7C8D: Cat ACE2 and SARS-CoV-2 RBD

Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD. Determined by electron microscopy at 3.0 Å resolution. Released 2 Sept 2020.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Felis catus, Severe acute respiratory syndrome coronavirus 2
Chains: 2
Atoms: 6,359
Mol. weight: 107.07 kDa
Ligands: ZN
Released: 2 Sept 2020

Explore 7C8D in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7C8D contains 42 α-helices and 23 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 36 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	21-52	32
α-helix	57-80	24
α-helix	85-87	3
α-helix	91-101	11
α-helix	110-127	18
β-strand	131-133	3	1
β-strand	141-143	3	1
α-helix	144-148	5
α-helix	149-154	6
α-helix	158-170	13
α-helix	177-193	17
α-helix	199-204	6
β-strand	209	1	2
β-strand	217	1	2
α-helix	221-251	31
β-strand	262-263	2	3
α-helix	276-278	3
α-helix	279-282	4
α-helix	289-290	2
α-helix	294-299	6
α-helix	304-317	14
α-helix	320-324	5
α-helix	327-330	4
β-strand	348-350	3	4
β-strand	356-358	3	4
α-helix	367-384	18
α-helix	385-387	3
α-helix	390-392	3
α-helix	401-412	12
α-helix	415-418	4
α-helix	434-446	13
α-helix	447-449	3
α-helix	450-465	16
α-helix	473-480	8
α-helix	481-485	5
β-strand	487-488	2	3
α-helix	499-502	4
α-helix	504-507	4
α-helix	514-532	19
α-helix	548-558	11
α-helix	561-563	3
α-helix	566-574	9
α-helix	582-587	6
α-helix	589-598	10

Chain B: 6 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	339-342	4
β-strand	349	1	5
α-helix	353	1
β-strand	354-358	5	5
α-helix	366-369	4
β-strand	376-380	5	5
β-strand	391-392	2	6
β-strand	394-403	10	5
α-helix	404-409	6
α-helix	417	1
α-helix	418-422	5
β-strand	431-437	7	5
β-strand	448	1	7
β-strand	452-454	3	8
β-strand	473-474	2	9
β-strand	485	1	9
β-strand	488-489	2	9
β-strand	492-494	3	8
β-strand	497	1	7
β-strand	507-516	10	5
β-strand	524-525	2	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Angiotensin-converting enzyme 2	A	protein	732	Felis catus	Q56H28 (AlphaFold model)
Spike protein S1	B	protein	195	Severe acute respiratory syndrome coronavirus 2	P0DTC2 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7C8D_1 Angiotensin-converting enzyme 2 (chains A)
MQSTTEELAKTFLEKFNHEAEELSYQSSLASWNYNTNITDENVQKMNEAGAKWSAFYEEQ
SKLAKTYPLAEIHNTTVKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPN
NPQECLLLEPGLDDIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNY
EDYGDYWRGDYEEEWTDGYNYSRSQLIKDVEHTFTQIKPLYQHLHAYVRAKLMDTYPSRI
SPTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQSWDARRIFKEAEKFFV
SVGLPNMTQGFWENSMLTEPGDSRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEM
GHIQYDMAYAVQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPGFSEDSETEI
NFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMQKWWEMKREIVGVVEPVPHDET
YCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCRIAKHEGPLHKCDISNSSEAGKKLLQ
MLTLGKSKPWTLALEHVVGEKKMNVTPLLKYFEPLFTWLKEQNRNSFVGWNTDWRPYADQ
SIKVRISLKSALGDEAYEWNDNEMYLFRSSVAYAMREYFSKVKNQTIPFVEDNVWVSNLK
PRISFNFFVTASKNVSDVIPRSEVEEAIRMSRSRINDAFRLDDNSLEFLGIQPTLSPPYQ
PPVTHHHHHHHH

Sequence of entity 2 (B), FASTA

>7C8D_2 Spike protein S1 (chains B)
TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCF
TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYL
YRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVV
LSFELLHAPATVCGP

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1

Primary citation

Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2. Wu, L., Chen, Q., Liu, K. et al. Cell Discov (2020) 6:68-68. DOI 10.1038/s41421-020-00210-9 · PubMed

Other PDB entries of the same protein (UniProt Q56H28 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9DAK 2.4 Å, Merbecovirus PnNL2018B Spike glycoprotein RBD bound to the P. Nathusii ACE2
9N7E 2.5 Å, Eptesicus fuscus ACE2 peptidase domain bound to VsCoV-a7 RBD complex
9C6O 2.77 Å, Merbecovirus MOW15-22 Spike glycoprotein RBD bound to the P. davyi ACE2
9KWY 2.82 Å, Cryo-EM structure of SARS-CoV-2 RBD in complex with ACE2 and mAb 1C4
8ZUF 3.31 Å, Cryo-EM structure of P.nat ACE2 mutant in complex with MOW15-22 RBD

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