7FIH: PDB entry 7FIH

luteinizing hormone/choriogonadotropin receptor(S277I)-chorionic gonadotropin-Gs-Org43553 complex. Determined by electron microscopy at 3.2 Å resolution. Released 29 Sept 2021.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Bos taurus, Rattus norvegicus, Homo sapiens
Chains: 6
Atoms: 10,853
Mol. weight: 198.24 kDa
Ligands: 55Z
Released: 29 Sept 2021

Explore 7FIH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7FIH contains 41 α-helices and 76 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	14-37	24
β-strand	40-46	7	1
α-helix	53-61	9
β-strand	213-214	2	1
β-strand	217-223	7	1
α-helix	235-238	4
β-strand	243-249	7	1
α-helix	255-267	13
β-strand	277-282	6	1
α-helix	284-292	9
α-helix	298-301	4
α-helix	303-305	3
α-helix	316-317	2
α-helix	322-341	20
β-strand	349-353	5	1
α-helix	361-380	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-40	3
β-strand	47-51	5	2
β-strand	60-63	4	3
β-strand	69-73	5	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	102-103	2	4
β-strand	111-115	5	4
β-strand	121-125	5	4
β-strand	135-139	5	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	306-308	3	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-23	17
α-helix	30-43	14

Chain R: 21 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	53-57	5	9
β-strand	63-64	2	10
α-helix	65	1
β-strand	77-81	5	9
β-strand	88-89	2	10
β-strand	94	1	11
β-strand	102-105	4	9
β-strand	108	1	12
β-strand	113-114	2	10
α-helix	115	1
β-strand	119	1	11
β-strand	127-131	5	9
β-strand	133	1	12
α-helix	138-140	3
β-strand	146	1	13
β-strand	151-156	6	9
β-strand	163-164	2	14
β-strand	173	1	13
β-strand	177-180	4	9
β-strand	188-189	2	14
β-strand	195-198	4	15
β-strand	199-203	5	9
β-strand	212-213	2	14
β-strand	220-222	3	15
β-strand	226-228	3	9
α-helix	242-244	3
β-strand	247-249	3	9
α-helix	262-264	3
β-strand	271-272	2	9
α-helix	276-282	7
β-strand	343-344	2	9
α-helix	346-348	3
α-helix	360-372	13
α-helix	375-385	11
α-helix	390-392	3
α-helix	393-421	29
α-helix	426-434	9
α-helix	437-469	33
α-helix	480-497	18
α-helix	500-503	4
α-helix	522-553	32
α-helix	564-593	30
α-helix	601-611	11
α-helix	614-619	6
α-helix	620-625	6
α-helix	628-641	14

Chain X: 2 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	16
β-strand	11-14	4	17
β-strand	20	1	18
β-strand	23	1	18
β-strand	26-29	4	17
β-strand	30-38	9	16
α-helix	39-40	2
α-helix	41-44	4
β-strand	53-57	5	16
β-strand	59-69	11	19
β-strand	75-85	11	19
β-strand	91	1	20

Chain Y: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7	1
β-strand	8	1	16
β-strand	10-18	9	16
β-strand	27-40	14	16
α-helix	50-53	4
β-strand	56-68	13	21
α-helix	72-73	2
β-strand	79-91	13	21
β-strand	93	1	20
β-strand	99-101	3	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Engineered Guanine nucleotide-binding protein G(s) subunit alpha	A	protein	361	Bos taurus
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Lutropin-choriogonadotropic hormone receptor	R	protein	697	Homo sapiens	P22888 (AlphaFold model)
Glycoprotein hormones alpha chain	X	protein	115	Homo sapiens	P01215 (AlphaFold model)
Choriogonadotropin subunit beta 3	Y	protein	165	Homo sapiens	P0DN86

Sequence of entity 1 (A), FASTA

>7FIH_1 Engineered Guanine nucleotide-binding protein G(s) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B), FASTA

>7FIH_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7FIH_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (R), FASTA

>7FIH_4 Lutropin-choriogonadotropic hormone receptor (chains R)
DYKDDDDVENLYFQGASALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFR
GLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSI
CNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQ
SHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIA
TSSYSLKKLPSRETFVNLLEATLTYPIHCCAFRNLPTKEQNFSHSISENFSKQCESTVRK
VNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLRVLIWLIN
ILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHA
IDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGG
WLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIY
FAVRNPELMATNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVL
FYPINSCANPFLYAIFTKTFQRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGS
NKPSQSTLKLSTLHCQGTALLDKTRYTECHHHHHHHH

Sequence of entity 5 (X), FASTA

>7FIH_5 Glycoprotein hormones alpha chain (chains X)
DYYRKYAAIFLVTLSVFLHVLHSAPDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAY
PTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

Sequence of entity 6 (Y), FASTA

>7FIH_6 Choriogonadotropin subunit beta 3 (chains Y)
MEMFQGLLLLLLLSMGGTWASKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPT
MTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDC
GGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ

Ligands and cofactors

ID	Name	Formula	Copies
55Z	5-azanyl-N-tert-butyl-2-methylsulfanyl-4-[3-(2-morpholin-4-ylethanoylamino)phen…	C24 H30 N6 O3 S2	1

Primary citation

Structures of full-length glycoprotein hormone receptor signalling complexes. Duan, J., Xu, P., Cheng, X. et al. Nature (2021) 598:688-692. DOI 10.1038/s41586-021-03924-2 · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF

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