7UXH: MTORC1-TFEB-Rag-Ragulator complex
cryo-EM structure of the mTORC1-TFEB-Rag-Ragulator complex. Determined by electron microscopy at 3.2 Å resolution. Released 30 Nov 2022.
- Method
- Electron microscopy
- Resolution
- 3.2 Å
- Organism
- Homo sapiens
- Chains
- 36
- Atoms
- 95,166
- Mol. weight
- 1644.64 kDa
- Ligands
- IHP, GDP, MG, GTP
- Released
- 30 Nov 2022
Explore 7UXH in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7UXH contains 582 α-helices and 345 β-strands across 36 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains a, h and K: 2 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-9 | 4 | |
| β-strand | 17-19 | 3 | 4 |
| β-strand | 22 | 1 | 5 |
| β-strand | 25 | 1 | 5 |
| β-strand | 30-31 | 2 | 4 |
| α-helix | 38-50 | 13 | |
| β-strand | 65-69 | 5 | 4 |
| β-strand | 73-79 | 7 | 4 |
| β-strand | 84-90 | 7 | 4 |
Chain A: 137 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 20-28 | 9 | |
| α-helix | 36-47 | 12 | |
| α-helix | 62-76 | 15 | |
| α-helix | 80-92 | 13 | |
| α-helix | 101-114 | 14 | |
| α-helix | 120-134 | 15 | |
| α-helix | 141-156 | 16 | |
| α-helix | 163-178 | 16 | |
| α-helix | 182-186 | 5 | |
| α-helix | 187-198 | 12 | |
| α-helix | 203-222 | 20 | |
| α-helix | 234-244 | 11 | |
| α-helix | 258-275 | 18 | |
| α-helix | 277-289 | 13 | |
| α-helix | 360-368 | 9 | |
| α-helix | 370-379 | 10 | |
| α-helix | 380-382 | 3 | |
| α-helix | 386-402 | 17 | |
| α-helix | 404-409 | 6 | |
| α-helix | 412-425 | 14 | |
| α-helix | 430-443 | 14 | |
| α-helix | 452-461 | 10 | |
| α-helix | 481-493 | 13 | |
| α-helix | 495-497 | 3 | |
| α-helix | 498-511 | 14 | |
| α-helix | 515-527 | 13 | |
| α-helix | 529-531 | 3 | |
| α-helix | 532-547 | 16 | |
| α-helix | 580-591 | 12 | |
| α-helix | 600-612 | 13 | |
| α-helix | 616-629 | 14 | |
| α-helix | 646-665 | 20 | |
| α-helix | 669-678 | 10 | |
| α-helix | 681-683 | 3 | |
| α-helix | 684-687 | 4 | |
| α-helix | 690-699 | 10 | |
| α-helix | 705-717 | 13 | |
| α-helix | 723-742 | 20 | |
| α-helix | 747-763 | 17 | |
| α-helix | 772-783 | 12 | |
| α-helix | 792-809 | 18 | |
| α-helix | 810-816 | 7 | |
| α-helix | 817-829 | 13 | |
| α-helix | 837-851 | 15 | |
| α-helix | 856-860 | 5 | |
| α-helix | 864-870 | 7 | |
| α-helix | 878-891 | 14 | |
| α-helix | 896-902 | 7 | |
| α-helix | 943-960 | 18 | |
| α-helix | 962-967 | 6 | |
| α-helix | 968-982 | 15 | |
| α-helix | 987-989 | 3 | |
| α-helix | 990-1003 | 14 | |
| α-helix | 1009-1023 | 15 | |
| α-helix | 1024-1027 | 4 | |
| α-helix | 1028-1041 | 14 | |
| α-helix | 1046-1063 | 18 | |
| α-helix | 1064-1069 | 6 | |
| α-helix | 1071-1083 | 13 | |
| α-helix | 1091-1101 | 11 | |
