7TYN: Calcitonin Receptor

Calcitonin Receptor in complex with Gs and salmon calcitonin peptide. Determined by electron microscopy at 2.6 Å resolution. Released 30 Mar 2022.

Method: Electron microscopy
Resolution: 2.6 Å
Organisms: Salmo salar, Homo sapiens, Lama glama
Chains: 6
Atoms: 9,364
Mol. weight: 174.15 kDa
Ligands: PLM, P42, NAG, Y01
Released: 30 Mar 2022

Explore 7TYN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYN contains 43 α-helices and 57 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-39	27
β-strand	41-47	7	6
α-helix	53-58	6
β-strand	208-214	7	6
β-strand	217-223	7	6
α-helix	234-238	5
β-strand	243-248	6	6
α-helix	265-277	13
β-strand	286-291	6	6
α-helix	294-300	7
α-helix	313-315	3
α-helix	332-352	21
β-strand	359-363	5	6
α-helix	371-390	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	7
β-strand	58-63	6	8
β-strand	69-74	6	8
β-strand	78-83	6	8
β-strand	89-94	6	8
β-strand	100-105	6	9
β-strand	111-116	6	9
β-strand	121-125	5	9
β-strand	134-139	6	9
β-strand	146-151	6	10
β-strand	156-161	6	10
β-strand	166-170	5	10
β-strand	175-180	6	10
β-strand	187-192	6	11
β-strand	198-203	6	11
β-strand	207-212	6	11
β-strand	218-223	6	11
β-strand	229-234	6	12
β-strand	240-245	6	12
β-strand	250-254	5	12
β-strand	259-264	6	12
α-helix	272	1
β-strand	273-278	6	13
β-strand	284-289	6	13
β-strand	294-298	5	13
β-strand	304-308	5	13
β-strand	315-320	6	7
β-strand	327-331	5	7
β-strand	336-339	4	7

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-23	14
α-helix	30-43	14
α-helix	53-55	3

Chain N: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	14
β-strand	10-12	3	15
β-strand	18-25	8	14
α-helix	29-31	3
β-strand	34-39	6	15
β-strand	45-51	7	15
β-strand	58-60	3	15
β-strand	68-73	6	14
β-strand	78-83	6	14
α-helix	88-90	3
β-strand	92-98	7	15
β-strand	118	1	15
β-strand	122-126	5	15

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	4	1	1
α-helix	5-7	3
α-helix	8-20	13
α-helix	23-25	3

Chain R: 23 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	39-61	23
α-helix	63-65	3
α-helix	71	1
β-strand	72	1	2
α-helix	73-74	2
β-strand	75-76	2	3
β-strand	81-82	2	3
β-strand	85	1	2
β-strand	89-94	6	4
α-helix	95-96	2
β-strand	107-112	6	4
β-strand	118	1	4
β-strand	120-121	2	5
β-strand	126-127	2	5
β-strand	130	1	4
α-helix	132-136	5
α-helix	138-172	35
α-helix	179-204	26
α-helix	209-214	6
α-helix	217-248	32
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-283	13
α-helix	288-290	3
α-helix	296-298	3
α-helix	299-328	30
α-helix	334-347	14
α-helix	349-352	4
α-helix	355-358	4
β-strand	361	1	1
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-408	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Calcitonin-1	P	protein	33	Salmo salar	B5XGR7 (AlphaFold model)
Calcitonin receptor	R	protein	501	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (P), FASTA

>7TYN_1 Calcitonin-1 (chains P)
CSNLSTCVLGKLSQELHKLQTYPRTNTGSGTPX

Sequence of entity 2 (R), FASTA

>7TYN_2 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Sequence of entity 3 (A), FASTA

>7TYN_3 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 4 (B), FASTA

>7TYN_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (G), FASTA

>7TYN_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (N), FASTA

>7TYN_6 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
PLM	Palmitic acid	C16 H32 O2	13
P42	(2S)-2-{[(1R)-1-hydroxyhexadecyl]oxy}-3-{[(1R)-1-hydroxyoctadecyl]oxy}propyl…	C42 H88 N O8 P	1
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2
Y01	Cholesterol hemisuccinate	C31 H50 O4	1

Primary citation

A structural basis for amylin receptor phenotype. Cao, J., Belousoff, M.J., Liang, Y.L. et al. Science (2022) 375:eabm9609-eabm9609. DOI 10.1126/science.abm9609 · PubMed

Other PDB entries of the same protein (UniProt B5XGR7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7TYW 3.0 Å, Human Amylin1 Receptor in complex with Gs and salmon calcitonin peptide
7TYY 3.0 Å, Human Amylin2 Receptor in complex with Gs and salmon calcitonin peptide

Browse structure collections

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