7CEI: The endonuclease domain of colicin E7

The endonuclease domain of colicin E7 in complex with its inhibitor IM7 protein. Determined by X-ray diffraction at 2.3 Å resolution. Released 17 Sept 1999.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Escherichia coli str. K12 substr.
Chains: 2
Atoms: 1,887
Mol. weight: 33.44 kDa
Ligands: ZN
Released: 17 Sept 1999

Explore 7CEI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7CEI contains 11 α-helices and 13 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	12-26	15
α-helix	34-45	12
α-helix	52-55	4
α-helix	65-77	13

Chain B: 7 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	452	1	1
β-strand	454	1	2
β-strand	458	1	3
β-strand	474	1	4
β-strand	477	1	3
α-helix	478-484	7
β-strand	488	1	1
α-helix	492-503	12
α-helix	510-512	3
α-helix	515-522	8
α-helix	525-527	3
β-strand	528	1	5
α-helix	531-533	3
β-strand	535	1	6
β-strand	538	1	6
β-strand	540	1	5
β-strand	542-545	4	4
β-strand	557	1	2
β-strand	561-564	4	4
α-helix	566-571	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein (colicin E7 immunity protein)	A	protein	87	Escherichia coli str. K12 substr.	Q03708 (AlphaFold model)
Protein (colicin E7 immunity protein)	B	protein	206	Escherichia coli str. K12 substr.	Q47112 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7CEI_1 PROTEIN (COLICIN E7 IMMUNITY PROTEIN) (chains A)
MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKITEHPDGTDLIYYPSDN
RDDSPEGIVKEIKEWRAANGKPGFKQG

Sequence of entity 2 (B), FASTA

>7CEI_2 PROTEIN (COLICIN E7 IMMUNITY PROTEIN) (chains B)
ERFAREPMAAGHRMWQMAGLKAQRAQTDVNNKKAAFDAAAKEKSDADVALSSALERRKQK
ENKEKDAKAKLDKESKRNKPGKATGKGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEFK
SFDDFRKKFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELHHEKPIS
QNGGVYDMDNISVVTPKRHIDIHRGK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1

Primary citation

The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein. Ko, T.P., Liao, C.C., Ku, W.Y. et al. Structure (1999) 7:91-102. DOI 10.1016/S0969-2126(99)80012-4 · PubMed

Other PDB entries of the same protein (UniProt Q03708 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5WNW 1.79 Å, Chaperone Spy bound to Im7 6-45 ensemble
1CEI 1.8 Å, Structure determination of the colicin E7 immunity protein (IMME7) that binds…
1UNK 1.8 Å, Structure of colicin E7 immunity protein
2JB0 1.91 Å, Crystal structure of the mutant H573A of the nuclease domain of COLE7 in complex with IM7
1AYI 2.0 Å, Colicin E7 immunity protein IM7
1MZ8 2.0 Å, Crystal structures of the nuclease domain of COLE7/IM7 in complex with a phosphate ion…
1ZNV 2.0 Å, How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal…
2ERH 2.0 Å, Crystal Structure of the E7_G/Im7_G complex; a designed interface between the colicin E7…
2JAZ 2.03 Å, Crystal structure of the mutant N560D of the nuclease domain of COLE7 in complex with IM7
1UJZ 2.1 Å, Crystal structure of the E7_C/Im7_C complex; a computationally designed interface…
2JBG 2.2 Å, crystal structure of the mutant N560A of the nuclease domain of ColE7 in complex with Im7
4F37 2.57 Å, Structure of the tethered N-terminus of Alzheimer's disease A peptide

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