7S0F: Isoproterenol bound beta1 adrenergic receptor

Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric Gi protein. Determined by electron microscopy at 2.96 Å resolution. Released 17 Nov 2021.

Method: Electron microscopy
Resolution: 2.96 Å
Organisms: Meleagris gallopavo, Bos taurus, Rattus norvegicus
Chains: 4
Atoms: 6,998
Mol. weight: 146.45 kDa
Ligands: 5FW
Released: 17 Nov 2021

Explore 7S0F in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7S0F contains 28 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-39	6	8
α-helix	46-53	8
β-strand	185-191	7	8
β-strand	194-200	7	8
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	8
α-helix	242-254	13
β-strand	263-269	7	8
α-helix	271-280	10
α-helix	296-308	13
β-strand	319-323	5	8
α-helix	331-351	21

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	89-94	6	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-151	6	4
β-strand	156-161	6	4
β-strand	165-170	6	4
β-strand	175-181	7	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	249-254	6	6
β-strand	260-265	6	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	304-308	5	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain G: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	30-43	14
α-helix	45-47	3
α-helix	56-58	3
β-strand	59	1	9
β-strand	63	1	9

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	47-68	22
α-helix	76-89	14
α-helix	90-94	5
α-helix	95-104	10
α-helix	110-144	35
α-helix	146-152	7
α-helix	155-178	24
α-helix	190-194	5
α-helix	205-212	8
α-helix	213-217	5
α-helix	218-238	21
α-helix	284-315	32
α-helix	323-343	21
α-helix	347-355	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Beta1-Adrenergic Receptor	R	protein	508	Meleagris gallopavo	P07700 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	339	Bos taurus	P62871 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1,	A	protein	379	Rattus norvegicus	P10824 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>7S0F_1 Beta1-Adrenergic Receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDKLEVLFQGPNIFEMLRIDEGLRLKIYKDTEGYYTIGI
GHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYD
SLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRV
ITTFRTGTWDAYAAGAELLSQQWEAGMSLLMALVVLLIVAGNVLVIAAIGSTQRLQTLTN
LFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAI
DRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSFLPIMMHWWRDEDPQALKCYQDPG
CCDFVTNRAYAIASSIISFYIPLLIMIFVYLRVYREAKEQIRKIDRCEGRFREHKALKTL
GIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVFFNWLGYANSAFNPIIYCRSPDFRKA
FKRLLCFPRKADRRLEVLFQGPHHHHHH

Sequence of entity 2 (B), FASTA

>7S0F_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (A), FASTA

>7S0F_3 Guanine nucleotide-binding protein G(i) subunit alpha-1, (chains A)
MGSSHHHHHHSSGLEVLFQGPHMASMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVK
LLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDF
GDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYY
LNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCF
EGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFE
EKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFV
FDAVTDVIIKNNLKDCGLF

Sequence of entity 4 (G), FASTA

>7S0F_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFSAIL

Ligands and cofactors

ID	Name	Formula	Copies
5FW	Isoprenaline	C11 H17 N O3	1

Primary citation

Structural basis and mechanism of activation of two different families of G proteins by the same GPCR. Alegre, K.O., Paknejad, N., Su, M. et al. Nat Struct Mol Biol (2021) 28:936-944. DOI 10.1038/s41594-021-00679-2 · PubMed

Other PDB entries of the same protein (UniProt P07700 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4BVN 2.1 Å, Ultra-thermostable beta1-adrenoceptor with cyanopindolol bound
4AMJ 2.3 Å, Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist…
5A8E 2.4 Å, thermostabilised beta1-adrenoceptor with rationally designed inverse agonist…
2Y00 2.5 Å, Turkey BETA1 adrenergic receptor with stabilising mutations and bound partial agonist…
6H7N 2.5 Å, Activated turkey BETA1 adrenoceptor with bound partial agonist xamoterol and nanobody…
8DCS 2.5 Å, Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with…
2Y01 2.6 Å, Turkey BETA1 adrenergic receptor with stabilising mutations and bound partial agonist…
2Y02 2.6 Å, Turkey BETA1 adrenergic receptor with stabilising mutations and bound agonist carmoterol
7JJO 2.6 Å, Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound…
8DCR 2.6 Å, Cryo-EM structure of dobutamine-bound beta1-adrenergic receptor in complex with…
2VT4 2.7 Å, Turkey BETA1 adrenergic receptor with stabilising mutations and bound cyanopindolol
3ZPR 2.7 Å, Thermostabilised turkey beta1 adrenergic receptor with 4-methyl-2-(piperazin-1-yl)…

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