7UNK: Importin-4

Structure of Importin-4 bound to the H3-H4-ASF1 histone-histone chaperone complex. Determined by electron microscopy at 3.45 Å resolution. Released 21 Sept 2022.

Method
Electron microscopy
Resolution
3.45 Å
Organisms
Homo sapiens, Xenopus laevis, Saccharomyces cerevisiae
Chains
4
Atoms
10,992
Mol. weight
177.26 kDa
Released
21 Sept 2022

Explore 7UNK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7UNK contains 89 α-helices and 18 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 75 helices, 3 β-strands

ElementResiduesLengthSheet
α-helix21-255
α-helix41-444
α-helix53-6816
α-helix70-723
α-helix77-8812
α-helix95-11016
α-helix119-1268
α-helix132-14817
α-helix150-1534
α-helix157-16711
α-helix174-18714
α-helix188-1903
α-helix193-1953
α-helix196-21621
α-helix218-23215
α-helix240-2423
α-helix243-25412
α-helix261-27717
α-helix280-2845
α-helix288-29912
α-helix301-3044
α-helix310-3123
α-helix329-34214
α-helix346-36116
α-helix366-38217
α-helix384-3852
α-helix386-3916
α-helix394-3974
α-helix402-4043
α-helix408-42417
α-helix426-4294
α-helix430-4323
α-helix433-44614
α-helix4481
α-helix452-46615
α-helix470-4734
α-helix477-48913
α-helix495-51016
α-helix519-53113
α-helix535-5373
α-helix538-55417
α-helix557-5593
α-helix560-57415
α-helix580-5834
α-helix587-59711
α-helix599-6013
α-helix602-6098
α-helix613-6164
β-strand622-62651
α-helix629-6313
β-strand64312
α-helix645-6473
β-strand660-66561
α-helix670-68617
α-helix692-70413
α-helix705-7073
α-helix711-73424
α-helix738-75720
α-helix765-78117
α-helix783-7853
α-helix792-80413
α-helix812-8154
α-helix828-84619
α-helix852-86514
α-helix872-88918
α-helix890-8934
α-helix897-90711
α-helix913-92917
α-helix932-9376
α-helix938-95114
α-helix955-97016
α-helix981-9877
α-helix995-9973
α-helix998-10047
α-helix1020-103213
α-helix1038-105013
α-helix1057-10659
α-helix1068-10769
Chain C: 6 helices, 3 β-strands
ElementResiduesLengthSheet
α-helix2-43
β-strand4111
α-helix42-443
α-helix45-5814
α-helix64-7815
β-strand83-8423
α-helix86-11328
β-strand118-11922
α-helix121-13010
Chain D: 5 helices, 9 β-strands
ElementResiduesLengthSheet
β-strand6-1054
β-strand16-1725
α-helix21-222
β-strand25-2844
β-strand38-4475
β-strand55-6285
α-helix77-804
α-helix86-894
β-strand92-101105
β-strand104-117145
α-helix120-1245
α-helix132-1343
β-strand135-13955
β-strand145-14845
Chain E: 3 helices, 3 β-strands
ElementResiduesLengthSheet
α-helix31-4010
β-strand45-4622
α-helix50-7526
β-strand80-8123
α-helix83-908
β-strand95-9845

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Importin-4Aprotein1081Homo sapiensQ8TEX9 (AlphaFold model)
Histone H3Cprotein136Xenopus laevisQ92133 (AlphaFold model)
Histone chaperoneDprotein279Saccharomyces cerevisiaeP32447 (AlphaFold model)
Histone H4Eprotein103Xenopus laevisP62799 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7UNK_1 Importin-4 (chains A)
MESAGLEQLLRELLLPDTERIRRATEQLQIVLRAPAALPALCDLLASAADPQIRQFAAVL
TRRRLNTRWRRLAAEQRESLKSLILTALQRETEHCVSLSLAQLSATIFRKEGLEAWPQLL
QLLQHSTHSPHSPEREMGLLLLSVVVTSRPEAFQPHHRELLRLLNETLGEVGSPGLLFYS
LRTLTTMAPYLSTEDVPLARMLVPKLIMAMQTLIPIDEAKACEALEALDELLESEVPVIT
PYLSEVLTFCLEVARNVALGNAIRIRILCCLTFLVKVKSKALLKNRLLPPLLHTLFPIVA
AEPPPGQLDPEDQDSEEEELEIELMGETPKHFAVQVVDMLALHLPPEKLCPQLMPMLEEA
LRSESPYQRKAGLLVLAVLSDGAGDHIRQRLLPPLLQIVCKGLEDPSQVVRNAALFALGQ
FSENLQPHISSYSREVMPLLLAYLKSVPLGHTHHLAKACYALENFVENLGPKVQPYLPEL
MECMLQLLRNPSSPRAKELAVSALGAIATAAQASLLPYFPAIMEHLREFLLTGREDLQPV
QIQSLETLGVLARAVGEPMRPLAEECCQLGLGLCDQVDDPDLRRCTYSLFAALSGLMGEG
LAPHLEQITTLMLLSLRSTEGIVPQYDGSSSFLLFDDESDGEEEEELMDEDVEEEDDSEI
SGYSVENAFFDEKEDTCAAVGEISVNTSVAFLPYMESVFEEVFKLLECPHLNVRKAAHEA
LGQFCCALHKACQSCPSEPNTAALQAALARVVPSYMQAVNRERERQVVMAVLEALTGVLR
SCGTLTLKPPGRLAELCGVLKAVLQRKTACQDTDEEEEEEDDDQAEYDAMLLEHAGEAIP
ALAAAAGGDSFAPFFAGFLPLLVCKTKQGCTVAEKSFAVGTLAETIQGLGAASAQFVSRL
LPVLLSTAQEADPEVRSNAIFGMGVLAEHGGHPAQEHFPKLLGLLFPLLARERHDRVRDN
ICGALARLLMASPTRKPEPQVLAALLHALPLKEDLEEWVTIGRLFSFLYQSSPDQVIDVA
PELLRICSLILADNKIPPDTKAALLLLLTFLAKQHTDSFQAALGSLPVDKAQELQAVLGL
S
Sequence of entity 2 (C), FASTA
>7UNK_2 Histone H3 (chains C)
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCGIHAKRVTI
MPKDIQLARRIRGERA
Sequence of entity 3 (D), FASTA
>7UNK_3 Histone chaperone (chains D)
MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI
LVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE
ELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE
DDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE
EEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN
Sequence of entity 4 (E), FASTA
>7UNK_4 Histone H4 (chains E)
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Primary citation

Structure of IMPORTIN-4 bound to the H3-H4-ASF1 histone-histone chaperone complex. Bernardes, N.E., Fung, H.Y.J., Li, Y. et al. Proc Natl Acad Sci U S A (2022) 119:e2207177119-e2207177119. DOI 10.1073/pnas.2207177119 · PubMed

Other PDB entries of the same protein (UniProt Q8TEX9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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