7EJA: PDB entry 7EJA

Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to dexmedetomidine. Determined by electron microscopy at 3.6 Å resolution. Released 13 Apr 2022.

Method: Electron microscopy
Resolution: 3.6 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,584
Mol. weight: 170.17 kDa
Ligands: CZX
Released: 13 Apr 2022

Explore 7EJA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7EJA contains 29 α-helices and 62 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-38	5	1
α-helix	46-51	6
β-strand	186-189	4	1
β-strand	192	1	2
β-strand	195	1	2
β-strand	197-201	5	1
α-helix	209-213	5
α-helix	214-217	4
β-strand	221-226	6	1
α-helix	246-255	10
β-strand	264-269	6	1
α-helix	273-280	8
α-helix	298-306	9
β-strand	320	1	1
β-strand	323	1	1
α-helix	330-349	20

Chain B: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	8-24	17
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	70-74	5	4
β-strand	78-83	6	4
β-strand	88-94	7	4
α-helix	95	1
β-strand	102-105	4	5
β-strand	111-115	5	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	157-161	5	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	191-192	2	7
β-strand	198-201	4	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	241-245	5	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	275-277	3	9
β-strand	284-288	5	9
β-strand	293	1	10
β-strand	296-298	3	9
β-strand	306	1	9
β-strand	309	1	10
β-strand	317-320	4	3
β-strand	327-330	4	3
β-strand	336-339	4	3

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	10-23	14
α-helix	27-29	3
α-helix	30-44	15
α-helix	53-55	3

Chain H: 2 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	11
β-strand	12	1	12
β-strand	17-25	9	11
α-helix	29-31	3
β-strand	33-39	7	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-84	7	11
α-helix	88-90	3
β-strand	93-99	7	13
β-strand	111	1	13
β-strand	119	1	12
β-strand	129	1	14
β-strand	134-136	3	15
β-strand	143-148	6	14
β-strand	154	1	16
β-strand	160	1	16
β-strand	162-167	6	15
β-strand	174-178	5	15
β-strand	191-195	5	14
β-strand	199-204	6	14
β-strand	214-219	6	15
β-strand	227	1	15
β-strand	231-234	4	15

Chain R: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	48-73	26
α-helix	84-107	24
α-helix	121-150	30
α-helix	153-156	4
α-helix	157-159	3
α-helix	162-180	19
α-helix	209-216	8
α-helix	217-221	5
α-helix	222-241	20
α-helix	377-410	34
α-helix	423-442	20
α-helix	446-456	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(o) subunit alpha	A	protein	354	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	349	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Alpha-2A adrenergic receptor	R	protein	465	Homo sapiens	P08913 (AlphaFold model)
scFv16	H	protein	307	Mus musculus

Sequence of entity 1 (A), FASTA

>7EJA_1 Guanine nucleotide-binding protein G(o) subunit alpha (chains A)
MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDG
FSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPF
SAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRV
KTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDET
TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDA
AAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY

Sequence of entity 2 (B), FASTA

>7EJA_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTL
RGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNY
VACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDI
ETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINA
ICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDD
FNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7EJA_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (R), FASTA

>7EJA_4 Alpha-2A adrenergic receptor (chains R)
MFRQEQPLAEGSFAPMGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLLML
LTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFGKAW
CEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVISAVIS
FPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKR
RTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGP
RDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTP
AAGPGEERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPR
TLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV

Sequence of entity 5 (H), FASTA

>7EJA_5 scFv16 (chains H)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFADVQLVESGGGLVQPGGSRKLSC
SASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQ
MTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELKGSLEVLFQGPAAAH
HHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
CZX	4-[(1~{S})-1-(2,3-dimethylphenyl)ethyl]-1~{H}-imidazole	C13 H16 N2	1

Primary citation

Structural insights into ligand recognition, activation, and signaling of the alpha 2A adrenergic receptor. Xu, J., Cao, S., Hubner, H. et al. Sci Adv (2022) 8:eabj5347-eabj5347. DOI 10.1126/sciadv.abj5347 · PubMed

Other PDB entries of the same protein (UniProt P09471 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BSB 2.32 Å, Global reconstruction of DRD2 bound to LSD in complex with a mini-GoA and scFv16…
9VJE 2.47 Å, Cryo-EM structure of 5-HT1AR-GoA in complex with buspirone
9K20 2.65 Å, Cryo-EM structure of ATP-bound P2Y purinoceptor 2-miniGo-scFv16 complex
9VJ5 2.69 Å, Cryo-EM structure of 5-HT1AR-GoA in complex with gepirone
8E9X 2.7 Å, CryoEM structure of miniGo-coupled hM4Di in complex with DCZ
9T88 2.74 Å, Cryo-EM structure of ESG-2-37-bound D3 dopamine receptor with mini-Go
9VNF 2.74 Å, Cryo-EM structure of 5-HT1AR-GoA in complex with (S)-pindolol
8YN7 2.77 Å, Cryo-EM structure of histamine H3 receptor in complex with immethridine and miniGo
8YN8 2.77 Å, Cryo-EM structure of histamine H3 receptor in complex with proxyfan and miniGo
9KVP 2.79 Å, Structure of SB290157 bound to human C3aR in complex with Go (Full map)
7W2Z 2.8 Å, Cryo-EM structure of the ghrelin-bound human ghrelin receptor-Go complex
8XXH 2.8 Å, Structure of CXCR2 bound to CXCL2 (CXCR2-CXCL2-Go Full map)

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