7QJA: Actin, cytoplasmic 1

Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 1-12, homogeneous dataset). Determined by electron microscopy at 9.2 Å resolution. Released 26 Jan 2022.

Method
Electron microscopy
Resolution
9.2 Å
Organism
Homo sapiens
Chains
37
Atoms
109,569
Mol. weight
2738.54 kDa
Released
26 Jan 2022

Explore 7QJA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7QJA contains 567 α-helices and 293 β-strands across 37 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain a: 5 helices, 1 β-strand

ElementResiduesLengthSheet
α-helix9-2012
α-helix30-3910
β-strand43146
α-helix51-6414
α-helix69-8416
α-helix92-998
Chain A: 31 helices, 6 β-strands
ElementResiduesLengthSheet
α-helix151-16717
α-helix173-1753
α-helix180-1834
α-helix205-2073
α-helix210-22516
β-strand233-235332
α-helix236-2372
α-helix243-2453
β-strand246-248332
α-helix254-27926
α-helix283-2853
α-helix287-31529
α-helix321-3277
α-helix329-34719
α-helix354-3618
α-helix372-39221
α-helix393-3975
β-strand409-411333
β-strand432-434333
α-helix447-46317
α-helix486-50318
α-helix509-51911
α-helix526-5349
α-helix536-5405
α-helix548-56114
α-helix563-5664
β-strand576-577234
β-strand616-617234
α-helix625-6273
α-helix632-66231
α-helix678-69619
α-helix697-7026
α-helix703-71210
α-helix719-73517
α-helix746-76217
α-helix815-84228
α-helix852-8598
Chain b: 3 helices, 1 β-strand
ElementResiduesLengthSheet
α-helix13-3422
α-helix41-5313
β-strand55146
α-helix57-6711
Chain B: 25 helices, 6 β-strands
ElementResiduesLengthSheet
α-helix247-25812
β-strand266-267212
β-strand276-277212
α-helix288-31326
α-helix321-34323
α-helix368-38821
α-helix395-40410
α-helix419-43012
β-strand448-450313
α-helix459-4635
β-strand464-466313
α-helix477-49519
α-helix530-55223
α-helix555-5617
α-helix562-5665
α-helix572-5809
α-helix582-5854
α-helix594-5985
α-helix601-6077
α-helix615-6195
β-strand623-624214
β-strand639-640214
α-helix647-6493
α-helix654-68633
α-helix697-72125
α-helix722-7276
α-helix728-73912
α-helix744-76118
α-helix769-81446
α-helix826-86338
α-helix870-88011
Chain c: 8 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix4-1411
α-helix33-4311
α-helix45-506
α-helix68-7811
α-helix83-9513
α-helix108-1147
α-helix151-18232
α-helix192-1943
Chain C: 31 helices, 8 β-strands
ElementResiduesLengthSheet
α-helix152-16716
α-helix180-1834
α-helix210-22415
β-strand234115
β-strand247115
α-helix256-27722
α-helix289-2924
α-helix297-31519
α-helix321-3277
α-helix330-34516
α-helix353-3586
α-helix371-38212
α-helix384-39411
β-strand409-411316
β-strand432-434316
α-helix441-4433
α-helix447-46216
α-helix473-4753
α-helix487-50519
α-helix509-5179
α-helix526-5349
α-helix536-5405
β-strand542117
α-helix548-56114
α-helix570-5723
β-strand575-577318
β-strand611117
α-helix613-6153
β-strand616-618318
α-helix625-6284
α-helix632-66231
α-helix682-69615
α-helix697-7026
α-helix703-71210
α-helix719-73618
α-helix746-75914
α-helix766-7683
α-helix817-84226
α-helix854-8596
Chain d: 3 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix18-3316
α-helix41-5111
α-helix57-7418
Chain D: 28 helices, 6 β-strands
ElementResiduesLengthSheet
α-helix247-25812
β-strand266-268319
β-strand275-277319
α-helix288-31023
α-helix318-34528
α-helix373-38715
α-helix393-3953
α-helix409-4157
α-helix419-42911
β-strand448-450320
β-strand464-466320
α-helix468-4703
α-helix477-49519
α-helix534-55118
α-helix556-5616
α-helix562-5665
α-helix571-58010
α-helix583-5864
α-helix594-60411
α-helix605-6073
α-helix615-6206
β-strand622121
α-helix6261
α-helix634-6363
β-strand641121
α-helix648-6514
α-helix654-68835
α-helix698-72023
α-helix721-7288
α-helix729-74012
α-helix748-76114
α-helix769-81042
α-helix827-86438
α-helix874-8807

