7B1G: TRPC4
TRPC4 in complex with Calmodulin. Determined by electron microscopy at 3.6 Å resolution. Released 9 Dec 2020.
- Method
- Electron microscopy
- Resolution
- 3.6 Å
- Organisms
- Danio rerio, Mus musculus
- Chains
- 5
- Atoms
- 21,661
- Mol. weight
- 439.3 kDa
- Ligands
- CA, 44E
- Released
- 9 Dec 2020
Explore 7B1G in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7B1G contains 159 α-helices and 10 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 40 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 33-41 | 9 | |
| α-helix | 46-57 | 12 | |
| α-helix | 73-79 | 7 | |
| α-helix | 83-91 | 9 | |
| α-helix | 99-105 | 7 | |
| α-helix | 109-116 | 8 | |
| α-helix | 145-152 | 8 | |
| α-helix | 155-163 | 9 | |
| α-helix | 190-203 | 14 | |
| α-helix | 206-211 | 6 | |
| α-helix | 216-234 | 19 | |
| α-helix | 238-257 | 20 | |
| α-helix | 262-269 | 8 | |
| α-helix | 287-294 | 8 | |
| α-helix | 304-314 | 11 | |
| α-helix | 328-349 | 22 | |
| α-helix | 356-360 | 5 | |
| α-helix | 362-382 | 21 | |
| α-helix | 397-400 | 4 | |
| α-helix | 401-424 | 24 | |
| α-helix | 433-457 | 25 | |
| α-helix | 465-467 | 3 | |
| α-helix | 468-469 | 2 | |
| α-helix | 473-491 | 19 | |
| α-helix | 492-497 | 6 | |
| α-helix | 502-510 | 9 | |
| α-helix | 513-537 | 25 | |
| β-strand | 551 | 1 | 1 |
| β-strand | 558 | 1 | 1 |
| α-helix | 564-573 | 10 | |
| α-helix | 574-576 | 3 | |
| α-helix | 591-606 | 16 | |
| α-helix | 607-612 | 6 | |
| α-helix | 613-625 | 13 | |
| α-helix | 631-647 | 17 | |
| α-helix | 655-657 | 3 | |
| α-helix | 661-662 | 2 | |
| α-helix | 669-673 | 5 | |
| α-helix | 678-680 | 3 | |
| α-helix | 695-720 | 26 | |
| α-helix | 721-725 | 5 | |
| β-strand | 731 | 1 | 2 |
| α-helix | 732-751 | 20 | |
Chain B: 38 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-42 | 11 | |
| α-helix | 45-57 | 13 | |
| β-strand | 66 | 1 | 3 |
| β-strand | 72 | 1 | 3 |
| α-helix | 73-80 | 8 | |
| α-helix | 83-90 | 8 | |
| α-helix | 99-105 | 7 | |
| α-helix | 109-116 | 8 | |
| α-helix | 145-152 | 8 | |
| α-helix | 155-163 | 9 | |
| α-helix | 193-202 | 10 | |
| α-helix | 206-211 | 6 | |
| α-helix | 218-234 | 17 | |
| α-helix | 238-245 | 8 | |
| α-helix | 247-256 | 10 | |
| α-helix | 262-269 | 8 | |
| α-helix | 289-293 | 5 | |
| α-helix | 306-314 | 9 | |
| α-helix | 328-338 | 11 | |
| α-helix | 340-349 | 10 | |
| α-helix | 362-381 | 20 | |
| α-helix | 382-384 | 3 | |
| α-helix | 401-424 | 24 | |
| α-helix | 433-456 | 24 | |
| α-helix | 465-467 | 3 | |
| α-helix | 468-469 | 2 | |
| α-helix | 473-488 | 16 | |
| α-helix | 489-497 | 9 | |
| α-helix | 502-511 | 10 | |
