7FII: PDB entry 7FII

luteinizing hormone/choriogonadotropin receptor-chorionic gonadotropin-Gs complex. Determined by electron microscopy at 4.3 Å resolution. Released 29 Sept 2021.

Method: Electron microscopy
Resolution: 4.3 Å
Organisms: Bos taurus, Rattus norvegicus, Homo sapiens
Chains: 6
Atoms: 8,765
Mol. weight: 197.83 kDa
Released: 29 Sept 2021

Explore 7FII in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7FII contains 48 α-helices and 84 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-34	2	1
α-helix	35	1
β-strand	36-40	5	1
α-helix	42-44	3
α-helix	46-54	9
β-strand	57	1	1
α-helix	63-90	28
α-helix	100-112	13
α-helix	118-120	3
α-helix	121-132	12
α-helix	134-142	9
α-helix	152-162	11
α-helix	171-178	8
β-strand	184-190	7	1
β-strand	195-201	7	1
α-helix	208-211	4
α-helix	212-217	6
β-strand	220-226	7	1
α-helix	232-244	13
β-strand	254-259	6	1
α-helix	261-269	9
α-helix	275-278	4
α-helix	280-282	3
α-helix	293-294	2
α-helix	299-316	18
β-strand	326-330	5	1
α-helix	338-357	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-34	5
α-helix	38-40	3
β-strand	47-51	5	2
β-strand	58-62	5	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	102-103	2	4
β-strand	111-115	5	4
β-strand	122-125	4	4
β-strand	135-138	4	4
β-strand	146-150	5	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-179	4	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-23	17
α-helix	30-45	16

Chain R: 17 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	53-54	2	9
β-strand	63-64	2	10
β-strand	77-80	4	9
β-strand	83	1	11
β-strand	88-89	2	10
β-strand	94-95	2	12
β-strand	102-105	4	9
β-strand	108	1	11
β-strand	113-114	2	10
β-strand	119-120	2	12
β-strand	127-130	4	9
α-helix	138-140	3
β-strand	146	1	13
β-strand	151-155	5	9
β-strand	158	1	14
β-strand	164	1	15
α-helix	165	1
β-strand	173	1	13
β-strand	177-180	4	9
β-strand	184	1	14
β-strand	189	1	15
β-strand	195-197	3	16
β-strand	199-203	5	9
β-strand	220-221	2	16
β-strand	226-228	3	9
β-strand	247-250	4	9
α-helix	262-264	3
β-strand	270-273	4	9
α-helix	276-279	4
β-strand	342-344	3	9
α-helix	346-348	3
α-helix	362-385	24
α-helix	390-391	2
α-helix	393-421	29
α-helix	426-435	10
α-helix	437-469	33
α-helix	480-503	24
β-strand	511	1	17
β-strand	515	1	17
α-helix	522-553	32
α-helix	561-593	33
α-helix	601-610	10
α-helix	614-620	7
α-helix	621-625	5
α-helix	628-641	14

Chain X: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	11-14	4	18
β-strand	20	1	19
β-strand	23	1	19
α-helix	24-25	2
β-strand	26-30	5	18
β-strand	34	1	20
β-strand	35-37	3	21
α-helix	41-45	5
β-strand	54-56	3	21
β-strand	60-62	3	22
β-strand	65-69	5	23
α-helix	71-73	3
β-strand	75-79	5	23
β-strand	82-84	3	22

Chain Y: 3 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	8	1	24
β-strand	10-18	9	25
β-strand	27-35	9	25
β-strand	36	1	20
β-strand	37	1	24
β-strand	40-41	2	18
α-helix	50-53	4
β-strand	56-68	13	26
α-helix	72-73	2
β-strand	79-91	13	26
α-helix	92-94	3
β-strand	101	1	21

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Engineered Guanine nucleotide-binding protein G(s) subunit alpha	A	protein	361	Bos taurus
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Lutropin-choriogonadotropic hormone receptor	R	protein	697	Homo sapiens	P22888 (AlphaFold model)
Glycoprotein hormones alpha chain	X	protein	116	Homo sapiens	P01215 (AlphaFold model)
Choriogonadotropin subunit beta 3	Y	protein	165	Homo sapiens	P0DN86

Sequence of entity 1 (A), FASTA

>7FII_1 Engineered Guanine nucleotide-binding protein G(s) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B), FASTA

>7FII_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7FII_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (R), FASTA

>7FII_4 Lutropin-choriogonadotropic hormone receptor (chains R)
DYKDDDDVENLYFQGASALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFR
GLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSI
CNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQ
SHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIA
TSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRK
VNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLRVLIWLIN
ILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHA
IDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGG
WLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIY
FAVRNPELMATNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVL
FYPINSCANPFLYAIFTKTFQRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGS
NKPSQSTLKLSTLHCQGTALLDKTRYTECHHHHHHHH

Sequence of entity 5 (X), FASTA

>7FII_5 Glycoprotein hormones alpha chain (chains X)
MDYYRKYAAIFLVTLSVFLHVLHSAPDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRA
YPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

Sequence of entity 6 (Y), FASTA

>7FII_6 Choriogonadotropin subunit beta 3 (chains Y)
MEMFQGLLLLLLLSMGGTWASKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPT
MTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDC
GGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ

Primary citation

Structures of full-length glycoprotein hormone receptor signalling complexes. Duan, J., Xu, P., Cheng, X. et al. Nature (2021) 598:688-692. DOI 10.1038/s41586-021-03924-2 · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF

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