Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and mant-ADP. Determined by X-ray diffraction at 2.69 Å resolution. Released 9 Jun 2021.
Explore 7BDL in 3D Show helices and sheets RCSB PDB PDBe
7BDL contains 106 α-helices and 82 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 411-414 | 4 | |
| α-helix | 419-421 | 3 | |
| α-helix | 429-431 | 3 | |
| β-strand | 435-438 | 4 | 1 |
| β-strand | 442-447 | 6 | 1 |
| α-helix | 448-454 | 7 | |
| β-strand | 463 | 1 | 2 |
| α-helix | 464-466 | 3 | |
| α-helix | 469-475 | 7 | |
| β-strand | 480 | 1 | 2 |
| α-helix | 483-486 | 4 | |
| α-helix | 489-493 | 5 | |
| β-strand | 499-502 | 4 | 3 |
| α-helix | 509-521 | 13 | |
| α-helix | 522-524 | 3 | |
| β-strand | 538-541 | 4 | 3 |
| α-helix | 545-558 | 14 | |
| α-helix | 560-562 | 3 | |
| β-strand | 566-568 | 3 | 3 |
| α-helix | 578-582 | 5 | |
| β-strand | 585-588 | 4 | 3 |
| α-helix | 590-598 | 9 | |
| α-helix | 606-608 | 3 | |
| β-strand | 611-614 | 4 | 3 |
| α-helix | 617-621 | 5 | |
| α-helix | 625-640 | 16 | |
| β-strand | 647-652 | 6 | 3 |
| α-helix | 658-664 | 7 | |
| β-strand | 673-675 | 3 | 3 |
| α-helix | 678-680 | 3 | |
| β-strand | 685-692 | 8 | 1 |
| α-helix | 697-712 | 16 | |
| β-strand | 721-724 | 4 | 1 |
| α-helix | 730-745 | 16 | |
| α-helix | 759-767 | 9 | |
| α-helix | 772-775 | 4 | |
| α-helix | 778-780 | 3 | |
| β-strand | 782-785 | 4 | 1 |
| α-helix | 792-802 | 11 | |
| β-strand | 808-811 | 4 | 1 |
| α-helix | 813-818 | 6 | |
| β-strand | 823 | 1 | 4 |
| β-strand | 825-829 | 5 | 1 |
| β-strand | 832-835 | 4 | 5 |
| β-strand | 840-843 | 4 | 5 |
| α-helix | 844-845 | 2 | |
| α-helix | 846-854 | 9 | |
| β-strand | 856 | 1 | 4 |
| β-strand | 865-872 | 8 | 1 |
| α-helix | 873-875 | 3 | |
| α-helix | 876-882 | 7 | |
| α-helix | 897-907 | 11 | |
| β-strand | 912 | 1 | 6 |
| α-helix | 913-920 | 8 | |
| α-helix | 924-931 | 8 | |
| α-helix | 933-936 | 4 | |
| α-helix | 940-943 | 4 | |
| α-helix | 950-966 | 17 | |
| β-strand | 970-972 | 3 | 7 |
| β-strand | 978 | 1 | 6 |
| β-strand | 979-981 | 3 | 7 |
| α-helix | 983-990 | 8 | |
| α-helix | 995-1004 | 10 | |
| α-helix | 1011-1019 | 9 | |
| α-helix | 1022-1024 | 3 | |
| α-helix | 1034-1041 | 8 | |
| α-helix | 1055-1067 | 13 | |
| α-helix | 1075-1086 | 12 | |
| α-helix | 1088-1100 | 13 | |
| β-strand | 1104 | 1 | 8 |
| α-helix | 1105-1120 | 16 | |
| α-helix | 1128-1131 | 4 | |
| α-helix | 1137-1144 | 8 | |
| α-helix | 1150-1152 | 3 | |
| α-helix | 1158-1164 | 7 | |
| α-helix | 1171-1180 | 10 | |
| β-strand | 1184-1192 | 9 | 9 |
| β-strand | 1197-1206 | 10 | 9 |
| β-strand | 1218-1226 | 9 | 10 |
| β-strand | 1232 | 1 | 8 |
| β-strand | 1233-1242 | 10 | 10 |
| α-helix | 1243-1245 | 3 | |
| β-strand | 1250-1257 | 8 | 9 |
| β-strand | 1265-1272 | 8 | 10 |
| β-strand | 1279-1285 | 7 | 10 |
| α-helix | 1291-1300 | 10 | |
| α-helix | 1304-1307 | 4 | |
| β-strand | 1308 | 1 | 11 |
| α-helix | 1309-1312 | 4 | |
| α-helix | 1315-1318 | 4 | |
| β-strand | 1327 | 1 | 11 |
| α-helix | 1330-1340 | 11 | |
| β-strand | 1346-1349 | 4 | 12 |
| α-helix | 1356-1370 | 15 | |
| β-strand | 1376-1379 | 4 | 12 |
| α-helix | 1383-1393 | 11 | |
| α-helix | 1394-1400 | 7 | |
| β-strand | 1405-1407 | 3 | 12 |
| α-helix | 1412-1421 | 10 | |
| β-strand | 1424-1427 | 4 | 12 |
| α-helix | 1429-1437 | 9 | |
| α-helix | 1443-1446 | 4 | |
| β-strand | 1450-1453 | 4 | 12 |
| α-helix | 1456-1460 | 5 | |
| α-helix | 1464-1480 | 17 | |
| β-strand | 1486-1491 | 6 | 12 |
| β-strand | 1494 | 1 | 13 |
| α-helix | 1497-1504 | 8 | |
| β-strand | 1511-1513 | 3 | 12 |
| α-helix | 1516-1518 | 3 | |
| β-strand | 1523-1530 | 8 | 14 |
| α-helix | 1535-1553 | 19 | |
| β-strand | 1559-1563 | 5 | 14 |
| α-helix | 1566-1582 | 17 | |
| β-strand | 1590 | 1 | 15 |
| α-helix | 1594-1596 | 3 | |
| α-helix | 1598-1601 | 4 | |
| α-helix | 1608-1614 | 7 | |
| β-strand | 1617-1620 | 4 | 14 |
| α-helix | 1626-1637 | 12 | |
| β-strand | 1641 | 1 | 15 |
| β-strand | 1643-1647 | 5 | 14 |
| α-helix | 1648-1650 | 3 | |
| β-strand | 1658 | 1 | 16 |
| β-strand | 1660-1664 | 5 | 14 |
| β-strand | 1667-1670 | 4 | 17 |
| β-strand | 1675-1678 | 4 | 17 |
| α-helix | 1679-1680 | 2 | |
| α-helix | 1681-1688 | 8 | |
| β-strand | 1691 | 1 | 16 |
| β-strand | 1700-1707 | 8 | 14 |
| α-helix | 1708-1710 | 3 | |
| α-helix | 1711-1716 | 6 | |
| α-helix | 1721 | 1 | |
| β-strand | 1722 | 1 | 13 |
| α-helix | 1723 | 1 | |
| α-helix | 1728-1730 | 3 | |
| α-helix | 1732-1741 | 10 | |
| β-strand | 1747 | 1 | 18 |
| α-helix | 1748-1755 | 8 | |
