7RYC: Oxytocin receptor

Oxytocin receptor (OTR) bound to oxytocin in complex with a heterotrimeric Gq protein. Determined by electron microscopy at 2.9 Å resolution. Released 9 Mar 2022.

Method: Electron microscopy
Resolution: 2.9 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,277
Mol. weight: 152.68 kDa
Ligands: MG
Released: 9 Mar 2022

Explore 7RYC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7RYC contains 32 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	6-23	18
α-helix	30-33	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	241-245	5	8
β-strand	250-252	3	8
β-strand	262-264	3	8
α-helix	272	1
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	319-320	2	3
β-strand	327-329	3	3
β-strand	336-339	4	3

Chain D: 14 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-23	12
α-helix	30-33	4
α-helix	35-43	9
α-helix	45-47	3
α-helix	53-55	3
α-helix	1007-1030	24
β-strand	1034-1038	5	2
α-helix	1046-1048	3
β-strand	1070-1076	7	2
β-strand	1079-1085	7	2
α-helix	1096-1098	3
β-strand	1105-1111	7	2
α-helix	1118-1129	12
β-strand	1138-1144	7	2
α-helix	1146-1155	10
α-helix	1160-1162	3
α-helix	1165-1168	4
α-helix	1184-1203	20
β-strand	1211-1215	5	2
α-helix	1224-1239	16

Chain E: 1 helix, 27 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	10
β-strand	10-12	3	11
β-strand	17-24	8	10
α-helix	29-31	3
β-strand	33-39	7	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	68-73	6	10
β-strand	78-84	7	10
β-strand	92-99	8	11
β-strand	111	1	11
β-strand	113	1	10
β-strand	115-119	5	11
β-strand	141	1	12
β-strand	146-148	3	13
β-strand	157	1	14
β-strand	160	1	12
β-strand	166	1	15
β-strand	172	1	15
β-strand	174-178	5	16
β-strand	179	1	13
β-strand	186-190	5	16
β-strand	194-195	2	16
β-strand	205-207	3	14
β-strand	212-214	3	14
β-strand	226-227	2	13
β-strand	229-231	3	16
β-strand	243-246	4	13

Chain L: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	5-8	4

Chain O: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	36-66	31
α-helix	75-91	17
α-helix	94-101	8
α-helix	109-142	34
α-helix	150-167	18
α-helix	171-174	4
β-strand	175-181	7	1
β-strand	184-189	6	1
α-helix	196-205	10
α-helix	206-210	5
α-helix	211-234	24
α-helix	274-298	25
α-helix	309-315	7
α-helix	321-331	11
α-helix	335-344	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Oxytocin	L	protein	10	Homo sapiens	P01178 (AlphaFold model)
Oxytocin receptor	O	protein	450	Homo sapiens	P30559 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Guanine nucleotide-binding…	D	protein	326	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	345	Homo sapiens	P62873 (AlphaFold model)
scFv16	E	protein	260	Mus musculus

Sequence of entity 1 (L), FASTA

>7RYC_1 Oxytocin (chains L)
CYIQNCPLGX

Sequence of entity 2 (O), FASTA

>7RYC_2 Oxytocin receptor (chains O)
MKTIIALSYIFCLVFADYKDDDDAMGQPGNGSAFLLAPNRSHAPDHDVENLYFQGMEGAL
AANWSAEAANASAAPPGAEGNRTAGPPRRNEALARVEVAVLCLILLLALSGNACVLLALR
TTRQKHSRLFFFMKHLSIADLVVAVFQVLPQLLWDITFRFYGPDLLCRLVKYLQVVGMFA
STYLLLLMSLDRCLAICQPLRSLRRRTDRLAVLATWLGCLVASAPQVHIFSLREVADGVF
DCWAVFIQPWGPKAYITWITLAVYIVPVIVLAACYGLISFKIWQNLRLKTAAAAAAEAPE
GAAAGDGGRVALARVSSVKLISKAKIRTVKMTFIIVLAFIVCWTPFFFVQMWSVWDANAP
KEASAFIIVMLLASLNSCCNPWIYMLFTGHLFHELVQRFLCCSASYLKGRRLGETSASKK
SNSSSFVLSHRSSSQRSCSQPSTALEVLFQ

Sequence of entity 3 (D), FASTA

>7RYC_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSAMGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLG
ADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCF
NDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKI
EDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVD
TENARRIFNDCKDIILQMNLREYNLV

Sequence of entity 4 (C), FASTA

>7RYC_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
GPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (E), FASTA

>7RYC_5 scFv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAASSEDLYFQ

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	1

Primary citation

The oxytocin signaling complex reveals a molecular switch for cation dependence. Meyerowitz, J.G., Robertson, M.J., Barros-Alvarez, X. et al. Nat Struct Mol Biol (2022) 29:274-281. DOI 10.1038/s41594-022-00728-4 · PubMed

Other PDB entries of the same protein (UniProt P01178 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7QVM 3.25 Å, Human Oxytocin receptor (OTR) oxytocin Gq chimera (mGoqi) complex
7OFG Oxytocin NMR solution structure
7OTD Oxytocin NMR solution structure

Browse structure collections

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