7LLL: PDB entry 7LLL

Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein. Determined by electron microscopy at 3.7 Å resolution. Released 12 Jan 2022.

Method: Electron microscopy
Resolution: 3.7 Å
Organisms: Homo sapiens, Lama glama, Heloderma suspectum
Chains: 6
Atoms: 8,940
Mol. weight: 164.3 kDa
Released: 12 Jan 2022

Explore 7LLL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7LLL contains 46 α-helices and 58 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	12-22	11
α-helix	24-32	9
β-strand	40-47	8	1
β-strand	206	1	2
β-strand	209-214	6	1
β-strand	217-222	6	1
β-strand	225	1	2
β-strand	243-249	7	1
α-helix	267-277	11
β-strand	286-292	7	1
α-helix	294-301	8
α-helix	313-315	3
α-helix	332-349	18
β-strand	359	1	1
α-helix	371-374	4
α-helix	376-379	4
α-helix	381-390	10

Chain B: 3 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	2-22	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	51	1	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	91-94	4	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	135-139	5	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	166-168	3	6
β-strand	178-180	3	6
β-strand	187-192	6	7
β-strand	199-203	5	7
β-strand	207-210	4	7
β-strand	220-222	3	7
β-strand	229-234	6	8
β-strand	241-245	5	8
β-strand	250-253	4	8
β-strand	260-264	5	8
β-strand	273-278	6	9
β-strand	285-289	5	9
β-strand	295-297	3	10
β-strand	304-307	4	10
β-strand	315-320	6	11
β-strand	327-331	5	11
β-strand	336	1	3
β-strand	337-339	3	11

Chain G: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	8-20	13
α-helix	30-32	3
α-helix	35-43	9
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain N: 2 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	11-12	2	12
β-strand	18	1	13
β-strand	23	1	14
α-helix	29-31	3
β-strand	34-39	6	15
β-strand	45-51	7	15
β-strand	59	1	15
β-strand	70-73	4	14
β-strand	78-81	4	14
β-strand	83	1	13
β-strand	93-95	3	15
β-strand	97	1	16
β-strand	98	1	15
α-helix	99	1
β-strand	118	1	16
β-strand	122-123	2	15
β-strand	125-126	2	12

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-27	26

Chain R: 25 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	32-52	21
α-helix	54-56	3
β-strand	80-81	2	17
β-strand	84	1	18
α-helix	85-86	2
α-helix	90-92	3
β-strand	99	1	18
β-strand	102-103	2	17
β-strand	112	1	19
β-strand	117	1	19
α-helix	139-148	10
α-helix	151-168	18
α-helix	175-203	29
α-helix	224-227	4
α-helix	229-236	8
α-helix	245-250	6
α-helix	258-259	2
α-helix	265-267	3
α-helix	270-273	4
α-helix	277-279	3
α-helix	282-290	9
α-helix	295-297	3
α-helix	305-307	3
α-helix	311-328	18
α-helix	331-337	7
α-helix	346-360	15
α-helix	363-366	4
α-helix	378-390	13
α-helix	394-399	6
α-helix	400-404	5
α-helix	407-418	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	58	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	128	Lama glama
Exendin-4	P	protein	39	Heloderma suspectum	P26349 (AlphaFold model)
Glucagon-like peptide 1 receptor	R	protein	491	Homo sapiens	P43220

Sequence of entity 1 (A), FASTA

>7LLL_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7LLL_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
QSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7LLL_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
NTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR

Sequence of entity 4 (N), FASTA

>7LLL_4 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS

Sequence of entity 5 (P), FASTA

>7LLL_5 Exendin-4 (chains P)
HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS

Sequence of entity 6 (R), FASTA

>7LLL_6 Glucagon-like peptide 1 receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPARPQGATVSLWETVQKWREYRRQCQRSLT
EDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWL
QKDNSSLPWRDLSECEESKRGERSSPEEQLLFLYIIYTVGYALSFSALVIASAILLGFRH
LHCTRNYIHLNLFASFILRALSVFIKDAALKWMYSTAAQQHQWDGLLSYQDSLSCRLVFL
LMQYCVAANYYWLLVEGVYLYTLLAFSVFSEQWIFRLYVSIGWGVPLLFVVPWGIVKYLY
EDEGCWTRNSNMNYWLIIRLPILFAIGVNFLIFVRVICIVVSKLKANLMCKTDIKCRLAK
STLTLIPLLGTHEVIFAFVMDEHARGTLRFIKLFTELSFTSFQGLMVAILYCFVNNEVQL
EFRKSWERWRLEHLHIQRDSSMKPLKCPTSSLSSGATAGSSMYTATCQASCSPAGLEVLF
QGPHHHHHHHH

Primary citation

Dynamics of GLP-1R peptide agonist engagement are correlated with kinetics of G protein activation. Deganutti, G., Liang, Y.L., Zhang, X. et al. Nat Commun (2022) 13:92-92. DOI 10.1038/s41467-021-27760-0 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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