7OWG: Serine/threonine-protein kinase mTOR

human DEPTOR in a complex with mutant human mTORC1 A1459P. Determined by electron microscopy at 4.7 Å resolution. Released 8 Sept 2021.

Method: Electron microscopy
Resolution: 4.7 Å
Organism: Homo sapiens
Chains: 4
Atoms: 28,537
Mol. weight: 519.05 kDa
Released: 8 Sept 2021

Explore 7OWG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7OWG contains 206 α-helices and 91 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 150 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	21-24	4
α-helix	38-40	3
α-helix	41-50	10
α-helix	61-64	4
α-helix	67-72	6
α-helix	83-97	15
α-helix	104-113	10
α-helix	115-117	3
α-helix	123-134	12
α-helix	142-154	13
α-helix	167-178	12
α-helix	182-186	5
α-helix	187-192	6
α-helix	193-196	4
α-helix	203	1
α-helix	204-208	5
α-helix	214-222	9
α-helix	234-239	6
α-helix	240-244	5
α-helix	267-274	8
α-helix	282-286	5
α-helix	362-373	12
α-helix	374-376	3
α-helix	387-402	16
α-helix	412-420	9
α-helix	436-441	6
α-helix	448-450	3
α-helix	452-454	3
α-helix	456-462	7
α-helix	479-490	12
α-helix	498-501	4
α-helix	502-505	4
α-helix	507-511	5
α-helix	515-527	13
α-helix	529-548	20
α-helix	579-589	11
α-helix	600-610	11
α-helix	616-630	15
α-helix	646-665	20
α-helix	669-677	9
α-helix	681-683	3
α-helix	684-687	4
α-helix	690-700	11
α-helix	705-718	14
α-helix	723-743	21
α-helix	747-761	15
α-helix	765-767	3
α-helix	769-771	3
α-helix	772-784	13
α-helix	793-809	17
α-helix	810-816	7
α-helix	817-829	13
α-helix	837-851	15
α-helix	857-860	4
α-helix	864-874	11
α-helix	878-891	14
α-helix	896-901	6
α-helix	934-940	7
α-helix	943-959	17
α-helix	964-980	17
α-helix	983-985	3
α-helix	987-989	3
α-helix	990-1003	14
α-helix	1006-1023	18
α-helix	1024-1030	7
α-helix	1031-1040	10
α-helix	1046-1062	17
α-helix	1066-1069	4
α-helix	1071-1082	12
α-helix	1088-1090	3
α-helix	1091-1100	10
α-helix	1104-1110	7
α-helix	1111-1123	13
α-helix	1129-1145	17
α-helix	1149-1151	3
α-helix	1152-1165	14
α-helix	1172-1184	13
α-helix	1185-1191	7
α-helix	1192-1201	10
α-helix	1207-1217	11
α-helix	1263-1269	7
α-helix	1270-1273	4
α-helix	1277-1293	17
α-helix	1298-1309	12
α-helix	1311-1326	16
α-helix	1331-1347	17
α-helix	1351-1366	16
α-helix	1371-1373	3
α-helix	1377-1379	3
α-helix	1380-1389	10
α-helix	1393-1405	13
α-helix	1410-1422	13
α-helix	1426-1438	13
α-helix	1444-1449	6
α-helix	1478-1487	10
α-helix	1490-1503	14
α-helix	1507-1522	16
α-helix	1526-1533	8
α-helix	1541-1553	13
α-helix	1557-1577	21
α-helix	1587-1590	4
α-helix	1593-1607	15
α-helix	1609-1611	3
α-helix	1612-1623	12
α-helix	1630-1640	11
α-helix	1650-1658	9
α-helix	1666-1677	12
α-helix	1681-1683	3
α-helix	1688-1690	3
α-helix	1694-1705	12
α-helix	1710-1729	20
α-helix	1737-1762	26
α-helix	1768-1782	15
α-helix	1787-1806	20
α-helix	1807-1811	5
α-helix	1868-1894	27
α-helix	1900-1913	14
α-helix	1917-1927	11
α-helix	1933-1936	4
α-helix	1939-1943	5
α-helix	1951-1967	17
α-helix	1969-1978	10
α-helix	1979-1981	3
α-helix	1985-2001	17
α-helix	2005-2020	16
α-helix	2023-2038	16
α-helix	2043-2045	3
α-helix	2047-2051	5
α-helix	2052-2059	8
α-helix	2068-2091	24
α-helix	2094-2114	21
β-strand	2120-2122	3	1
α-helix	2123-2126	4
β-strand	2139	1	2
β-strand	2152	1	2
β-strand	2153-2156	4	1
β-strand	2159-2162	4	1
β-strand	2170-2176	7	1
β-strand	2181-2188	8	1
α-helix	2193-2211	19
α-helix	2213-2217	5
β-strand	2227-2229	3	1
β-strand	2234-2238	5	1
β-strand	2243-2245	3	3
α-helix	2246-2257	12
α-helix	2263-2271	9
α-helix	2275-2277	3
α-helix	2280-2292	13
α-helix	2298-2306	9
α-helix	2310-2334	25
α-helix	2341-2343	3
β-strand	2344-2347	4	3
β-strand	2353-2355	3	3
α-helix	2364-2367	4
α-helix	2381-2386	6
α-helix	2395-2409	15
α-helix	2411-2423	13
α-helix	2427-2434	8
α-helix	2494-2509	16
α-helix	2521-2531	11
α-helix	2535-2538	4
α-helix	2543-2545	3

