Structure of Hedgehog acyltransferase (HHAT) in complex with megabody 177 bound to IMP-1575. Determined by electron microscopy at 3.59 Å resolution. Released 26 Jan 2022.
Explore 7Q6Z in 3D Show helices and sheets RCSB PDB PDBe
7Q6Z contains 32 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 4-31 | 28 | |
| α-helix | 33-40 | 8 | |
| β-strand | 43-44 | 2 | 1 |
| β-strand | 52-53 | 2 | 1 |
| α-helix | 59-66 | 8 | |
| α-helix | 67-71 | 5 | |
| α-helix | 72-88 | 17 | |
| α-helix | 90-92 | 3 | |
| α-helix | 93-109 | 17 | |
| α-helix | 111-129 | 19 | |
| α-helix | 133-148 | 16 | |
| α-helix | 150-159 | 10 | |
| α-helix | 163-187 | 25 | |
| α-helix | 201-208 | 8 | |
| α-helix | 211-213 | 3 | |
| α-helix | 222-229 | 8 | |
| α-helix | 237-264 | 28 | |
| α-helix | 267-272 | 6 | |
| α-helix | 274-277 | 4 | |
| α-helix | 282-313 | 32 | |
| α-helix | 318-323 | 6 | |
| α-helix | 331-336 | 6 | |
| α-helix | 340-346 | 7 | |
| α-helix | 347-351 | 5 | |
| α-helix | 352-354 | 3 | |
| α-helix | 360-379 | 20 | |
| α-helix | 383-407 | 25 | |
| α-helix | 409-418 | 10 | |
| α-helix | 421-448 | 28 | |
| α-helix | 450-456 | 7 | |
| α-helix | 457-462 | 6 | |
| α-helix | 468-487 | 20 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 26-30 | 5 | 2 |
| β-strand | 812-819 | 8 | 2 |
| β-strand | 827-833 | 7 | 3 |
| β-strand | 840-845 | 6 | 3 |
| β-strand | 851-853 | 3 | 3 |
| α-helix | 855-857 | 3 | |
| β-strand | 862-866 | 5 | 2 |
| β-strand | 871-876 | 6 | 2 |
| α-helix | 881-883 | 3 | |
| β-strand | 885-892 | 8 | 3 |
| β-strand | 902-904 | 3 | 3 |
| β-strand | 908-910 | 3 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Protein-cysteine N-palmitoyltransferase HHAT | A | protein | 503 | Homo sapiens | Q5VTY9 (AlphaFold model) |
| Megabody 177 | B | protein | 919 | Escherichia coli K-12 | P42592 (AlphaFold model) |
>7Q6Z_1 Protein-cysteine N-palmitoyltransferase HHAT (chains A) MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEW SFWMEWGKQWLVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLH TTISFCVAQFRSQLLTWLCSLLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYY TNFSLELCWQQLPAASTSYSFPWMLAYVFYYPVLHNGPILSFSEFIKQMQQQEHDSLKAS LCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIPLLETVSCWTLGGLALAQVLFFYVKY LVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPVGGSQHG LLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSLARYFS PQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYS HVGIAWAQTYATDGTETSQVAPA
>7Q6Z_2 Megabody 177 (chains B) MKYLLPTAAAGLLLLAAQPAMAQVQLVESGGGLVKEETQSGLNNYARVVEKGQYDSLEIP AQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLGYSLLQE SVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQFYYDVRIE DKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFNTFVPLGTAAL TNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAKGLTADGPIQENY NPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQASGGGSGGGGSGGGGSGNADNYKNVINRT GAPQYMKDYDYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFD RLTVWQDGKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLD LVWDGELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGE SEYQVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPA FYLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVTG RWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDLIAW NLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWLRNRDH NGNGVPEYGATRDKAHNTESGEMLFTVKKSLRLSCTASGAIFSTYDVSWYRQAPEKPREL VAIITRGGNTHYADTVKGRFTISRDNAKKTVNLQMNSLKPEDTAVYYCHAGVQGAMLGPR NYWGQGTQVTVSSHHHHHH
| ID | Name | Formula | Copies |
|---|---|---|---|
| CLR | Cholesterol | C27 H46 O | 1 |
| HEM | Protoporphyrin IX containing FE | C34 H32 Fe N4 O4 | 1 |
| 9V3 | 2-(2-methylpropylamino)-1-[(4R)-4-(6-methylpyridin-2-yl)-6,7-dihydro-4H-thieno[… | C19 H25 N3 O S | 1 |
| PLM | Palmitic acid | C16 H32 O2 | 1 |
Structure, mechanism, and inhibition of Hedgehog acyltransferase. Coupland, C.E., Andrei, S.A., Ansell, T.B. et al. Mol Cell (2021) 81:5025-5038.e10. DOI 10.1016/j.molcel.2021.11.018 · PubMed
Other PDB entries of the same protein (UniProt Q5VTY9 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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