7UIG: Mediator-PIC Early
Mediator-PIC Early (Mediator A). Determined by electron microscopy at 4.3 Å resolution. Released 15 Feb 2023.
- Method
- Electron microscopy
- Resolution
- 4.3 Å
- Organism
- Saccharomyces cerevisiae
- Chains
- 17
- Atoms
- 27,580
- Mol. weight
- 749.71 kDa
- Released
- 15 Feb 2023
Explore 7UIG in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7UIG contains 136 α-helices and 96 β-strands across 17 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain a: 12 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-21 | 15 | |
| α-helix | 28-37 | 10 | |
| β-strand | 41-46 | 6 | 1 |
| β-strand | 51-57 | 7 | 1 |
| β-strand | 61-68 | 8 | 1 |
| β-strand | 73-81 | 9 | 1 |
| β-strand | 91 | 1 | 2 |
| β-strand | 102 | 1 | 2 |
| α-helix | 103-109 | 7 | |
| α-helix | 115-130 | 16 | |
| α-helix | 164-181 | 18 | |
| β-strand | 187-190 | 4 | 3 |
| β-strand | 198-203 | 6 | 3 |
| β-strand | 209-219 | 11 | 3 |
| β-strand | 227-229 | 3 | 4 |
| β-strand | 232-234 | 3 | 5 |
| β-strand | 239-241 | 3 | 5 |
| β-strand | 248-250 | 3 | 4 |
| β-strand | 252-258 | 7 | 3 |
| β-strand | 272-274 | 3 | 6 |
| α-helix | 280-282 | 3 | |
| β-strand | 283-286 | 4 | 3 |
| α-helix | 298-304 | 7 | |
| β-strand | 312-316 | 5 | 7 |
| β-strand | 322-324 | 3 | 6 |
| β-strand | 325-329 | 5 | 3 |
| α-helix | 332-336 | 5 | |
| α-helix | 337-347 | 11 | |
| α-helix | 348-352 | 5 | |
| α-helix | 353-360 | 8 | |
| β-strand | 528-532 | 5 | 7 |
| β-strand | 536-539 | 4 | 7 |
| β-strand | 543-546 | 4 | 7 |
| α-helix | 551-565 | 15 | |
Chain d: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-39 | 8 | |
| α-helix | 41-61 | 21 | |
| α-helix | 67-88 | 22 | |
| α-helix | 92-127 | 36 | |
| α-helix | 132-147 | 16 | |
| α-helix | 150-164 | 15 | |
| α-helix | 185-190 | 6 | |
| α-helix | 192-198 | 7 | |
Chain f: 6 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-19 | 6 | |
| α-helix | 27-32 | 6 | |
| α-helix | 42-55 | 14 | |
| α-helix | 89-96 | 8 | |
| α-helix | 98-107 | 10 | |
| β-strand | 113-121 | 9 | 8 |
| β-strand | 125-134 | 10 | 8 |
| β-strand | 146-156 | 11 | 8 |
| β-strand | 159-161 | 3 | 8 |
| α-helix | 166-191 | 26 | |
| β-strand | 193-195 | 3 | 9 |
| β-strand | 199-201 | 3 | 9 |
Chain g: 8 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-17 | 4 | |
| α-helix | 19-22 | 4 | |
| α-helix | 25-42 | 18 | |
| α-helix | 67-71 | 5 | |
| β-strand | 76-78 | 3 | 10 |
| β-strand | 81-83 | 3 | 10 |
| α-helix | 112-139 | 28 | |
| α-helix | 141-143 | 3 | |
| α-helix | 145-165 | 21 | |
| α-helix | 170-212 | 43 | |
Chain h: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 31-54 | 24 | |
| α-helix | 62-85 | 24 | |
| α-helix | 87-92 | 6 | |
| α-helix | 103-114 | 12 | |
| α-helix | 117-119 | 3 | |
| α-helix | 120-133 | 14 | |
| α-helix | 144-169 | 26 | |
Chain i: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 66-78 | 13 | |
