7TYH: Human Amylin2 Receptor

Human Amylin2 Receptor in complex with Gs and human calcitonin peptide. Determined by electron microscopy at 3.3 Å resolution. Released 23 Mar 2022.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 9,492
Mol. weight: 186.97 kDa
Released: 23 Mar 2022

Explore 7TYH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYH contains 47 α-helices and 64 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	14-38	25
β-strand	41-45	5	1
α-helix	47-48	2
α-helix	54-56	3
β-strand	207-213	7	1
β-strand	218-224	7	1
α-helix	234-238	5
β-strand	243-249	7	1
α-helix	265-277	13
β-strand	286-292	7	1
α-helix	294-300	7
α-helix	307-310	4
α-helix	332-351	20
β-strand	359-363	5	1
α-helix	371-390	20

Chain B: 4 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
β-strand	49-51	3	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78	1	3
β-strand	81-83	3	3
β-strand	88-91	4	3
α-helix	93	1
β-strand	94	1	3
α-helix	95	1
β-strand	103-105	3	4
β-strand	111-114	4	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-181	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	262-264	3	7
α-helix	272	1
β-strand	273-275	3	8
β-strand	278	1	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	306-308	3	8
β-strand	317-320	4	2
β-strand	327-330	4	2
β-strand	336-338	3	2

Chain E: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	61-76	16
α-helix	77-79	3
α-helix	90-103	14
α-helix	114-126	13
α-helix	148-155	8
α-helix	158-165	8

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-21	13
α-helix	30-33	4
α-helix	35-43	9
α-helix	53-55	3

Chain N: 0 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-5	3	9
β-strand	11-12	2	10
β-strand	17-19	3	11
β-strand	22	1	11
β-strand	23-25	3	9
β-strand	34-39	6	12
β-strand	45-51	7	12
β-strand	58-60	3	12
β-strand	65	1	11
β-strand	68-73	6	11
β-strand	78-84	7	11
β-strand	92-95	4	12
β-strand	98	1	12
β-strand	123-124	2	12
β-strand	125-126	2	10

Chain P: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8-21	14
α-helix	23-26	4

Chain R: 22 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	39-61	23
α-helix	63-64	2
β-strand	72	1	13
β-strand	75-76	2	14
β-strand	81-82	2	14
β-strand	85	1	13
β-strand	91	1	15
β-strand	94	1	16
α-helix	95-96	2
β-strand	107	1	16
β-strand	110	1	15
β-strand	112	1	17
β-strand	118	1	17
β-strand	120	1	18
β-strand	127	1	18
α-helix	132-135	4
α-helix	138-161	24
α-helix	164-170	7
α-helix	179-204	26
α-helix	209-213	5
α-helix	217-245	29
α-helix	262-267	6
α-helix	271-283	13
α-helix	296-298	3
α-helix	299-314	16
α-helix	319-328	10
α-helix	334-337	4
α-helix	339-343	5
α-helix	345-352	8
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-399	4
α-helix	401-404	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
nanobody 35	N	protein	138	Lama glama
Receptor activity-modifying protein 2	E	protein	156	Homo sapiens	O60895 (AlphaFold model)
Calcitonin	P	protein	33	Homo sapiens	P01258
Calcitonin receptor	R	protein	501	Homo sapiens	P30988

Sequence of entity 1 (A), FASTA

>7TYH_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7TYH_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7TYH_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>7TYH_4 nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (E), FASTA

>7TYH_5 Receptor activity-modifying protein 2 (chains E)
MKTIIALSYIFCLVFADYKDDDDKPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPI
EKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFS
DPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Sequence of entity 6 (P), FASTA

>7TYH_6 Calcitonin (chains P)
CGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPX

Sequence of entity 7 (R), FASTA

>7TYH_7 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Primary citation

A structural basis for amylin receptor phenotype. Cao, J., Belousoff, M.J., Liang, Y.L. et al. Science (2022) 375:eabm9609-eabm9609. DOI 10.1126/science.abm9609 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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