18 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
actin, cytoplasmic 1eprotein375Homo sapiensA0A6I9HGD1 (AlphaFold model)
Gamma-tubulin complex component 5J, lprotein1024Homo sapiensQ96RT8 (AlphaFold model)
Gamma-tubulin complex component 3B, D, F, H, a, f, h, jprotein907Homo sapiensQ96CW5 (AlphaFold model)
Gamma-tubulin complex component 2A, C, E, Gprotein902Homo sapiensQ9BSJ2 (AlphaFold model)
Gamma-tubulin complex component 4I, Kprotein667Homo sapiensQ9UGJ1
Gamma-tubulin complex component 6L, cprotein1819Homo sapiensQ96RT7
Mitotic-spindle organizing protein 1b, d, g, i, k, mprotein82Homo sapiensQ08AG7
Tubulin gamma-1 chainO, P, Q, R, S, T, U, V, W, X, Y, Zprotein451Homo sapiensP23258
Sequence of entity 1 (e), FASTA
>7QJA_1 actin, cytoplasmic 1 (chains e)
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL
AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY
ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS
GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQ
EYDESGPSIVHRKCF
Sequence of entity 2 (J, l), FASTA
>7QJA_2 Gamma-tubulin complex component 5 (chains J, l)
MARHGPPWSRLDAQQERDVRELVRGVAGLQDEADPNFQLALNFAWSNFRFHRFLDVNSHK
IEKTIEGIYEKFVIHSDLSKAASWKRLTEEFLNAPLPSIKEIKTDAHYSILSLLLCLSDS
PSNSSYVETPRNKEVEKKDDFDWGKYLMEDEEMDIGPYMDTPNWSEESEEENDQQPLSRE
DSGIQVDRTPLEEQDQNRKLDPCISWKDEPDDRSWLEHHVVHQYWTARPSQFPHSLHLHS
NLAAVWDQHLYSSDPLYVPDDRVLVTETQVIRETLWLLSGVKKLFIFQLIDGKVTVRNNI
IVTHLTHSCLRSVLEQIAAYGQVVFRLQEFIDEVMGHSSESMLPGSGSVPKKSTEAPFRT
YQAFMWALYKYFISFKEELAEIEKCIINNDTTITLAIVVDKLAPRLSQLKVLHKVFSTGV
AEVPPDTRNVVRASHLLNTLYKAILEYDNVGEASEQTVSLLFSLWVETVRPYLQTVDEWI
VHGHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSVSEKTENEEKMSDNASASSGSDQG
PSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQCAESTTCQAGARDAERKSLYTLFLESV
QSRLRHGEDSTPQVLTEQQATKENLMKMQSIAESHLELDDVHDPLLAINFARMYLEQSDF
HEKFAGGDVCVDRSSESVTCQTFELTLRSCLYPHIDKQYLDCCGNLMQTLKKDYRLVEYL
QAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSIS
FENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDV
LLFGELVSTAEKPRLKEGLIHEQDTVAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNY
IMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNL
ALMFADGWQAGLGTWRMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAG
MEQS
Sequence of entity 3 (B, D, F, H, a, f, h, j), FASTA
>7QJA_3 Gamma-tubulin complex component 3 (chains B, D, F, H, a, f, h, j)
MATPDQKSPNVLLQNLCCRILGRSEADVAQQFQYAVRVIGSNFAPTVERDEFLVAEKIKK
ELIRQRREADAALFSELHRKLHSQGVLKNKWSILYLLLSLSEDPRRQPSKVSSYATLFAQ
ALPRDAHSTPYYYARPQTLPLSYQDRSAQSAQSSGSVGSSGISSIGLCALSGPAPAPQSL
LPGQSNQAPGVGDCLRQQLGSRLAWTLTANQPSSQATTSKGVPSAVSRNMTRSRREGDTG
GTMEITEAALVRDILYVFQGIDGKNIKMNNTENCYKVEGKANLSRSLRDTAVRLSELGWL
HNKIRRYTDQRSLDRSFGLVGQSFCAALHQELREYYRLLSVLHSQLQLEDDQGVNLGLES
SLTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLVQHILS
LVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFMTMDQS
RKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPQDAADLFTDLENAFQGKIDAAYFE
TSKYLLDVLNKKYSLLDHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNLTGI
LETAVRATNAQFDSPEILRRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECMSHY
LRVFNFLWRAKRMEYILTDIRKGHMCNAKLLRNMPEFSGVLHQCHILASEMVHFIHQMQY
YITFEVLECSWDELWNKVQQAQDLDHIIAAHEVFLDTIISRCLLDSDSRALLNQLRAVFD
QIIELQNAQDAIYRAALEELQRRLQFEEKKKQREIEGQWGVTAAEEEEENKRIGEFKESI
PKMCSQLRILTHFYQGIVQQFLVLLTTSSDESLRFLSFRLDFNEHYKAREPRLRVSLGTR
GRRSSHT
Sequence of entity 4 (A, C, E, G), FASTA
>7QJA_4 Gamma-tubulin complex component 2 (chains A, C, E, G)
MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTP
EDFLKKYDELKSKNTRNLDPLVYLLSKLTEDKETLQYLQQNAKERAELAAAAVGSSTTSI
NVPAAASKISMQELEELRKQLGSVATGSTLQQSLELKRKMLRDKQNKKNSGQHLPIFPAW
VYERPALIGDFLIGAGISTDTALPIGTLPLASQESAVVEDLLYVLVGVDGRYVSAQPLAG
RQSRTFLVDPNLDLSIRELVHRILPVAASYSAVTRFIEEKSSFEYGQVNHALAAAMRTLV
KEHLILVSQLEQLHRQGLLSLQKLWFYIQPAMRTMDILASLATSVDKGECLGGSTLSLLH
DRSFSYTGDSQAQELCLYLTKAASAPYFEVLEKWIYRGIIHDPYSEFMVEEHELRKERIQ
EDYNDKYWDQRYTIVQQQIPSFLQKMADKILSTGKYLNVVRECGHDVTCPVAKEIIYTLK
ERAYVEQIEKAFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFMDLAEEELR
KPVEDITPPRLEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAM
AHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWI
SNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNID
DVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTL
EHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARL
SIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVT
AQ
Sequence of entity 5 (I, K), FASTA
>7QJA_5 Gamma-tubulin complex component 4 (chains I, K)
MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNRLCRLGTDYIRFTEFIE
QYTGHVQQQDHHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQALLDLEQEFLGDPHLSIS
HVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQILETVYKHSCGGLPPVRSALEKILAVCH
GVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGNVSAQPEEDEEDLGIGGLTGKQLRELQD
LRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKILFVGESVQMFENQNVNLTRKGSIL
KNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDF
YLLGRGELFQAFIDTAQHMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEY
HGKEHKADATQAREGPSRETSPREAPASGWAALGLSYKVQWPLHILFTPAVLEKYNVVFK
YLLSVRRVQAELQHCWALQMQRKHLKSNQTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQ
FSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQN
LGPLDERGAAQLSILVKGFSRQSSLLFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGG
TLGSFGM
Sequence of entity 6 (L, c), FASTA
>7QJA_6 Gamma-tubulin complex component 6 (chains L, c)
MASITQLFDDLCEALLPAAKTHLGQRSVNRKRAKRSLKKVAYNALFTNLFQDETQQLQPD
MSKLPARNKILMLSFDLRVGGLGPKADRLEELVEELEAAPCCPLLEVGSVLDLLVQLAGS
GPPQVLPRKRDYFLNNKHVGRNVPYSGYDCDDLSVFEMDVQSLISREECLCHSMIQETLQ
VMEAAPGTGLPTVGLFSFGDPCGDRFERDTRVSLFGALVHSRTYDMDVRLGLPPVPDNAD