| α-helix | 513-519 | 7 | |
| α-helix | 522-538 | 17 | |
| β-strand | 551 | 1 | 4 |
| β-strand | 558 | 1 | 4 |
| α-helix | 564-573 | 10 | |
| α-helix | 591-600 | 10 | |
| α-helix | 602-607 | 6 | |
| α-helix | 612-626 | 15 | |
| α-helix | 631-643 | 13 | |
| α-helix | 644-646 | 3 | |
| α-helix | 655-658 | 4 | |
| α-helix | 697-721 | 25 | |
| β-strand | 729 | 1 | 2 |
| α-helix | 732-752 | 21 | |
Chain C: 39 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-42 | 11 | |
| α-helix | 45-57 | 13 | |
| α-helix | 73-79 | 7 | |
| α-helix | 83-91 | 9 | |
| α-helix | 99-105 | 7 | |
| α-helix | 109-116 | 8 | |
| α-helix | 145-152 | 8 | |
| α-helix | 155-163 | 9 | |
| α-helix | 190-203 | 14 | |
| α-helix | 206-211 | 6 | |
| α-helix | 216-234 | 19 | |
| α-helix | 238-256 | 19 | |
| α-helix | 262-269 | 8 | |
| α-helix | 289-292 | 4 | |
| α-helix | 298-301 | 4 | |
| α-helix | 304-314 | 11 | |
| α-helix | 328-337 | 10 | |
| α-helix | 340-349 | 10 | |
| α-helix | 356-359 | 4 | |
| α-helix | 362-383 | 22 | |
| α-helix | 397-400 | 4 | |
| α-helix | 401-422 | 22 | |
| α-helix | 435-456 | 22 | |
| α-helix | 465-467 | 3 | |
| α-helix | 468-469 | 2 | |
| α-helix | 473-489 | 17 | |
| α-helix | 490-497 | 8 | |
| α-helix | 503-510 | 8 | |
| α-helix | 514-517 | 4 | |
| α-helix | 520-538 | 19 | |
| α-helix | 564-574 | 11 | |
| α-helix | 591-607 | 17 | |
| α-helix | 608-612 | 5 | |
| α-helix | 613-626 | 14 | |
| α-helix | 633-647 | 15 | |
| α-helix | 655-657 | 3 | |
| α-helix | 699-717 | 19 | |
| α-helix | 721-725 | 5 | |
| α-helix | 732-749 | 18 | |
Chain D: 39 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-41 | 10 | |
| α-helix | 45-54 | 10 | |
| β-strand | 66 | 1 | 5 |
| β-strand | 72 | 1 | 5 |
| α-helix | 73-79 | 7 | |
| α-helix | 83-91 | 9 | |
| α-helix | 99-105 | 7 | |
| α-helix | 109-116 | 8 | |
| α-helix | 145-152 | 8 | |
| α-helix | 155-162 | 8 | |
| α-helix | 168-170 | 3 | |
| α-helix | 191-200 | 10 | |
| α-helix | 206-211 | 6 | |
| α-helix | 216-234 | 19 | |
| α-helix | 242-256 | 15 | |
| α-helix | 262-269 | 8 | |
| α-helix | 287-294 | 8 | |
| α-helix | 308-314 | 7 | |
| α-helix | 326-337 | 12 | |
| α-helix | 340-349 | 10 | |
| α-helix | 356-359 | 4 | |
| α-helix | 362-383 | 22 | |
| α-helix | 397-399 | 3 | |
| α-helix | 401-424 | 24 | |
| α-helix | 433-456 | 24 | |
| α-helix | 465-467 | 3 | |
| α-helix | 468-469 | 2 | |
| α-helix | 473-489 | 17 | |
| α-helix | 491-497 | 7 | |
| α-helix | 502-508 | 7 | |
| α-helix | 514-517 | 4 | |
| α-helix | 523-538 | 16 | |
| α-helix | 564-567 | 4 | |
| α-helix | 591-607 | 17 | |
| α-helix | 608-612 | 5 | |
| α-helix | 613-626 | 14 | |