| α-helix | 84-107 | 24 | |
| α-helix | 109-114 | 6 | |
| α-helix | 120-147 | 28 | |
Chain j: 6 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-37 | 36 | |
| α-helix | 43-67 | 25 | |
| α-helix | 81-86 | 6 | |
| β-strand | 93 | 1 | 11 |
| α-helix | 95-132 | 38 | |
| α-helix | 134-136 | 3 | |
| α-helix | 137-147 | 11 | |
Chain k: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-39 | 36 | |
| α-helix | 45-76 | 32 | |
| α-helix | 90-109 | 20 | |
| α-helix | 111-113 | 3 | |
9 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Mediator of RNA polymerase II transcription subunit 1 | a | protein | 566 | Saccharomyces cerevisiae | Q12321 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 4 | d | protein | 284 | Saccharomyces cerevisiae | Q12343 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 6 | f | protein | 295 | Saccharomyces cerevisiae | P38782 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 7 | g | protein | 222 | Saccharomyces cerevisiae | Q08278 (AlphaFold model) |
| Mediator of RNA polymerase II transcription subunit 8 | h | protein | 223 | Saccharomyces cerevisiae | P38304 |
| Mediator of RNA polymerase II transcription subunit 9 | i | protein | 149 | Saccharomyces cerevisiae | P33308 |
| Mediator of RNA polymerase II transcription subunit 10 | j | protein | 157 | Saccharomyces cerevisiae | Q06213 |
| Mediator of RNA polymerase II transcription subunit 11 | k | protein | 115 | Saccharomyces cerevisiae | Q99278 |
| Mediator of RNA polymerase II transcription subunit 14 | n | protein | 1082 | Saccharomyces cerevisiae | P19263 |
| Mediator of RNA polymerase II transcription subunit 17 | q | protein | 687 | Saccharomyces cerevisiae | P32569 |
| Mediator of RNA polymerase II transcription subunit 18 | r | protein | 307 | Saccharomyces cerevisiae | P32585 |
| Mediator of RNA polymerase II transcription subunit 19 | s | protein | 220 | Saccharomyces cerevisiae | P25046 |
5 more molecules are not listed.
Sequence of entity 1 (a), FASTA
>7UIG_1 Mediator of RNA polymerase II transcription subunit 1 (chains a)
MVEGDSYVETLDSMIELFKDYKPGSITLENITRLCQTLGLESFTEELSNELSRLSTASKI
IVIDVDYNKKQDRIQDVKLVLASNFDNFDYFNQRDGEHEKSNILLNSLTKYPDLKAFHNN
LKFLYLLDAYSHIESDSTSHNNGSSDKSLDSSNASFNNQGKLDLFKYFTELSHYIRQCFQ
DNCCDFKVRTNLNDKFGIYILTQGINGKEVPLAKIYLEENKSDSQYRFYEYIYSQETKSW
INESAENFSNGISLVMEIVANAKESNYTDLIWFPEDFISPELIIDKVTCSSNSSSSPPII
DLFSNNNYNSRIQLMNDFTTKLINIKKFDISNDNLDLISEILKWVQWSRIVLQNVFKLVS
TPSSNSNSSELEPDYQAPFSTSTKDKNSSTSNTEPIPRSNRHGSVVEASRRRRSSTNKSK
RPSITEAMMLKEEGLQQFNLHEILSEPAIEEENGDSIKEHSTTMDGANDLGFTASVSNQE
NAGTDIVMEDHGVLQGTSQNYGTATADDADIEMKDVSSKPSKPESSVLQLIVSEDHIILD
TISECNLYDDVKCWSKFIEKFQDIVS
Sequence of entity 2 (d), FASTA
>7UIG_2 Mediator of RNA polymerase II transcription subunit 4 (chains d)
MSVQDTKAVEFSMGHIRSSSVSLVAEATSNTNSEDKLSKVQLYEDLCRYEDTLSKLVESV
DRFKPNLDIAKDLIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNE
