7F8V: Cholecystokinin receptor CCKBR

Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gi. Determined by electron microscopy at 3.3 Å resolution. Released 13 Oct 2021.

Method: Electron microscopy
Resolution: 3.3 Å
Organism: Homo sapiens
Chains: 5
Atoms: 6,601
Mol. weight: 139.73 kDa
Released: 13 Oct 2021

Explore 7F8V in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F8V contains 29 α-helices and 45 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	11-28	18
β-strand	36	1	1
β-strand	37	1	2
β-strand	39	1	3
α-helix	46-51	6
β-strand	187-192	6	1
β-strand	195-200	6	1
β-strand	202	1	3
α-helix	209-211	3
α-helix	213-216	4
β-strand	221-227	7	2
α-helix	229-231	3
α-helix	233	1
β-strand	234	1	4
α-helix	235	1
β-strand	242	1	4
α-helix	243-255	13
α-helix	258-260	3
β-strand	264-270	7	2
α-helix	272-281	10
α-helix	297-309	13
β-strand	320-324	5	2
α-helix	332-352	21

Chain B: 0 helices, 32 β-strands

Element	Residues	Length	Sheet
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
β-strand	103-105	3	7
β-strand	111-114	4	7
β-strand	116	1	8
β-strand	121-125	5	7
β-strand	134-139	6	7
β-strand	146-151	6	8
β-strand	156-161	6	8
β-strand	165-170	6	8
β-strand	176-181	6	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	207-212	6	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	249	1	11
β-strand	250-254	5	10
β-strand	260-262	3	10
β-strand	265	1	11
β-strand	273-276	4	12
β-strand	287-289	3	12
β-strand	294	1	12
β-strand	295-296	2	13
β-strand	306-307	2	13
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	335-339	5	5

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	30-44	15
α-helix	53-55	3

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	57-80	24
α-helix	88-106	19
α-helix	108-116	9
α-helix	124-156	33
α-helix	159-165	7
α-helix	168-185	18
α-helix	188-191	4
β-strand	197-198	2	14
β-strand	202-203	2	14
α-helix	212-223	12
α-helix	224-228	5
α-helix	229-247	19
α-helix	328-359	32
α-helix	363-366	4
α-helix	370-386	17
α-helix	387-392	6
α-helix	395-404	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-2	A	protein	355	Homo sapiens	P04899 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
Gastrin-17	E	protein	17	Homo sapiens	P01350 (AlphaFold model)
Gastrin/cholecystokinin type B receptor	R	protein	465	Homo sapiens	P32239

Sequence of entity 1 (A), FASTA

>7F8V_1 Guanine nucleotide-binding protein G(i) subunit alpha-2 (chains A)
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVL
PDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRV
KTTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEE
MNRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEA
ASYIQSKFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 2 (B), FASTA

>7F8V_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>7F8V_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (E), FASTA

>7F8V_4 Gastrin-17 (chains E)
QGPWLEEEEEAYGWMDF

Sequence of entity 5 (R), FASTA

>7F8V_5 Gastrin/cholecystokinin type B receptor (chains R)
DYKDDDDGAPELLKLNRSVQGTGPGPGASLCRPGAPLLNSSSVGNLSCEPPRIRGAGTRE
LELAIRITLYAVIFLMSVGGNMLIIVVLGLSRRLRTVTNAFLLSLAVSDLLLAVACMPFT
LLPNLMGTFIFGTVICKAVSYLMGVSVSVSTLSLVAIALERYSAICRPLQARVWQTRSHA
ARVIVATWLLSGLLMVPYPVYTVVQPVGPRVLQCVHRWPSARVRQTWSVLLLLLLFFIPG
VVMAVAYGLISRELYLGLRFDGDSDSDSQSRVRNQGGLPGAVHQNGRCRPETGAVGEDSD
GCYVQLPRSRPALELTALTAPGPGSGSRPTQAKLLAKKRVVRMLLVIVVLFFLCWLPVYS
ANTWRAFDGPGAHRALSGAPISFIHLLSYASACVNPLVYCFMHRRFRQACLETCARCCPR
PPRARPREFLEVLFQGPWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Primary citation

Structures of the human cholecystokinin receptors bound to agonists and antagonists. Zhang, X., He, C., Wang, M. et al. Nat Chem Biol (2021) 17:1230-1237. DOI 10.1038/s41589-021-00866-8 · PubMed

Other PDB entries of the same protein (UniProt P04899 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8ZBW 2.58 Å, Cryo-EM structure of formyl peptide receptor 2/C1R receptor in complex with Gi
8THK 2.6 Å, Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with…
7YK7 2.75 Å, Cryo-EM structure of the DC591053-bound human relaxin family peptide receptor 4…
9K6L 2.77 Å, Cryo-EM structure of GPCR16-Gi2 complex
9XDQ 2.79 Å, Cryo-EM structure of BMS986187 bound DOR-Gi complex
7WVX 2.8 Å, Cryo-EM structure of the human formyl peptide receptor 2 in complex with fhumanin and Gi2
8W8A 2.8 Å, Cryo-EM structure of the RO5256390-TAAR1 complex
8ZSJ 2.8 Å, Cryo-EM structure of the apo hTAAR1-Gs complex
7WVV 2.9 Å, Cryo-EM structure of the human formyl peptide receptor 2 in complex with fMLFII and Gi2
9DQJ 2.9 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
9KFI 2.91 Å, Cryo-EM structure of the human relaxin family peptide receptor 3 in complex with…
9KFK 2.95 Å, Cryo-EM structure of the relaxin-3-bound human relaxin family peptide receptor 4…

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