7TYL: Calcitonin Receptor

Calcitonin Receptor in complex with Gs and rat amylin peptide, bypass motif. Determined by electron microscopy at 3.3 Å resolution. Released 23 Mar 2022.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Lama glama, Rattus norvegicus
Chains: 6
Atoms: 8,978
Mol. weight: 169.63 kDa
Released: 23 Mar 2022

Explore 7TYL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYL contains 43 α-helices and 56 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-37	25
β-strand	40-47	8	1
α-helix	53-57	5
β-strand	207-213	7	1
β-strand	218-224	7	1
α-helix	235-238	4
β-strand	243-249	7	1
α-helix	267-277	11
β-strand	286-292	7	1
α-helix	294-303	10
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-352	21
β-strand	359-362	4	1
α-helix	371-390	20

Chain B: 3 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	91-94	4	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	157-161	5	5
β-strand	166-168	3	5
β-strand	178-180	3	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	217-223	7	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-263	4	7
α-helix	272-273	2
β-strand	276-277	2	8
β-strand	285-287	3	8
β-strand	294	1	9
β-strand	295-297	3	8
β-strand	308	1	9
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	10-23	14
α-helix	30-44	15
α-helix	45-47	3
α-helix	54-55	2

Chain N: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	10
β-strand	10-12	3	11
β-strand	18-25	8	10
α-helix	29-31	3
β-strand	34-39	6	12
β-strand	45-51	7	12
β-strand	58-60	3	12
α-helix	62-64	3
β-strand	69-73	5	10
β-strand	78-83	6	10
α-helix	88-90	3
β-strand	93-98	6	12
β-strand	118-122	5	12
β-strand	124-126	3	11

Chain P: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	5-8	4
α-helix	9-18	10
α-helix	27-31	5

Chain R: 21 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	42-60	19
β-strand	71-72	2	13
α-helix	73-74	2
β-strand	75-76	2	14
β-strand	81-82	2	14
β-strand	85-86	2	13
β-strand	92-94	3	15
β-strand	107-109	3	15
β-strand	110	1	16
β-strand	112-113	2	17
β-strand	117-118	2	17
β-strand	129	1	16
α-helix	132-135	4
α-helix	138-155	18
α-helix	157-159	3
α-helix	161-172	12
α-helix	179-203	25
α-helix	209-213	5
α-helix	217-248	32
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-280	10
α-helix	288-290	3
α-helix	296-298	3
α-helix	299-329	31
α-helix	334-352	19
α-helix	355-358	4
α-helix	366-381	16
α-helix	384-388	5
α-helix	389-393	5
α-helix	396-407	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	138	Lama glama
amylin peptide	P	protein	38	Rattus norvegicus	P12969 (AlphaFold model)
Calcitonin receptor	R	protein	501	Homo sapiens	P30988

Sequence of entity 1 (A), FASTA

>7TYL_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7TYL_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7TYL_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>7TYL_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (P), FASTA

>7TYL_5 amylin peptide (chains P)
KCNTATCATQRLANFLVRSSNNLGPVLPPTNVGSNTYX

Sequence of entity 6 (R), FASTA

>7TYL_6 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Primary citation

A structural basis for amylin receptor phenotype. Cao, J., Belousoff, M.J., Liang, Y.L. et al. Science (2022) 375:eabm9609-eabm9609. DOI 10.1126/science.abm9609 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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