCGUUCUAAGGGCUGUGAAGCCAGA CCGGAAGGACUGGUGGAGCGCUUAGAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGAGGGG UGAGAAUCCCCUCCACCGAAUGCCUAAGGUUUCCUGAGGAAGGCUCGUCCGCUCAGGGUU AGUCGGGACCUAAGCCGAGGCCGAAAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCCUG UACCACCUCCUCACCAUUUGAGCAAUGGGGGGACGCAGGAGGAUAGGGUAAGCGCGGUAU UGGAUAUCCGCGUCCAAGCAGUUAGGCUGGGAAAUAGGCAAAUCCGUUUCCCAUAAGGCU GAGCUGUGAUGGCGAGCGAAAUAUAGUAGCGAAGUUCCUGAUUCCACACUGCCAAGAAAA GCCUCUAGCGAGGUGAGAGGUGCCCGUACCGCAAACCGACACAGGUAGGCGAGGAGAGAA UCCUAAGGUGAUCGAGAGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCG GGAGAAGGGGUGCUCUGUUAGGGUGCAAGCCCGAGAGAGCCGCAGUGAAUAGGCCCAGGC GACUGUUUAGCAAAAACACAGGUCUCUGCGAAGCCGUAAGGCGAAGUAUAGGGGCUGACG CCUGCCCGGUGCUGGAAGGUUAAGAGGAGCGCUUAGCGUAAGCGAAGGUGCGAAUUGAAG CCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGU AAGUUCCGACCCGCACGAAAGGCGCAACGAUCUGGGCACUGUCUCAACGAGAGACUCGGU GAAAUUAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGA GCUUUACUGCAGCCUGAUAUUGAAUGUUGGUACAGCUUGUACAGGAUAGGUAGGAGCCUU GGAAACCGGAGCGCCAGCUUCGGUGGAGGCAUCGGUGGGAUACUACCCUGGCUGUAUUGA CCUUCUAACCCGCCGCCCUUAUCGGGCGGGGAGACAGUGUCAGGUGGGCAGUUUGACUGG GGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGUUCCCUCAGAAUGGUUGGAAA UCAUUCGCAGAGUGUAAAGGCACAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGG GACGAAAGUCGGGCUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAU AAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUCCACAUCGACGGGGAGGU UUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGC UGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCC CUAUCCGUCGCGGGCGCAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGA UGGACGCACCGCUGGUGUACCAGUUGUUCUGCCAAGGGCAUCGCUGGGUAGCUAUGUGCG GACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCCCAU UCCGCAAGGAAGUAAGAUCCCUGAAAGAUGAUCAGGUUGAUAGGUCUGAGGUGGAAGUGU GGCGACACAUGGAGCUGACAGAUACUAAUCGAUCGAGGACUUAACC
>7ASA_5 50S ribosomal protein L11 (chains K) MAKKVVKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNARTADQAGLIIPVEISV YEDRSFTFITKTPPAAVLLKKAAGIESGSGEPNRNKVATVKRDKVREIAETKMPDLNAAD VEAAMRMVEGTARSMGIVIED
Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP. Crowe-McAuliffe, C., Takada, H., Murina, V. et al. Mol Cell (2021) 81:115. DOI 10.1016/j.molcel.2020.11.002 · PubMed
Other PDB entries of the same protein (UniProt O34693 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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