8ZUF: P.nat ACE2 mutant

Cryo-EM structure of P.nat ACE2 mutant in complex with MOW15-22 RBD. Determined by electron microscopy at 3.31 Å resolution. Released 12 Feb 2025.

Method: Electron microscopy
Resolution: 3.31 Å
Organisms: Pipistrellus nathusii, Middle East respiratory syndrome-related coronavirus
Chains: 2
Atoms: 5,745
Mol. weight: 118.24 kDa
Ligands: ZN, NAG
Released: 12 Feb 2025

Explore 8ZUF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZUF contains 46 α-helices and 20 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	24-52	29
α-helix	57-80	24
α-helix	81-83	3
α-helix	86-88	3
α-helix	92-102	11
α-helix	105-108	4
α-helix	111-129	19
β-strand	132-135	4	1
β-strand	138-144	7	1
α-helix	145-152	8
α-helix	158-168	11
α-helix	169-173	5
α-helix	174-192	19
α-helix	199-203	5
β-strand	209	1	2
β-strand	217	1	2
α-helix	219-251	33
α-helix	261	1
β-strand	262-263	2	3
α-helix	264-266	3
α-helix	276-278	3
α-helix	279-282	4
α-helix	294-299	6
α-helix	304-316	13
α-helix	321-324	4
α-helix	325-330	6
β-strand	332	1	4
β-strand	347-350	4	4
β-strand	356-359	4	4
α-helix	366-384	19
α-helix	390-392	3
α-helix	400-412	13
α-helix	415-420	6
α-helix	432-446	15
α-helix	449-465	17
α-helix	470-472	3
α-helix	473-480	8
α-helix	481-485	5
β-strand	487-488	2	3
α-helix	499-502	4
α-helix	504-507	4
α-helix	513-532	20
α-helix	539-541	3
α-helix	548-558	11
α-helix	566-574	9
α-helix	582-587	6
α-helix	589-599	11

Chain B: 8 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	396-398	3
β-strand	400-402	3	5
α-helix	411-414	4
β-strand	419-424	6	5
β-strand	442-447	6	5
α-helix	453-456	4
α-helix	464-468	5
β-strand	479-484	6	5
β-strand	497-508	12	6
β-strand	518-525	8	6
α-helix	533-535	3
α-helix	536-548	13
β-strand	552-557	6	6
β-strand	562	1	7
α-helix	567	1
β-strand	568	1	7
α-helix	569	1
β-strand	570-578	9	6
β-strand	586-593	8	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Angiotensin-converting enzyme	A	protein	782	Pipistrellus nathusii	Q56H28 (AlphaFold model)
MOW15-22 rbd	B	protein	213	Middle East respiratory syndrome-related coronavirus	A3EXD0 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8ZUF_1 Angiotensin-converting enzyme (chains A)
EEKAREFLDKFNSEAENWSHESALASWDYNTNINDKNAQKMNEADSKWSAFYKEHSKLAQ
GFPLQEIQNSTIKLQLQILQQNGSSVLTAEKSKRLSTILTTMSTIYSTGKVCNPNNPQQC
FTLSGLEDIMEKSKDYHQRLWIWEGWRSEVGKQLRPLYEEYVALKNEMARGNNYKDYGDY
WRGDYETEGGDGYNYSRNHLIEDVDRIFLEIKPLYEQLHAYVRAKLMNAYPSRISPTGCL
PAHLLGDMWGRFWTNLYNLTVPFEKKQNIDVTDTMKKQSWDAEKIFKEAEKFYLSVGLHN
MTPEFWNNSMLTEPSDGRQVVCHPTAWDLGKNDFRIKMCTKVTMDDFLTAHHEMGHIQYD
MAYAKQPYLLRNGANEGFHEAVGEIMSLSAATPKHLKDLGLLAQNYPEDYETEINFLLKQ
ALNIVGTLPFTYMLEKWRWMVFEGKIPKEQWMEKWWEMKREIVGVVEPLPHDETYCDPAS
LFHVANDYSFIRYFTRTILEFQFQEALCQIANHTGPLHKCDISNSTEAGKQLKNMLELGK
SKPWTFALEQIARTKEMDAKPLLNYFKPLFSWLKELNGNSVGWSADWSPYSEQSIKVRIS
LKSALGEKAYEWNDNEMYLFRSSVAYAMRVYFLKVKNETIPFRAEDVWVSDEKIRVSFKF
FVTSPTNVSDIIPRSEVEDAIRMSRGRINDAFRLDDKTLEFLGIQPTLGPPYQPPVTIWL
IVFGVVMGMVVIGIGVLIFTGIRDRKKKNQAENEENPYSSVNLSKGENNPGFQSGDDVQT
SF

Sequence of entity 2 (B), FASTA

>8ZUF_2 MOW15-22 RBD (chains B)
ECDFTKLFIGQVPQPYEFGRLVFTNCNYNFTKLLSYFQVNTFQCQKVTPESIATGCYSSL
TVDWFAYRVEDKSDLLPGSSSDLQRFNYKPTYSNPTCLISAYTNLVPLGGVNPTNYTTLT
NCYGCVDKDPANPWGDQICIPEFVTEVEPGFRPKPSCARVGLEGHISGNDTYSAIVTNGE
LDSTGDPIWRKGVALTKQPIDSSRADLAFFVSV

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	4

Primary citation

Multiple independent acquisitions of ACE2 usage in MERS-related coronaviruses. Ma, C.B., Liu, C., Park, Y.J. et al. Cell (2025) 188:1693. DOI 10.1016/j.cell.2024.12.031 · PubMed

Other PDB entries of the same protein (UniProt Q56H28 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9DAK 2.4 Å, Merbecovirus PnNL2018B Spike glycoprotein RBD bound to the P. Nathusii ACE2
9N7E 2.5 Å, Eptesicus fuscus ACE2 peptidase domain bound to VsCoV-a7 RBD complex
9C6O 2.77 Å, Merbecovirus MOW15-22 Spike glycoprotein RBD bound to the P. davyi ACE2
9KWY 2.82 Å, Cryo-EM structure of SARS-CoV-2 RBD in complex with ACE2 and mAb 1C4
7C8D 3.0 Å, Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD

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