7F2O: Type 2 bradykinin receptor

Cryo-EM structure of the type 2 bradykinin receptor in complex with the bradykinin and an Gq protein. Determined by electron microscopy at 2.9 Å resolution. Released 13 Oct 2021.

Method: Electron microscopy
Resolution: 2.9 Å
Organisms: Homo sapiens, Rattus norvegicus, synthetic construct
Chains: 6
Atoms: 9,004
Mol. weight: 185.25 kDa
Released: 13 Oct 2021

Explore 7F2O in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F2O contains 36 α-helices and 61 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	1
α-helix	42-44	3
α-helix	46-53	8
β-strand	184-191	8	1
β-strand	194-201	8	1
α-helix	212-215	4
β-strand	220-226	7	1
α-helix	230-244	15
β-strand	253-259	7	1
α-helix	261-270	10
α-helix	275-278	4
α-helix	280-284	5
α-helix	299-317	19
β-strand	326	1	1
β-strand	329-331	3	1
α-helix	338-357	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	34-36	3
β-strand	46-51	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-340	5	2

Chain R: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	56-84	29
α-helix	88-90	3
α-helix	91-118	28
α-helix	126-159	34
α-helix	171-195	25
β-strand	196-201	6	9
β-strand	208-213	6	9
α-helix	218-227	10
α-helix	228-234	7
α-helix	235-256	22
α-helix	264-298	35
α-helix	304-321	18
α-helix	324-329	6
α-helix	330-334	5
α-helix	337-344	8

Chain S: 6 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	10-12	3	11
β-strand	19-25	7	10
α-helix	29-31	3
β-strand	33-39	7	11
β-strand	45-51	7	11
β-strand	59-60	2	11
β-strand	69-73	5	10
β-strand	78-82	5	10
α-helix	88-90	3
β-strand	92-99	8	11
β-strand	110-111	2	11
β-strand	115-119	5	11
β-strand	140-142	3	12
β-strand	146-148	3	13
β-strand	155-161	7	12
β-strand	166	1	14
β-strand	172	1	14
β-strand	174-179	6	13
α-helix	184-185	2
β-strand	186-191	6	13
β-strand	194-195	2	13
α-helix	196	1
β-strand	203-207	5	12
β-strand	211-216	6	12
α-helix	221-223	3
β-strand	226-231	6	13
α-helix	237	1
β-strand	238-239	2	13
β-strand	243-246	4	13

Chain Y: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	12-24	13
α-helix	30-43	14
α-helix	53-55	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
G subunit q (Gi1-Gq chimeric)	A	protein	361	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	377	Rattus norvegicus	P54311 (AlphaFold model)
Arg-pro-pro-gly-phe-ser-pro-phe-arg	D	protein	9	Homo sapiens	P01042 (AlphaFold model)
B2 bradykinin receptor	R	protein	595	Homo sapiens	P30411 (AlphaFold model)
single Fab chain (svFv16)	S	protein	248	synthetic construct
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	Y	protein	71	Bos taurus	P63212 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7F2O_1 G subunit q (Gi1-Gq chimeric) (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNL
V

Sequence of entity 2 (B), FASTA

>7F2O_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSGVSGWRLFKKIS

Sequence of entity 3 (D), FASTA

>7F2O_3 ARG-PRO-PRO-GLY-PHE-SER-PRO-PHE-ARG (chains D)
RPPGFSPFR

Sequence of entity 4 (R), FASTA

>7F2O_4 B2 bradykinin receptor (chains R)
ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKD
FRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLGTFAQSKCPQVEWL
GWLNTIQPPFLWVLFVLATLENIFVLSVFCLHKSSCTVAEIYLGNLAAADLILACGLPFW
AITISNNFDWLFGETLCRVVNAIISMNLYSSIWFLMLVSIDRYLALVKTMSMGRMRGVRW
AKLYSLVIWGCTLLLSSPMLVFRTMKEYSDEGHNVTACVISYPSLIWEVFTNMLLNVVGF
LLPLSVITFCTMQIMQVLRNNEMQKFKEIQTERRATVLVLVVLLLFIICWLPFQISTFLD
TLHRLGILSSCQDERIIDVITQIASFMAYSNSCLNPLVYVIVGKRFRKKSWEVYQGVCQK
GGCRSEPIQMENSMGTLVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQ
RIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGV
TPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINS

Sequence of entity 5 (S), FASTA

>7F2O_5 single Fab chain (svFv16) (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
AGGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQ
RPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTF
GAGTKLEL

Sequence of entity 6 (Y), FASTA

>7F2O_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains Y)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

Molecular basis for kinin selectivity and activation of the human bradykinin receptors. Yin, Y.L., Ye, C., Zhou, F. et al. Nat Struct Mol Biol (2021) 28:755-761. DOI 10.1038/s41594-021-00645-y · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF

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