CHDELKALPMLEQVEFEKNTILQQRSKINSTELLDYATKLSKFTKIPPTFDKGAVGPNNF
IWPAEDALRRGMLAMASLHSKELTRIPGEEVEETEVPTVPPSQSEEQKGQMAKKEGTPKT
DSFIFDGTAKEVGDEADNTKDKEKEENNDDALDLDLDLFDPDDF
Sequence of entity 3 (f), FASTA
>7UIG_3 Mediator of RNA polymerase II transcription subunit 6 (chains f)
MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA
AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV
REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH
LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT
VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI
Sequence of entity 4 (g), FASTA
>7UIG_4 Mediator of RNA polymerase II transcription subunit 7 (chains g)
MSNDPGNEVSSLYPPPPPYVKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITC
ALDYLIPPPMPKNQQYRAFGSIWQVKDQLPDLESMGLTQLYKKSTENESTNYQYKIQELR
KLLKSLLLNYLELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEE
QLEYKRGEIREIEQVCKQVHDKLTSIQDTLRTGSQSPPSSSQ
Sequence of entity 5 (h), FASTA
>7UIG_5 Mediator of RNA polymerase II transcription subunit 8 (chains h)
MSQSTASLVPEGNQGSLQEDVSFDFNGVPGQALDAVRMRLAQLTHSLRRIRDEMSKAELP
QWYTLQSQLNVTLSQLVSVTSTLQHFQETLDSTVVYPLPKFPTTSHESLVTTLLRKKNIP
EVDEWMKYVRETSGVTTALLKDEEIEKLLQQDREITNWARTTFRNEYGKHDFKNEESLSE
EHASLLVRDSKPSKPFNVDDVLKFTFTGEKPIITGSTSTSSSN
Sequence of entity 6 (i), FASTA
>7UIG_6 Mediator of RNA polymerase II transcription subunit 9 (chains i)
MNLQNNVLNQIHQILLPTNPTLDKPNAEATKEEFSSAENRDEKDYLTNQQPKNLSTPSTS
SNGEFIPHIFYSLHQIRKDPNNLSNQLETLTGSIRHRLKLCKSLISENEDTKDLLSKSPS
EWQDIIHQREQELQIKRDVLDDLYRKLQR
Sequence of entity 7 (j), FASTA
>7UIG_7 Mediator of RNA polymerase II transcription subunit 10 (chains j)
MNGNSTNNEQLQQELATTQDQVASIIESFVELGVSIYDFPGTPEATKGMITNLQRNVDRL
YKLNVRSNDPQSSLSKVDIPLEVVQYIEDGRNPDIYTREFVEAIRRSNQYQRGKMHGLKQ
LRDSLADKIVDEFPELKEPVEDIIKRTSPIDNVSNTH
Sequence of entity 8 (k), FASTA
>7UIG_8 Mediator of RNA polymerase II transcription subunit 11 (chains k)
MQPPYIQERLKSLNDIETQLCSMLQEASQVTFIFGELKRGNESVKPQFENHVKQFYERLD
KSTTQLRKEIQLLDENVGTRLLPINVNKKALGQDTEKMEEQLDLLSAILDPSKSK
Sequence of entity 9 (n), FASTA
>7UIG_9 Mediator of RNA polymerase II transcription subunit 14 (chains n)
MTTTIGSPQMLANEERLSNEMHALKNRSEQNGQEQQGPVKNTQLHGPSATDPETTATQKE
SLEMVPKDTSAATMTSAPPPALPHVEINQVSLALVIRNLTVFTMKELAQYMKTNVHTQAN
EPNSAKKIRFLQLIIFLRTQFLKLYVLVKWTRTIKQNNFHVLIDLLNWFRTTNMNVNNCI
WALKSSLNSMTNAKLPNVDLVTALEVLSLGRPNLPTHNFKLSGVSNSMDMVDGMAKVPIG
LILQRLKDLNLTVSIKIALMNIPKPLNSYHIKNGRIYFTVPNEFEIQLSTVNRQSPLFFV
DLKLLFNTEAEQTVSAVTEATSTNGDSENNEENSSSNGNNLPLNKPRLEKLINEILLKSN
DPLLSLYNFLHKYVLTLQLYMVHREFLKLANGGKFSKSNLIHNYDSKKSTITVRYWLNGK
MDSKGKITIGIQRTTESLILKWDNQSASRAKNMPVIYNNIVSNIEGILDEIMFNHARIIR
SELLARDIFQEDEENSDVLLFQLPTTCVSMAPIQLKIDLLSGQFYFRNPTPLLSNYASKI
NRAEGPEELARILQQLKLDKIIHVLTTMFENTGWSCSRIIKIDKPIRTQVNTGGESVVKK
EDNKYAIAGNSTTNSDVSLLLQRDLFIRLPHWPLNWYLILSIISSKTSCVVEKRIGKIVS
QRGKWNLKYLDNSNVMTVKLESITYQKIMILQRTILNRIINHMLIDSLNQLEIRNKICSS
EMINEQKLPQYIIQGSNTNDNISIITLELESFLEGSKALNSILESSMFLRIDYSNSQIRL
