7OAO: Nanobody C5

Nanobody C5 bound to RBD. Determined by X-ray diffraction at 1.5 Å resolution. Released 11 Aug 2021.

Method: X-ray diffraction
Resolution: 1.5 Å
Organisms: Severe acute respiratory syndrome coronavirus 2, Lama glama
Chains: 2
Atoms: 2,800
Mol. weight: 37.83 kDa
Ligands: NAG
Released: 11 Aug 2021

Explore 7OAO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7OAO contains 15 α-helices and 29 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain EEE: 12 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	334	1
β-strand	335	1	1
α-helix	336	1
α-helix	339-342	4
β-strand	349	1	2
α-helix	350-352	3
β-strand	354-358	5	2
β-strand	361-362	2	1
α-helix	365-369	5
β-strand	376-380	5	2
α-helix	384-389	6
β-strand	391-392	2	1
β-strand	394-403	10	2
α-helix	404-409	6
α-helix	417-421	5
β-strand	431-437	7	2
α-helix	439-442	4
β-strand	448	1	3
β-strand	452-454	3	4
α-helix	460-462	3
α-helix	471-472	2
β-strand	473-474	2	5
β-strand	485	1	5
β-strand	488-489	2	5
β-strand	492-494	3	4
β-strand	497	1	3
α-helix	503-505	3
β-strand	507-516	10	2
β-strand	524-525	2	1

Chain FFF: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	6
β-strand	10-13	4	7
β-strand	18-25	8	6
β-strand	33-39	7	7
β-strand	46-52	7	7
β-strand	58-60	3	7
β-strand	65	1	6
β-strand	68-73	6	6
α-helix	74-76	3
β-strand	78-83	6	6
α-helix	88-90	3
β-strand	92-98	7	7
α-helix	107-108	2
β-strand	110-111	2	7
β-strand	115-120	6	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Spike protein S1	EEE	protein	210	Severe acute respiratory syndrome coronavirus 2	P0DTC2 (AlphaFold model)
C5 nanobody	FFF	protein	128	Lama glama

Sequence of entity 1 (EEE), FASTA

>7OAO_1 Spike protein S1 (chains EEE)
PNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLND
LCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNY
NYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
VVVLSFELLHAPATVCGPKKSTNKHHHHHH

Sequence of entity 2 (FFF), FASTA

>7OAO_2 C5 nanobody (chains FFF)
QVQLVESGGGSVQAGGSLTLSCVASGVTLGRHAIGWFRQAPGKERERVSCIRTFDGITSY
VESTKGRFTISSNNAMNTVYLQMNSLKPEDTAVYFCALGVTAACSDNPYFWGQGTQVTVS
SKHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1

Water and common crystallization additives (ACT) are not listed.

Primary citation

A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19. Huo, J., Mikolajek, H., Le Bas, A. et al. Nat Commun (2021) 12:5469-5469. DOI 10.1038/s41467-021-25480-z · PubMed

Other PDB entries of the same protein (UniProt P0DTC2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7Z8O 0.96 Å, Crystal structure of SARS-CoV-2 S RBD in complex with a stapled peptide
7Y8J 1.03 Å, 3D1 in complex with 6-mer HR1 peptide from SARS-CoV-2
9FC2 1.21 Å, The crystal structure of the SARS-CoV-2 receptor binding domain in complex with the…
7EK6 1.24 Å, Structure of viral peptides IPB19/N52
7EAM 1.4 Å, immune complex of SARS-CoV-2 RBD and cross-neutralizing antibody 7D6
7M53 1.4 Å, B6 Fab fragment bound to the SARS-CoV/SARS-CoV-2 spike stem helix peptide
8CMC 1.42 Å, Human Leukocyte Antigen class II allotype DR1 presenting SARS-CoV-2 Spike peptide S511-530
7M8U 1.45 Å, Crystal Structure of HLA-B*35:01 in complex with IPFAMQMAY, an 9-mer epitope from…
7ZR2 1.45 Å, Crystal structure of a chimeric protein mimic of SARS-CoV-2 Spike HR1 in complex with HR2
8D36 1.45 Å, Crystal structure of SARS-CoV-2 fusion peptide in complex with neutralizing antibody…
8ENS 1.45 Å, Crystal structure of beta'-COPI-WD40 domain in complex with SARS-CoV-2 spike tail…
8ENW 1.45 Å, Crystal structure of beta'-COPI-WD40 domain in complex with SARS-CoV-2 clientized spike…

Browse structure collections

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