7FIG: PDB entry 7FIG

luteinizing hormone/choriogonadotropin receptor(S277I)-chorionic gonadotropin-Gs complex. Determined by electron microscopy at 3.9 Å resolution. Released 29 Sept 2021.

Method: Electron microscopy
Resolution: 3.9 Å
Organisms: Bos taurus, Rattus norvegicus, Lama glama
Chains: 7
Atoms: 12,839
Mol. weight: 213.68 kDa
Ligands: NAG
Released: 29 Sept 2021

Explore 7FIG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7FIG contains 53 α-helices and 91 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	33	1	1
β-strand	36-39	4	2
α-helix	42-44	3
α-helix	46-54	9
α-helix	63-90	28
α-helix	100-112	13
α-helix	121-131	11
α-helix	134-142	9
α-helix	152-162	11
α-helix	171-178	8
β-strand	183-186	4	2
β-strand	189-191	3	1
β-strand	194-196	3	1
β-strand	198-202	5	2
α-helix	211-217	7
β-strand	220-226	7	2
α-helix	233-245	13
α-helix	247-249	3
β-strand	254-259	6	2
α-helix	261-269	9
α-helix	275-278	4
α-helix	280-283	4
α-helix	293-294	2
α-helix	299-318	20
β-strand	326-330	5	2
α-helix	338-357	20

Chain B: 5 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-42	5
β-strand	43	1	3
β-strand	49-50	2	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	91-94	4	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	122-125	4	6
β-strand	135-138	4	6
β-strand	149-153	5	7
β-strand	156-159	4	7
β-strand	166-170	5	7
β-strand	178-180	3	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	218-222	5	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
α-helix	272	1
β-strand	273-278	6	3
β-strand	284-289	6	3
β-strand	294-298	5	3
β-strand	306-308	3	3
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-339	4	4

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8-24	17
α-helix	30-44	15

Chain N: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	10-12	3	11
β-strand	18-25	8	10
α-helix	29-31	3
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	68-73	6	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	92-98	7	11
β-strand	118	1	11
β-strand	122-126	5	11

Chain R: 23 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	53-56	4	12
β-strand	63-64	2	13
β-strand	70	1	14
β-strand	72	1	14
β-strand	77-80	4	12
β-strand	88-89	2	13
β-strand	94-95	2	15
β-strand	102-108	7	12
β-strand	113-114	2	13
β-strand	119-120	2	15
β-strand	127-133	7	12
α-helix	142-144	3
β-strand	146	1	16
β-strand	151-156	6	12
β-strand	163-164	2	17
α-helix	165	1
β-strand	173	1	16
β-strand	177-180	4	12
β-strand	188-189	2	17
β-strand	195-198	4	18
β-strand	199-203	5	12
β-strand	212-213	2	17
α-helix	217-219	3
β-strand	220-222	3	18
β-strand	226-228	3	12
α-helix	242-244	3
β-strand	247-249	3	12
β-strand	250	1	19
α-helix	259-261	3
α-helix	262-265	4
β-strand	270	1	12
β-strand	273	1	19
α-helix	276-278	3
α-helix	279-282	4
α-helix	334-336	3
β-strand	342	1	12
α-helix	346-348	3
α-helix	360-386	27
α-helix	393-422	30
α-helix	426-435	10
α-helix	437-469	33
α-helix	480-499	20
α-helix	508-510	3
α-helix	522-553	32
α-helix	561-593	33
α-helix	604-606	3
α-helix	607-611	5
α-helix	612-620	9
α-helix	621-625	5
α-helix	628-640	13

Chain X: 2 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	8-10	3
β-strand	11-14	4	20
β-strand	20	1	21
β-strand	23	1	21
β-strand	26-38	13	20
α-helix	41-44	4
β-strand	53-57	5	20
β-strand	59-69	11	22
β-strand	75-85	11	22
β-strand	91	1	23

Chain Y: 1 helix, 7 β-strands

Element	Residues	Length	Sheet
β-strand	8	1	20
β-strand	10-18	9	20
β-strand	27-41	15	20
β-strand	56-68	13	24
α-helix	72-73	2
β-strand	79-91	13	24
β-strand	93	1	23
β-strand	98-101	4	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Engineered guanine nucleotide-binding protein G(s) subunit alpha	A	protein	361	Bos taurus
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Camelid antibody VHH fragment Nb35	N	protein	134	Lama glama
Lutropin-choriogonadotropic hormone receptor	R	protein	697	Homo sapiens	P22888 (AlphaFold model)
Glycoprotein hormones alpha chain	X	protein	116	Homo sapiens	P01215 (AlphaFold model)
Choriogonadotropin subunit beta 3	Y	protein	165	Homo sapiens	P0DN86

Sequence of entity 1 (A), FASTA

>7FIG_1 Engineered guanine nucleotide-binding protein G(s) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B), FASTA

>7FIG_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7FIG_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>7FIG_4 Camelid antibody VHH fragment Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHH

Sequence of entity 5 (R), FASTA

>7FIG_5 Lutropin-choriogonadotropic hormone receptor (chains R)
DYKDDDDVENLYFQGASALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFR
GLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSI
CNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQ
SHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIA
TSSYSLKKLPSRETFVNLLEATLTYPIHCCAFRNLPTKEQNFSHSISENFSKQCESTVRK
VNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLRVLIWLIN
ILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHA
IDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGG
WLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIY
FAVRNPELMATNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVL
FYPINSCANPFLYAIFTKTFQRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGS
NKPSQSTLKLSTLHCQGTALLDKTRYTECHHHHHHHH

Sequence of entity 6 (X), FASTA

>7FIG_6 Glycoprotein hormones alpha chain (chains X)
MDYYRKYAAIFLVTLSVFLHVLHSAPDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRA
YPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

Sequence of entity 7 (Y), FASTA

>7FIG_7 Choriogonadotropin subunit beta 3 (chains Y)
MEMFQGLLLLLLLSMGGTWASKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPT
MTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDC
GGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	5

Primary citation

Structures of full-length glycoprotein hormone receptor signalling complexes. Duan, J., Xu, P., Cheng, X. et al. Nature (2021) 598:688-692. DOI 10.1038/s41586-021-03924-2 · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF

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