7RCU: Synthetic Max homodimer mimic
Synthetic Max homodimer mimic in complex with DNA. Determined by X-ray diffraction at 2.69 Å resolution. Released 14 Sept 2022.
- Method
- X-ray diffraction
- Resolution
- 2.69 Å
- Organisms
- Homo sapiens, synthetic construct
- Chains
- 24
- Atoms
- 5,840
- Mol. weight
- 90.42 kDa
- Ligands
- ACM, D5M, V7J
- Released
- 14 Sept 2022
Explore 7RCU in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7RCU contains 16 α-helices and 0 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains A, I and M: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-39 | 24 | |
Chains B and E: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-37 | 22 | |
Chain F: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-38 | 23 | |
Chain J: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-39 | 25 | |
Chain N: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-37 | 23 | |
Chains Q, R, V and W: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 51-64 | 14 | |
Chain S: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 50-65 | 16 | |
Chain T: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 50-64 | 15 | |
2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Protein max | A, B, E, F, I, J, M, N | protein | 28 | Homo sapiens | P61244 (AlphaFold model) |
| Protein max | Q, R, S, T, U, V, W, X | protein | 21 | Homo sapiens | P61244 (AlphaFold model) |
| DNA (5'-d(*a*gp*tp*ap*gp*cp*ap*cp*gp*tp*gp*cp*tp*ap*cp*tp*a)-3') | C, G, H, K, L, O, P | DNA | 17 | synthetic construct | |
| DNA (5'-d(*g*tp*ap*gp*cp*ap*cp*gp*tp*gp*cp*tp*ap*cp*tp*a)-3') | D | DNA | 16 | synthetic construct | |
Sequence of entity 1 (A, B, E, F, I, J, M, N), FASTA
>7RCU_1 Protein max (chains A, B, E, F, I, J, M, N)
KRAHHNALERKRRDHIKDSFHKLRDSVP
Sequence of entity 2 (Q, R, S, T, U, V, W, X), FASTA
>7RCU_2 Protein max (chains Q, R, S, T, U, V, W, X)
XSRAQILCKATEYIQYMRRKN
Sequence of entity 3 (C, G, H, K, L, O, P), FASTA
>7RCU_3 DNA (5'-D(*A*GP*TP*AP*GP*CP*AP*CP*GP*TP*GP*CP*TP*AP*CP*TP*A)-3') (chains C, G, H, K, L, O, P)
AGTAGCACGTGCTACTA
Sequence of entity 4 (D), FASTA
>7RCU_4 DNA (5'-D(*G*TP*AP*GP*CP*AP*CP*GP*TP*GP*CP*TP*AP*CP*TP*A)-3') (chains D)
GTAGCACGTGCTACTA
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ACM | Acetamide | C2 H5 N O | 1 |
| D5M | 2'-deoxyadenosine-5'-monophosphate | C10 H14 N5 O6 P | 1 |
| V7J | 2-(2,5-dioxopyrrolidin-1-yl)acetamide | C6 H8 N2 O3 | 6 |
Primary citation
Targeting MYC with modular synthetic transcriptional repressors derived from bHLH DNA-binding domains. Speltz, T.E., Qiao, Z., Swenson, C.S. et al. Nat Biotechnol (2023) 41:541-551. DOI 10.1038/s41587-022-01504-x · PubMed
Other PDB entries of the same protein (UniProt P61244 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6G6K 1.35 Å, The crystal structures of Human MYC:MAX bHLHZip complex
- 1NKP 1.8 Å, Crystal structure of Myc-Max recognizing DNA
- 1NLW 2.0 Å, Crystal structure of Mad-Max recognizing DNA
- 6G6L 2.2 Å, The crystal structures of Human MYC:MAX bHLHZip complex
- 6G6J 2.25 Å, The crystal structures of Human MYC:MAX bHLHZip complex
- 5EYO 2.39 Å, The crystal structure of the Max bHLH domain in complex with 5-carboxyl cytosine DNA
- 1HLO 2.8 Å, The crystal structure of an intact human max-DNA complex: new insights into mechanisms…
- 1AN2 2.9 Å, Recognition by max of its cognate DNA through a dimeric b/hlh/z domain
- 8OTS 3.3 Å, OCT4 and MYC-MAX co-bound to a nucleosome
- 8OTT 3.3 Å, MYC-MAX bound to a nucleosome at SHL+5.8
- 1R05 Solution Structure of Max B-HLH-LZ
Browse structure collections
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