7DL2: Human TSC complex

Cryo-EM structure of human TSC complex. Determined by electron microscopy at 4.4 Å resolution. Released 16 Dec 2020.

Method
Electron microscopy
Resolution
4.4 Å
Organism
Homo sapiens
Chains
6
Atoms
23,285
Mol. weight
689.4 kDa
Released
16 Dec 2020

Explore 7DL2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7DL2 contains 150 α-helices and 38 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 58 helices, 17 β-strands

ElementResiduesLengthSheet
α-helix143-1464
α-helix154-1629
α-helix178-1825
α-helix185-1917
α-helix203-21412
α-helix222-2309
α-helix231-2333
α-helix242-2476
α-helix257-26711
α-helix274-2807
α-helix289-29911
α-helix300-3023
α-helix316-32611
α-helix332-34514
α-helix360-37617
α-helix385-39814
α-helix399-4024
α-helix408-4158
α-helix423-43412
α-helix442-4498
α-helix450-4545
α-helix459-47517
α-helix482-4898
α-helix501-51717
α-helix523-53513
α-helix543-5453
α-helix550-57425
α-helix581-60020
α-helix605-61915
β-strand622-62321
β-strand630-63121
α-helix687-69913
α-helix704-71815
α-helix720-7256
α-helix731-74111
α-helix759-77315
α-helix777-7793
α-helix782-79413
α-helix802-81413
α-helix816-82914
α-helix840-85112
α-helix862-87211
α-helix873-8764
α-helix883-89917
α-helix908-91912
α-helix927-9282
α-helix1016-103318
β-strand103812
α-helix1046-10516
β-strand1056-106163
β-strand1067-107153
α-helix1077-10815
α-helix1183-11853
α-helix1187-11926
β-strand1195-120173
β-strand1204-121073
β-strand1211-121224
α-helix1234-12385
α-helix1498-15047
β-strand1518-151923
α-helix1520-15212
α-helix1525-153612
β-strand1541-154995
α-helix1569-15768
β-strand158015
β-strand1609-161135
β-strand1615-162175
α-helix1637-16426
β-strand1646-165055
α-helix1659-16613
β-strand1669-167575
β-strand1681-168665
β-strand1700-170345
α-helix1708-172720
α-helix1739-175416
Chain B: 53 helices, 19 β-strands
ElementResiduesLengthSheet
α-helix125-13915
α-helix161-17010
α-helix187-19812
α-helix212-22110
α-helix235-24713
α-helix266-2727
α-helix282-2887
α-helix294-30411
α-helix319-3279
α-helix332-34514
α-helix360-37617
α-helix383-39816
α-helix410-4178
α-helix426-4338
α-helix444-4507
α-helix459-46810
α-helix477-4815
α-helix482-49211
α-helix501-51616
α-helix523-53412
α-helix549-57325
α-helix580-59920
α-helix605-61814
α-helix688-69811
α-helix705-71814
α-helix722-7243
α-helix730-73910
α-helix759-77315
α-helix782-79615
α-helix802-81413
α-helix816-83015
α-helix840-85112
α-helix862-87211
α-helix873-8764
α-helix883-89917
α-helix908-91710
α-helix1016-103318
α-helix1046-10527
β-strand1056-106054
β-strand1066-107164
α-helix1075-10817
α-helix1181-11844
α-helix1187-11915
β-strand1194-120184
β-strand1204-121184
β-strand121213
α-helix12151
β-strand121612
α-helix12171
α-helix1229-124113
α-helix1498-15047
α-helix1513-15153
β-strand151914
α-helix1525-153612
β-strand154116
β-strand154417
β-strand1547-154938
α-helix1569-157810
β-strand158017
β-strand1609-161027
β-strand161516
β-strand1617-161827
β-strand162118
α-helix1637-16437
β-strand1647-165048
α-helix1659-16613
β-strand1670-167458
β-strand1681-168668
β-strand1701-170338
α-helix1705-17073
α-helix1708-172922
α-helix1739-175416
Chain C: 3 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix747-84094
α-helix842-87029
α-helix876-96691
Chain D: 2 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix747-869123
α-helix875-96995
Chain E: 21 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix22-3110
α-helix40-489
α-helix50-523
α-helix53-6311
α-helix71-733
α-helix74-9522
α-helix104-11512
α-helix129-14416
α-helix148-16013
α-helix161-1655
α-helix166-1683
α-helix173-18210
α-helix187-1959
α-helix198-2003
α-helix204-2074
β-strand21219
β-strand21519
α-helix217-2193
α-helix221-2288
α-helix234-24512
α-helix247-2515
α-helix256-2649
α-helix271-28515
Chain F: 13 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix19-3315
α-helix41-5010
α-helix57-648
α-helix70-7910
α-helix84-9512
α-helix124-1329
α-helix144-15512
α-helix164-1696
α-helix186-1905
α-helix197-20812
α-helix217-2259
α-helix231-2366
α-helix246-25914

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
HamartinC, Dprotein1164Homo sapiensQ92574 (AlphaFold model)
Isoform 7 of TuberinA, Bprotein1692Homo sapiensP49815 (AlphaFold model)
TBC1 domain family member 7Eprotein267Homo sapiensQ9P0N9 (AlphaFold model)
unknown proteinFprotein261Homo sapiens
Sequence of entity 1 (C, D), FASTA
>7DL2_1 Hamartin (chains C, D)
MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNTLVDYYLETSSQPALHI
LTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPSWKHKLSQAPLLPSLLKCL
KMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVAEVYLVHL
HASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDH
ELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTTSPYADT
QNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPT
SPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLPQATVTPPR
KEERMDSARPCLHRQHHLLNDRGSEEPPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEA
AISRELSEITTAEAEPVVPRGGFDSPFYRDSLPGSQRKTHSAASSSQGASVNPEPLHSSL
DKLGPDTPKQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQ
PPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAH
SKELNKLPLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLL
RKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIR
QLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSES
VQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQ
RLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFEL
EILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKT
PRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGS
LPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHP
SPPTPDSVGQLHIMDYNETHHEHS
Sequence of entity 2 (A, B), FASTA
>7DL2_2 Isoform 7 of Tuberin (chains A, B)
MECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIV
QGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMD
VGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYN
CLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDA
PLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKY
RKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFE
LVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVL
SFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD
IIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEM
LVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYM
EPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLV
LGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPV
LTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVK
LTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLA
HHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQ
TSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLL
AGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKL
ESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAG
TRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELS
NALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPP
GLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLS
FQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGE
DSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGI
NPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQS
NSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIM
QAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTP
LDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDI
YPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKR
LISSVEDFTEFV
Sequence of entity 3 (E), FASTA
>7DL2_3 TBC1 domain family member 7 (chains E)
GVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVWKVLLGILPPHHESHAKVMMY
RKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLPRSPSFPLEPDDEVFLAIAKA
MEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLPKAFEQYLNLEDGRLLTHLRMCSAAPK
LPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSAEKIT
KFLENIPQDSSDAIVSKAIDLWHKHCG
Sequence of entity 4 (F), FASTA
>7DL2_4 unknown protein (chains F)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXX

Primary citation

Structural insights into TSC complex assembly and GAP activity on Rheb. Yang, H., Yu, Z., Chen, X. et al. Nat Commun (2021) 12:339-339. DOI 10.1038/s41467-020-20522-4 · PubMed

Other PDB entries of the same protein (UniProt Q92574 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7DL2 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.