| α-helix | 1759-1766 | 8 | |
| α-helix | 1768-1771 | 4 | |
| α-helix | 1778-1798 | 21 | |
| β-strand | 1802-1805 | 4 | 18 |
| β-strand | 1809-1812 | 4 | 18 |
| α-helix | 1814-1821 | 8 | |
| α-helix | 1826-1834 | 9 | |
| α-helix | 1842-1850 | 9 | |
| α-helix | 1853-1855 | 3 | |
| α-helix | 1865-1874 | 10 | |
| α-helix | 1889-1899 | 11 | |
| α-helix | 1909-1932 | 24 | |
| α-helix | 1937-1951 | 15 | |
| α-helix | 1982-1986 | 5 | |
| α-helix | 1990-1997 | 8 | |
| α-helix | 2004-2011 | 8 | |
| β-strand | 2017-2023 | 7 | 19 |
| β-strand | 2035-2043 | 9 | 19 |
| β-strand | 2064-2069 | 6 | 20 |
| β-strand | 2078-2082 | 5 | 20 |
| β-strand | 2087-2094 | 8 | 19 |
| β-strand | 2100-2110 | 11 | 20 |
| β-strand | 2118-2126 | 9 | 20 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 2073-2080 | 8 | |
| α-helix | 2083-2086 | 4 | |
| β-strand | 2090-2092 | 3 | 21 |
| β-strand | 2103-2107 | 5 | 21 |
| α-helix | 2108-2116 | 9 | |
| β-strand | 2125-2131 | 7 | 21 |
| β-strand | 2139-2146 | 8 | 21 |
| β-strand | 2150 | 1 | 22 |
| β-strand | 2151-2152 | 2 | 23 |
| β-strand | 2157-2158 | 2 | 23 |
| α-helix | 2163-2164 | 2 | |
| β-strand | 2173-2181 | 9 | 21 |
| α-helix | 2190-2202 | 13 | |
| β-strand | 2212-2217 | 6 | 21 |
| β-strand | 2223-2230 | 8 | 21 |
| α-helix | 2232-2238 | 7 | |
| α-helix | 2253-2255 | 3 | |
| β-strand | 2256-2264 | 9 | 21 |
| β-strand | 2270-2272 | 3 | 21 |
| β-strand | 2273-2274 | 2 | 24 |
| β-strand | 2280 | 1 | 22 |
| β-strand | 2296-2297 | 2 | 24 |
| α-helix | 2300-2303 | 4 | |
| α-helix | 2307-2309 | 3 | |
| α-helix | 2311-2318 | 8 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| U5 small nuclear ribonucleoprotein 200 kDa helicase | B | protein | 1747 | Homo sapiens | O75643 (AlphaFold model) |
| Pre-mRNA-processing-splicing factor 8 | J | protein | 263 | Homo sapiens | Q6P2Q9 (AlphaFold model) |
>7BDL_1 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains B) GAEFMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPA LKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGK TNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAEL TGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLE ALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTY VGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLG LFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVL VSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGIL ITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRM LRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIAS HYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVK ESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTD KTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPK MGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVD SEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHL ILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVG APTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLT GETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEV ICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQG FNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRF LHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRS LCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGS KKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPN YYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTI ELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHV KTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMV TQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVAR FCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIG DAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEA ETDSDSD
>7BDL_2 Pre-mRNA-processing-splicing factor 8 (chains J) GPLGSMTQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICI SDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGW IHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEW GRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQ LANPKEFYHEVHRPSHFLNFALL
| ID | Name | Formula | Copies |
|---|---|---|---|
| TG8 | [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-[[oxidanyl(phosph… | C18 H22 N6 O11 P2 | 2 |
| MG | Magnesium ion | Mg | 2 |
Long-range allostery mediates cooperative adenine nucleotide binding by the Ski2-like RNA helicase Brr2. Absmeier, E., Vester, K., Ghane, T. et al. J Biol Chem (2021) 297:100829-100829. DOI 10.1016/j.jbc.2021.100829 · PubMed
Other PDB entries of the same protein (UniProt O75643 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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