7TW8: PDB entry 7TW8

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to SAH. Determined by X-ray diffraction at 1.55 Å resolution. Released 7 Sept 2022.

Method: X-ray diffraction
Resolution: 1.55 Å
Organism: Severe acute respiratory syndrome coronavirus 2
Chains: 1
Atoms: 2,524
Mol. weight: 35.89 kDa
Ligands: ZN, SAH
Released: 7 Sept 2022

Explore 7TW8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TW8 contains 17 α-helices and 9 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	13-15	3
α-helix	18-32	15
α-helix	34-36	3
α-helix	46-49	4
α-helix	54-60	7
α-helix	65-77	13
α-helix	302-324	23
β-strand	328-332	5	1
β-strand	347-352	6	1
α-helix	360-362	3
β-strand	364-365	2	1
α-helix	370-373	4
β-strand	381-385	5	1
β-strand	396-401	6	1
α-helix	434-436	3
β-strand	439-441	3	1
β-strand	446-447	2	2
α-helix	451-452	2
α-helix	466-468	3
β-strand	473-474	2	2
α-helix	476-478	3
α-helix	485-503	19
β-strand	506-511	6	1
α-helix	517-520	4
α-helix	521-523	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Transcription factor ETV6,Proofreading exoribonuclease nsp14 chimera	A	protein	309	Severe acute respiratory syndrome coronavirus 2	P0DTD1 (AlphaFold model), P41212 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7TW8_1 Transcription factor ETV6,Proofreading exoribonuclease nsp14 chimera (chains A)
GSIALPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYR
SPHSGDELYELLQHILAQPAAGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAI
KCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIV
CRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQ
VVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYN
LWNTFTRLQ

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1
SAH	S-adenosyl-L-homocysteine	C14 H20 N6 O5 S	1

Primary citation

High-resolution structures of the SARS-CoV-2 N7-methyltransferase inform therapeutic development. Kottur, J., Rechkoblit, O., Quintana-Feliciano, R. et al. Nat Struct Mol Biol (2022) 29:850-853. DOI 10.1038/s41594-022-00828-1 · PubMed

Other PDB entries of the same protein (UniProt P0DTD1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

44PS 0.77 Å, 6-conformation model of SARS-CoV-2 NSP3 macrodomain (C2 crystal form, 100 K)
7KR0 0.77 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain (C2 crystal form, 100 K)
9CY0 0.8 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain in complex with AVI-4206
7TX0 0.84 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain in complex with ADP-ribose at pH 9 (P43…
7KQO 0.85 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain (P43 crystal form)
7HCO 0.87 Å, PanDDA analysis group deposition -- Crystal structure of SARS-CoV-2 NSP3 macrodomain in…
7KQP 0.88 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain in complex with ADP-ribose (P43 crystal…
7TWJ 0.9 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain at pH 4 (P43 crystal form)
7TWN 0.9 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain at pH 5 (P43 crystal form)
7TWO 0.9 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain at pH 6 (P43 crystal form)
7TWP 0.9 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain at pH 7 (P43 crystal form)
7TWQ 0.9 Å, Crystal structure of SARS-CoV-2 NSP3 macrodomain at pH 9 (P43 crystal form)

Browse structure collections

About this viewer

MolViewer shows 7TW8 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.