YAKFKRNTMMIQCQIDKLYIHFVQEEPLAFYLEESFTNLGIIVQYLTKFRQKLMQLVVLT
DVVERLHKNFESENFKIIALQPNEISFKYLSNNDEDDKDCTIKISTNDDSIKNLTVQLSP
SNPQHIIQPFLDNSKMDYHFIFSYLQFTSSLFKALKVILNERGGKFHESGSQYSTMVNIG
LHNLNEYQIVYYNPQAGTKITICIELKTVLHNGRDKIQFHIHFADVAHITTKSPAYPMMH
QVRNQVFMLDTKRLGTPESVKPANASHAIRLGNGVACDPSEIEPILMEIHNILKVDSNSS
SS
Sequence of entity 10 (q), FASTA
>7UIG_10 Mediator of RNA polymerase II transcription subunit 17 (chains q)
MTTEDPDSNHLSSETGIKLALDPNLITLALSSNPNSSLHSPTSDEPVPESAGKADTSIRL
EGDELENKTKKDNDKNLKFLKNKDSLVSNPHEIYGSMPLEQLIPIILRQRGPGFKFVDLN
EKELQNEIKQLGSDSSDGHNSEKKDTDGADENVQIGEDFMEVDYEDKDNPVDSRNETDHK
TNENGETDDNIETVMTQEQFVKRRRDMLEHINLAMNESSLALEFVSLLLSSVKESTGMSS
MSPFLRKVVKPSSLNSDKIPYVAPTKKEYIELDILNKGWKLQSLNESKDLLRASFNKLSS
ILQNEHDYWNKIMQSISNKDVIFKIRDRTSGQKLLAIKYGYEDSGSTYKHDRGIANIRNN
IESQNLDLIPHSSSVFKGTDFVHSVKKFLRVRIFTKIESEDDYILSGESVMDRDSESEEA
ETKDIRKQIQLLKKIIFEKELMYQIKKECALLISYGVSIENENKVIIELPNEKFEIELLS
LDDDSIVNHEQDLPKINDKRANLMLVMLRLLLVVIFKKTLRSRISSPHGLINLNVDDDIL
IIRPILGKVRFANYKLLLKKIIKDYVLDIVPGSSITETEVEREQPQENKNIDDENITKLN
KEIRAFDKLLNIPRRELKINLPLTEHKSPNLSLMLESPNYCNALIHIKFSAGTEANAVSF
DTTFSDFKEVEDFLHFIVAEYIQQKKV
Sequence of entity 11 (r), FASTA
>7UIG_11 Mediator of RNA polymerase II transcription subunit 18 (chains r)
MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPNPSYDVENVNSRNQLVE
PNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNYAMTRNILHNTVPQVTNFN
STNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNESCSPWSLQISDIPAAGNNRSV
SMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTIGVKFFMKHGLILELQKIWQIEE
AGNSQITSGGFLLKAYINVSRGTDIDRINYTETALMNLKKELQGYIELSVPDRQSMDSRV
AHGNILI
Sequence of entity 12 (s), FASTA
>7UIG_12 Mediator of RNA polymerase II transcription subunit 19 (chains s)
MASRVDETTVPSYYYYVDPETTYTYQQPNPLQDLISVYGLDDISRQVARTNLDGTKAVKL
RKSYKNQIADLSGKFSTIPTRENGKGGQIAHILFQNNPDMMIQPPQQGQNMSEQQWREQL
RNRDIALFQPPNFDWDLCSSVLSQFERSYPSEFANQNQGGAQAPFDIDDLAFDLDGTGKS
QSGSNSGNNSKKRKNKSSGSSMATPTHSDSHEDMKRRRLE
Primary citation
Structural basis of a transcription pre-initiation complex on a divergent promoter. Gorbea Colon, J.J., Palao 3rd, L., Chen, S.F. et al. Mol Cell (2023) 83:574. DOI 10.1016/j.molcel.2023.01.011 · PubMed
Other PDB entries of the same protein (UniProt Q12321 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8CEN 3.0 Å, Yeast RNA polymerase II transcription pre-initiation complex with core Mediator
- 7UI9 3.3 Å, Core Mediator-PICearly (Copy A)
- 7UIO 3.3 Å, Mediator-PIC Early (Composite Model)
- 9PC8 3.4 Å, Core Mediator of PIC-Med-SWI/SNF
- 8CEO 3.6 Å, Yeast RNA polymerase II transcription pre-initiation complex with core Mediator and the…
- 7UIL 4.3 Å, Mediator-PIC Early (Tail A/B Dimer)
- 7UIF 4.6 Å, Mediator-PIC Early (Core B)
- 8ZZ3 Molecular architecture of the yeast Mediator complex
Browse structure collections
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