Chain E: 4 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	14-19	6	4
β-strand	24-28	5	4
α-helix	29-31	3
β-strand	36-38	3	4
β-strand	45-50	6	5
β-strand	56-61	6	5
β-strand	64-69	6	5
β-strand	79-82	4	5
β-strand	90-94	5	6
β-strand	99-103	5	6
β-strand	108-113	6	6
β-strand	121-126	6	6
α-helix	130	1
β-strand	131-135	5	7
β-strand	142-147	6	7
β-strand	152	1	8
β-strand	153-156	4	7
β-strand	162	1	7
β-strand	165	1	8
β-strand	173-178	6	9
β-strand	184-189	6	9
β-strand	194-200	7	9
β-strand	209-216	8	9
α-helix	222	1
β-strand	223-228	6	10
β-strand	234-239	6	10
β-strand	243-248	6	10
β-strand	253-259	7	10
α-helix	265-267	3
β-strand	276-279	4	11
β-strand	284-287	4	11
β-strand	294-298	5	11
β-strand	304-308	5	11
β-strand	315-321	7	4

Chain O: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	327-334	8	12
β-strand	336	1	13
β-strand	339	1	13
β-strand	342-346	5	12
β-strand	351-355	5	12
α-helix	360-364	5
α-helix	370	1
β-strand	371-375	5	12
β-strand	378-379	2	12
α-helix	385-391	7
β-strand	398-405	8	12

Chain Y: 49 helices, 41 β-strands

Element	Residues	Length	Sheet
α-helix	19-25	7
α-helix	31-34	4
β-strand	57-60	4	14
β-strand	63-65	3	14
β-strand	85	1	15
β-strand	88	1	15
α-helix	95-113	19
β-strand	118-119	2	14
β-strand	122-123	2	14
α-helix	127-140	14
β-strand	145-152	8	14
α-helix	156-159	4
β-strand	164	1	16
β-strand	175	1	16
α-helix	179-185	7
β-strand	190-195	6	14
α-helix	199-217	19
β-strand	241-244	4	14
α-helix	262-268	7
α-helix	270-281	12
α-helix	292-295	4
α-helix	308-327	20
α-helix	330-337	8
α-helix	341-359	19
β-strand	365-366	2	14
α-helix	370-372	3
α-helix	378-390	13
α-helix	393-396	4
α-helix	403-406	4
α-helix	408-422	15
α-helix	433-441	9
α-helix	446-457	12
α-helix	461-470	10
α-helix	473-479	7
α-helix	485-487	3
α-helix	488-492	5
α-helix	493-501	9
α-helix	508-511	4
α-helix	516-521	6
α-helix	529-545	17
α-helix	548-554	7
α-helix	558-564	7
α-helix	565-567	3
α-helix	571-584	14
α-helix	589-597	9
α-helix	600-603	4
α-helix	604-607	4
α-helix	613-628	16
α-helix	636-648	13
β-strand	654	1	17
α-helix	657-674	18
α-helix	678-681	4
α-helix	682-685	4
α-helix	807-818	12
α-helix	824-838	15
α-helix	959-967	9
α-helix	974-975	2
α-helix	983-987	5
α-helix	988-992	5
α-helix	993	1
α-helix	995-1007	13
β-strand	1015-1021	7	18
β-strand	1029-1031	3	19
β-strand	1037-1040	4	19
β-strand	1045-1050	6	19
β-strand	1055-1061	7	19
β-strand	1070-1075	6	20
β-strand	1084-1088	5	20
β-strand	1093-1097	5	20
α-helix	1099-1101	3
β-strand	1107-1112	6	20
β-strand	1128-1132	5	17
β-strand	1137-1142	6	17
β-strand	1146-1151	6	17
β-strand	1156-1162	7	17
β-strand	1169-1175	7	21
β-strand	1180-1185	6	21
β-strand	1190-1193	4	21
β-strand	1203-1207	5	21
β-strand	1214-1219	6	22
β-strand	1227-1231	5	22
β-strand	1235-1239	5	22
β-strand	1246-1248	3	22
β-strand	1259-1261	3	23
β-strand	1267-1270	4	23
β-strand	1276-1281	6	23
β-strand	1286-1291	6	23
β-strand	1306-1309	4	18
β-strand	1316-1320	5	18
β-strand	1324-1329	6	18

