7TYW: Human Amylin1 Receptor

Human Amylin1 Receptor in complex with Gs and salmon calcitonin peptide. Determined by electron microscopy at 3.0 Å resolution. Released 23 Mar 2022.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Salmo salar, Lama glama
Chains: 7
Atoms: 9,779
Mol. weight: 188.14 kDa
Ligands: PLM, Y01, NAG
Released: 23 Mar 2022

Explore 7TYW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYW contains 49 α-helices and 56 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-39	27
β-strand	41-45	5	6
β-strand	207-214	8	6
β-strand	217-224	8	6
α-helix	234-238	5
β-strand	243-249	7	6
α-helix	265-277	13
β-strand	286-292	7	6
α-helix	294-303	10
α-helix	308-311	4
α-helix	313-315	3
α-helix	332-352	21
β-strand	359-363	5	6
α-helix	371-390	20

Chain B: 4 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
β-strand	43	1	7
β-strand	48-51	4	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	89-94	6	9
α-helix	95	1
β-strand	103-105	3	10
β-strand	111-114	4	10
β-strand	121-125	5	10
β-strand	134-139	6	10
β-strand	148-151	4	11
β-strand	156-160	5	11
β-strand	166-170	5	11
β-strand	176-180	5	11
β-strand	189-192	4	12
β-strand	198-202	5	12
β-strand	208-212	5	12
β-strand	221-222	2	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	259-264	6	13
α-helix	272	1
β-strand	273-278	6	14
β-strand	284-289	6	14
β-strand	294-298	5	14
β-strand	304-307	4	14
β-strand	318-320	3	8
β-strand	327-329	3	8
β-strand	336-338	3	8

Chain E: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	30-34	5
α-helix	35-37	3
α-helix	41-51	11
α-helix	64-67	4
α-helix	70-79	10
α-helix	87-97	11
α-helix	120-124	5
α-helix	125-142	18

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-21	13
α-helix	27-29	3
α-helix	30-42	13
α-helix	53-55	3

Chain N: 2 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	15
β-strand	10-11	2	16
β-strand	22-25	4	15
α-helix	29-31	3
β-strand	34-39	6	16
β-strand	45-51	7	16
β-strand	58-60	3	16
β-strand	71-73	3	15
β-strand	78-80	3	15
α-helix	88-90	3
β-strand	92-98	7	16
β-strand	122-125	4	16

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	4	1	1
α-helix	5-7	3
α-helix	8-21	14
α-helix	23-25	3

Chain R: 20 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	40-61	22
β-strand	72	1	2
β-strand	76	1	3
β-strand	81	1	3
β-strand	85	1	2
β-strand	92-94	3	4
α-helix	95-96	2
β-strand	107-109	3	4
β-strand	120	1	5
β-strand	127	1	5
β-strand	130	1	4
α-helix	138-172	35
α-helix	174-176	3
α-helix	179-204	26
α-helix	209-214	6
α-helix	218-245	28
α-helix	246-250	5
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-283	13
α-helix	296-298	3
α-helix	299-327	29
α-helix	334-346	13
α-helix	349-352	4
α-helix	355-358	4
β-strand	361	1	1
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-403	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Receptor activity-modifying protein 1	E	protein	149	Homo sapiens	O60894 (AlphaFold model)
Calcitonin-1	P	protein	33	Salmo salar	B5XGR7 (AlphaFold model)
Calcitonin receptor	R	protein	501	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (E), FASTA

>7TYW_1 Receptor activity-modifying protein 1 (chains E)
MKTIIALSYIFCLVFADYKDDDDKHGSCQEANYGALLRELCLTQFQVDMEAVGETLWCDW
GRTIRSYRELADCTWHMAEKLGCFWPNAEVDRFFLAVHGRYFRSCPISGRAVRDPPGSIL
YPFIVVPITVTLLVTALVVWQSKRTEGIV

Sequence of entity 2 (P), FASTA

>7TYW_2 Calcitonin-1 (chains P)
CSNLSTCVLGKLSQELHKLQTYPRTNTGSGTPX

Sequence of entity 3 (R), FASTA

>7TYW_3 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Sequence of entity 4 (A), FASTA

>7TYW_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 5 (B), FASTA

>7TYW_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 6 (G), FASTA

>7TYW_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 7 (N), FASTA

>7TYW_7 nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
PLM	Palmitic acid	C16 H32 O2	2
Y01	Cholesterol hemisuccinate	C31 H50 O4	2
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2

Primary citation

A structural basis for amylin receptor phenotype. Cao, J., Belousoff, M.J., Liang, Y.L. et al. Science (2022) 375:eabm9609-eabm9609. DOI 10.1126/science.abm9609 · PubMed

Other PDB entries of the same protein (UniProt O60894 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6ZHO 1.6 Å, Crystal structure of a CGRP receptor ectodomain heterodimer with bound high affinity…
8AX7 1.65 Å, Crystal structure of a CGRP receptor ectodomain heterodimer bound to macrocyclic…
7P0F 1.85 Å, Crystal structure of a CGRP receptor ectodomain heterodimer bound to macrocyclic…
8AX6 1.9 Å, Crystal structure of a CGRP receptor ectodomain heterodimer bound to macrocyclic…
6D1U 2.05 Å, Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer in complex…
3N7S 2.1 Å, Crystal structure of the ectodomain complex of the CGRP receptor, a Class-B GPCR,…
7TYF 2.2 Å, Human Amylin1 Receptor in complex with Gs and rat amylin peptide
9BP3 2.2 Å, Human Amylin1 Receptor in complex with Gs and cagrilintide
7P0I 2.3 Å, Crystal structure of a CGRP receptor ectodomain heterodimer bound to macrocyclic…
2YX8 2.4 Å, Crystal structure of the extracellular domain of human RAMP1
9AUC 2.4 Å, Human Amylin1 Receptor in Complex with Gs and human Calcitonin Gene-Related Peptide
4RWG 2.44 Å, Crystal structure of the CLR:RAMP1 extracellular domain heterodimer with bound high…

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