LSGLAIKVPPSVDQWEDEGFQSASNLTPDSQSEPSVTPDVDLWEAALTYEASKRRCWERV
GCPPGHREEPYLTEAGRDAFDKFCRLHQGELQLLAGGVLQAPQPVLVKECELVKDVLNVL
IGVVSATFSLCQPAQAFVVKRGVHVSGASPESISSLLSEVAEYGTCYTRLSHFSLQPVLD
SLYSKGLVFQAFTSGLRRYLQYYRACVLSTPPTLSLLTIGFLFKKLGRQLRYLAELCGVG
AVLPGTCGGGPRAAFPTGVKLLSYLYQEALHNCSNEHYPVLLSLLKTSCEPYTRFIHDWV
YSGVFRDAYGEFMIQVNHEYLSFRDKLYWTHGYVLISKEVEDCVPVFLKHIAHDIYVCGK
TINLLKLCCPRHYLCWSDVPVPRISVIFSLEELKEIEKDCAVYVGRMERVARHSSVSKEE
KELRMEIAKQELIAHAREAASRVLSALSDRQMSERMALDARKREQFQRLKEQFVKDQERR
QAARQEELDDDFSYARELRDRERRLKSLEEELERKARQALVDHYSKLSAEAARREQKALW
RIQRHRLESARLRFLLEDEKHIQEMLKAVSEAHQPQEPPDVLLSVHPQVTSPGPEHPEGG
QGCDSGSAEQHSPAWDGWNRPGLLTPQPLKPLAVGAGGRGLQQAEGARPFSDSLSIGDFL
PVGPGAEPSVQTGMVPLLEVALQTINLDLPPSAPGEAPAAASTQPSRPQEYDFSTVLRPA
VATSPAPGPLQAAECSLGSSGLQLWEDSCGKMDACGSASRETLLPSHPPRRAALEEGSSQ
PTERLFGQVSGGGLPTGDYASEIAPTRPRWNTHGHVSDASIRVGENVSDVAPTQPRWNTH
GHVSNASISLGESVSDVAPTRPRWNIHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSNA
SIRVGENVSDVAPTRPRWNTHGHVSDASISLGESVSDMAPARPRWNTHGHVSDASISLGE
SVSDMAPTRPRWNTHGHVSDTSIRVGENVSDVAPIRSRCNTHGHVSDASISLGEPVSDVV
STRPRWNTHVPIPPPHMVLGALSPEAEPNTPRPQQSPPGHTSQSALSLGAQSTVLDCGPR
LPVEVGPSLSSPSSGCGEGSISVGENVSDVAPTQPWWPNTPGDSVSEELGPGRSGDTEDL
SPNWPLNSQEDTAAQSSPGRGEEAEASAAEAQGGEQAYLAGLAGQYHLERYPDSYESMSE
PPIAHLLRPVLPRAFAFPVDPQVQSAADETAVQLSELLTLPVLMKRSITAPLAAHISLVN
KAAVDYFFVELHLEAHYEALRHFLLMEDGEFAQSLSDLLFEKLGAGQTPGELLNPLVLNS
VLSKALQCSLHGDTPHASNLSLALKYLPEVFAPNAPDVLSCLELRYKVDWPLNIVITEGC
VSKYSGVFSFLLQLKLMMWALKDVCFHLKRTALLSHMAGSVQFRQLQLFKHEMQHFVKVI
QGYIANQILHVTWCEFRARLATVGDLEEIQRAHAEYLHKAVFRGLLTEKAAPVMNVIHSI
FSLVLKFRSQLISQAWGPPGGPRGAEHPNFALMQQSYNTFKYYSHFLFKVVTKLVNRGYQ
PHLEDFLLRINFNNYYQDA
Sequence of entity 7 (b, d, g, i, k, m), FASTA
>7QJA_7 Mitotic-spindle organizing protein 1 (chains b, d, g, i, k, m)
MASSSGAGAAAAAAAANLNAVRETMDVLLEISRILNTGLDMETLSICVRLCEQGINPEAL
SSVIKELRKATEALKAAENMTS
Sequence of entity 8 (O, P, Q, R, S, T, U, V, W, X, Y, Z), FASTA
>7QJA_8 Tubulin gamma-1 chain (chains O, P, Q, R, S, T, U, V, W, X, Y, Z)
MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHY
IPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFD
IIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSD
VVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSAST
TTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQP
KNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVAL
SRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDE
MDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Primary citation

Modular assembly of the principal microtubule nucleator gamma-TuRC. Wurtz, M., Zupa, E., Atorino, E.S. et al. Nat Commun (2022) 13:473-473. DOI 10.1038/s41467-022-28079-0 · PubMed

Other PDB entries of the same protein (UniProt A0A6I9HGD1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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