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Serine/threonine-protein kinase mTOR	B	protein	2549	Homo sapiens	P42345 (AlphaFold model)
Target of rapamycin complex subunit LST8	E	protein	326	Homo sapiens	Q9BVC4 (AlphaFold model)
DEP domain-containing mTOR-interacting protein	O	protein	409	Homo sapiens	Q8TB45 (AlphaFold model)
Regulatory-associated protein of mTOR	Y	protein	1335	Homo sapiens	Q8N122 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>7OWG_1 Serine/threonine-protein kinase mTOR (chains B)
MLGTGPAAATTAATTSXXXXXXXXXXXXXXSRNEETXXXXXXXXXXXXXXXXXEMSQEEX
TRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDP
VVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVP
TFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEE
AEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
KDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTXXXXX
XXXXXXXXXXXXXXXXXXKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHV
LSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDA
TVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLL
KMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLT
QFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLL
VVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSS
MNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALI
LKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVA
LWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHK
VNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFR
DQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLV
SFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLR
VFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVD
RLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLV
RHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHV
STINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNA
AFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGI
VLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHF
GELEIQATWYEKLHEWEDPLVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEK
WTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLA
QQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
ERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVD
PSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHK
QELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEA
VLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSP
TPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVN
EALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVAS
KSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFG
ERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQA
WDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSL
QVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNL
SIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTL
MQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRH
PSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITC
HTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDG
VELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLD
VPTQVELLIKQATSHENLCQCYIGWCPFW

Sequence of entity 2 (E), FASTA

>7OWG_2 Target of rapamycin complex subunit LST8 (chains E)
MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAA
GYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQ
CQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDP
DASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSA
DQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVE
TGEIKREYGGHQKAVVCLAFNDSVLG

Sequence of entity 3 (O), FASTA

>7OWG_3 DEP domain-containing mTOR-interacting protein (chains O)
MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTY
PNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKD
DGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEA
TTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQE
THDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSP
PVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGP
AAAAGMKVCQFVVSVNGLNVLHVDYRTVNNLILTGPRTIVMEVMEELEC

Sequence of entity 4 (Y), FASTA

>7OWG_4 Regulatory-associated protein of mTOR (chains Y)
MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSV
ALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYK
QSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIY
DLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNC
IQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPG
RLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSY
NCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWL
TMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLL
QSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILA
VIVNSYHTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSA
HEKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLVSDGSPMVR
KELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTEGGSLTPVRDSPCTPRLRSV
SSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLGSPENEEHILS
FETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYK
ATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRD
LPSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFC
DWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLN
RNPGVPSVVKFHPFTPCIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQD
CSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMS
SGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALS
ECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALD
IHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGS
NDYYISVYSVEKRVR

Primary citation

Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace. Heimhalt, M., Berndt, A., Wagstaff, J. et al. Elife (2021) 10. DOI 10.7554/eLife.68799 · PubMed

Other PDB entries of the same protein (UniProt P42345 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4DRI 1.45 Å, Co-crystal structure of the PPIase domain of FKBP51, Rapamycin and the FRB fragment of…
9DBO 1.55 Å, Crystal structure of a synthetic Fab (R3E9) in complex with the FRB domain of mTOR
5GPG 1.67 Å, Co-crystal structure of the FK506 binding domain of human FKBP25, Rapamycin and the FRB…
5WBH 1.75 Å, Structure of the FRB domain of mTOR bound to a substrate recruitment peptide of S6K1
4DRJ 1.8 Å, o-crystal structure of the PPIase domain of FKBP52, Rapamycin and the FRB fragment of mTOR
3FAP 1.85 Å, Atomic structures of the rapamycin analogs in complex with both human FKBP12 and frb…
8PPZ 1.85 Å, Co-crystal structure of FKBP12, compound 7 and the FRB fragment of mTOR
8XI9 1.85 Å, Crystal structure of FRB-FKBP fusion protein in complex with rapamycin
9PIW 1.9 Å, Crystal structure of a synthetic Fab (1A) in complex with the FRB domain of mTOR
9DL0 2.0 Å, Crystal structure of a synthetic Fab (R3H8) in complex with the FRB domain of mTOR
9PJ1 2.05 Å, Crystal structure of a synthetic Fab (4R) in complex with the ternary assembly of…
9PIU 2.18 Å, Crystal structure of a synthetic Fab (2C) in complex with the FRB domain of mTOR

Browse structure collections

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