8DYO: Importin-4

Cryo-EM structure of Importin-4 bound to RanGTP. Determined by electron microscopy at 7.1 Å resolution. Released 21 Sept 2022.

Method
Electron microscopy
Resolution
7.1 Å
Organisms
Homo sapiens, Saccharomyces cerevisiae
Chains
2
Atoms
9,212
Mol. weight
144.2 kDa
Ligands
GTP, MG
Released
21 Sept 2022

Explore 8DYO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8DYO contains 72 α-helices and 11 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 66 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix6-149
α-helix20-256
α-helix27-304
α-helix45-473
α-helix51-6818
α-helix78-8912
α-helix94-10714
α-helix117-12812
α-helix132-14817
α-helix154-16714
α-helix174-18714
α-helix188-1903
α-helix202-21312
α-helix218-23114
α-helix244-25411
α-helix262-27716
α-helix281-2844
α-helix288-29912
α-helix307-3093
α-helix329-34315
α-helix349-36012
α-helix366-37914
α-helix385-3895
α-helix398-4014
α-helix409-42214
α-helix427-4293
α-helix436-44510
α-helix452-46110
α-helix466-4683
α-helix471-4766
α-helix477-4848
α-helix494-51118
α-helix519-53012
α-helix538-55417
α-helix557-5593
α-helix560-5634
α-helix569-5724
α-helix586-59712
α-helix606-61611
α-helix671-68212
α-helix692-70413
α-helix711-72111
α-helix724-73310
α-helix738-75619
α-helix766-78015
α-helix784-7874
α-helix795-8039
α-helix828-8347
α-helix835-8373
α-helix843-8453
α-helix852-8543
α-helix855-86612
α-helix872-88817
α-helix891-90717
α-helix917-92711
α-helix932-9365
α-helix938-9414
α-helix946-9516
α-helix955-97016
α-helix981-9877
α-helix998-10047
α-helix1020-103011
α-helix1038-10403
α-helix1046-10483
α-helix1060-10645
α-helix1070-10723
Chain B: 6 helices, 11 β-strands
ElementResiduesLengthSheet
β-strand12-1321
β-strand16-1832
α-helix25-339
β-strand4613
β-strand52-5651
β-strand59-6351
β-strand6913
α-helix80-823
β-strand88-9032
β-strand9314
α-helix97-11317
β-strand119-12132
β-strand12414
α-helix135-1373
α-helix140-1434
β-strand147-14822
α-helix161-17111

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Importin-4Aprotein1081Homo sapiensQ8TEX9 (AlphaFold model)
GTP-binding nuclear protein GSP1/CNR1Bprotein219Saccharomyces cerevisiaeP32835 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8DYO_1 Importin-4 (chains A)
MESAGLEQLLRELLLPDTERIRRATEQLQIVLRAPAALPALCDLLASAADPQIRQFAAVL
TRRRLNTRWRRLAAEQRESLKSLILTALQRETEHCVSLSLAQLSATIFRKEGLEAWPQLL
QLLQHSTHSPHSPEREMGLLLLSVVVTSRPEAFQPHHRELLRLLNETLGEVGSPGLLFYS
LRTLTTMAPYLSTEDVPLARMLVPKLIMAMQTLIPIDEAKACEALEALDELLESEVPVIT
PYLSEVLTFCLEVARNVALGNAIRIRILCCLTFLVKVKSKALLKNRLLPPLLHTLFPIVA
AEPPPGQLDPEDQDSEEEELEIELMGETPKHFAVQVVDMLALHLPPEKLCPQLMPMLEEA
LRSESPYQRKAGLLVLAVLSDGAGDHIRQRLLPPLLQIVCKGLEDPSQVVRNAALFALGQ
FSENLQPHISSYSREVMPLLLAYLKSVPLGHTHHLAKACYALENFVENLGPKVQPYLPEL
MECMLQLLRNPSSPRAKELAVSALGAIATAAQASLLPYFPAIMEHLREFLLTGREDLQPV
QIQSLETLGVLARAVGEPMRPLAEECCQLGLGLCDQVDDPDLRRCTYSLFAALSGLMGEG
LAPHLEQITTLMLLSLRSTEGIVPQYDGSSSFLLFDDESDGEEEEELMDEDVEEEDDSEI
SGYSVENAFFDEKEDTCAAVGEISVNTSVAFLPYMESVFEEVFKLLECPHLNVRKAAHEA
LGQFCCALHKACQSCPSEPNTAALQAALARVVPSYMQAVNRERERQVVMAVLEALTGVLR
SCGTLTLKPPGRLAELCGVLKAVLQRKTACQDTDEEEEEEDDDQAEYDAMLLEHAGEAIP
ALAAAAGGDSFAPFFAGFLPLLVCKTKQGCTVAEKSFAVGTLAETIQGLGAASAQFVSRL
LPVLLSTAQEADPEVRSNAIFGMGVLAEHGGHPAQEHFPKLLGLLFPLLARERHDRVRDN
ICGALARLLMASPTRKPEPQVLAALLHALPLKEDLEEWVTIGRLFSFLYQSSPDQVIDVA
PELLRICSLILADNKIPPDTKAALLLLLTFLAKQHTDSFQAALGSLPVDKAQELQAVLGL
S
Sequence of entity 2 (B), FASTA
>8DYO_2 GTP-binding nuclear protein GSP1/CNR1 (chains B)
MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGE
IKFDVWDTAGLEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIV
LCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVA
SPALAPPEVQVDEQLMQQYQQEMEQATALPLPDEDDADL

Ligands and cofactors

IDNameFormulaCopies
GTPGuanosine-5'-triphosphateC10 H16 N5 O14 P31
MGMagnesium ionMg1

Primary citation

Structure of IMPORTIN-4 bound to the H3-H4-ASF1 histone-histone chaperone complex. Bernardes, N.E., Fung, H.Y.J., Li, Y. et al. Proc Natl Acad Sci U S A (2022) 119:e2207177119-e2207177119. DOI 10.1073/pnas.2207177119 · PubMed

Other PDB entries of the same protein (UniProt Q8TEX9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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