| α-helix | 633-647 | 15 | |
| α-helix | 655-658 | 4 | |
| α-helix | 699-720 | 22 | |
| α-helix | 721-725 | 5 | |
| α-helix | 732-752 | 21 | |
Chain E: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 109-112 | 4 | |
| α-helix | 119-122 | 4 | |
| α-helix | 123-125 | 3 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Transient receptor potential cation channel subfamily c member 4a | A, B, C, D | protein | 915 | Danio rerio | U3N7D8 (AlphaFold model) |
| Calmodulin-1 | E | protein | 149 | Mus musculus | P0DP26 (AlphaFold model) |
Sequence of entity 1 (A, B, C, D), FASTA
>7B1G_1 Transient receptor potential cation channel subfamily c member 4a (chains A, B, C, D)
GSQLYFRRTDNSSYRDRIPLRIVRAESELSTQEKSYLSAVEKGDYASVKLALEEAEIYFK
ININCIDPLGRTALLIAIENENLEIIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKK
PSGEKQVPPILLDKQFSDFTPDITPIILAAHTNNYEIIKMLVQKGVSVPQPHEVRCNCVE
CVSSSDVDSLRHSRSRLNIYKALASPSLIALSSEDPFLTAFQLSWELQELSKVENEFKAE
YEELSHQCKHFAKDLLDQTRSSRELELILNFRDDMNLLQDEANNELARLKLAIKYRQKEF
VAQPNCQQLLASRWYDEFPGWRRRHWAGKLITCVFIGLMFPLLSLCYLVAPKSRYGLFIR
KPFIKFICHTASYLTFLFLLLLASQHIVSNNPDRQGPKPTTVEWMILPWVLGFIWTEIKQ
MWDGGFQDYIHDWWNLMDFVMNSLYLATISLKIVAYVKYSGCKPRDTWEMWHPTLVAEAV
FAIANIFSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFY
YENSEGMTCKGIRCERQNNAFSTLFETLQSLFWSIFGLISLYVTNVKADHKFTEFVGATM
FGTYNVISLVVLLNMLIAMMNNSYQHIADHADIEWKFARTKLWMSYFEEGGTLPPPFNII
PSPKSICYLITWIKVHVFKRRSKRTETFGTLGRRAAENVRLNHQYQEVLRNLVKRYVAAM
IRDAKTEEGLTEENFKELKQDISSFRYEVIGMMKGNRKSTRANKSDTSASDVSHPEGSLQ
YSSALKQNSKLHLYDVTTALQQQNSEEAKASLGCLANGSAVVLTEPILKDKARSDFPKDF
TDFGLFPKKQNPNKIYSLAEEATESDPDILDWGKEDKPLAGKVEQDVNESKCLMEEDERV
LEEQEMEHIASSHEH
Sequence of entity 2 (E), FASTA
>7B1G_2 Calmodulin-1 (chains E)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 4 |
| 44E | (2R)-3-(phosphonooxy)propane-1,2-diyl dihexanoate | C15 H29 O8 P | 4 |
Primary citation
Structural basis of TRPC4 regulation by calmodulin and pharmacological agents. Vinayagam, D., Quentin, D., Yu-Strzelczyk, J. et al. Elife (2020) 9. DOI 10.7554/eLife.60603 · PubMed
Other PDB entries of the same protein (UniProt U3N7D8 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7B0J 2.85 Å, TRPC4 in LMNG detergent
- 9FXL 3.0 Å, TRPC4 in complex with E-AzPico
- 9FXM 3.1 Å, TRPC4 in complex with Z-AzPico
- 7B16 3.15 Å, TRPC4 in complex with inhibitor GFB-9289
- 6G1K 3.6 Å, Electron cryo-microscopy structure of the canonical TRPC4 ion channel
- 7B0S 3.6 Å, TRPC4 in complex with inhibitor GFB-8438
- 7B05 3.8 Å, TRPC4 in complex with inhibitor GFB-8749
Browse structure collections
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