| α-helix | 1104-1107 | 4 | |
| α-helix | 1111-1123 | 13 | |
| α-helix | 1129-1145 | 17 | |
| α-helix | 1153-1165 | 13 | |
| α-helix | 1170-1184 | 15 | |
| α-helix | 1186-1188 | 3 | |
| α-helix | 1192-1201 | 10 | |
| α-helix | 1207-1218 | 12 | |
| α-helix | 1263-1265 | 3 | |
| α-helix | 1266-1269 | 4 | |
| α-helix | 1277-1293 | 17 | |
| α-helix | 1298-1302 | 5 | |
| α-helix | 1304-1309 | 6 | |
| α-helix | 1311-1316 | 6 | |
| α-helix | 1318-1326 | 9 | |
| α-helix | 1331-1346 | 16 | |
| α-helix | 1351-1366 | 16 | |
| α-helix | 1374-1376 | 3 | |
| α-helix | 1380-1389 | 10 | |
| α-helix | 1393-1406 | 14 | |
| α-helix | 1410-1422 | 13 | |
| α-helix | 1426-1439 | 14 | |
| α-helix | 1447-1452 | 6 | |
| α-helix | 1456-1467 | 12 | |
| α-helix | 1474-1487 | 14 | |
| α-helix | 1490-1499 | 10 | |
| α-helix | 1506-1523 | 18 | |
| α-helix | 1526-1533 | 8 | |
| α-helix | 1541-1553 | 13 | |
| α-helix | 1557-1579 | 23 | |
| α-helix | 1583-1605 | 23 | |
| α-helix | 1609-1611 | 3 | |
| α-helix | 1612-1625 | 14 | |
| α-helix | 1630-1640 | 11 | |
| α-helix | 1650-1662 | 13 | |
| α-helix | 1666-1677 | 12 | |
| α-helix | 1688-1690 | 3 | |
| α-helix | 1694-1706 | 13 | |
| α-helix | 1710-1730 | 21 | |
| α-helix | 1738-1762 | 25 | |
| α-helix | 1768-1782 | 15 | |
| α-helix | 1787-1811 | 25 | |
| α-helix | 1868-1877 | 10 | |
| α-helix | 1881-1895 | 15 | |
| α-helix | 1901-1913 | 13 | |
| α-helix | 1917-1929 | 13 | |
| α-helix | 1934-1938 | 5 | |
| α-helix | 1939-1944 | 6 | |
| α-helix | 1951-1966 | 16 | |
| α-helix | 1969-1971 | 3 | |
| α-helix | 1973-1979 | 7 | |
| α-helix | 1985-2002 | 18 | |
| α-helix | 2004-2020 | 17 | |
| α-helix | 2023-2041 | 19 | |
| α-helix | 2045-2058 | 14 | |
| α-helix | 2065-2091 | 27 | |
| α-helix | 2094-2114 | 21 | |
| β-strand | 2120-2122 | 3 | 1 |
| α-helix | 2123-2126 | 4 | |
| α-helix | 2128-2131 | 4 | |
| β-strand | 2139 | 1 | 2 |
| α-helix | 2140 | 1 | |
| α-helix | 2149-2150 | 2 | |
| β-strand | 2152 | 1 | 2 |
| β-strand | 2153-2156 | 4 | 1 |
| β-strand | 2159-2162 | 4 | 1 |
| β-strand | 2170-2176 | 7 | 1 |
| β-strand | 2181-2187 | 7 | 1 |
| α-helix | 2193-2211 | 19 | |
| α-helix | 2213-2218 | 6 | |
| β-strand | 2227-2229 | 3 | 1 |
| β-strand | 2235-2238 | 4 | 1 |
| β-strand | 2243-2245 | 3 | 3 |
| α-helix | 2246-2256 | 11 | |
| α-helix | 2263-2271 | 9 | |
| α-helix | 2275-2277 | 3 | |
| α-helix | 2280-2293 | 14 | |
| α-helix | 2298-2306 | 9 | |
| α-helix | 2310-2334 | 25 | |
| β-strand | 2344-2347 | 4 | 3 |
| β-strand | 2353-2355 | 3 | 3 |
| α-helix | 2364-2367 | 4 | |
| α-helix | 2381-2385 | 5 | |
| α-helix | 2395-2409 | 15 | |
| α-helix | 2411-2420 | 10 | |
| α-helix | 2493-2509 | 17 | |
| α-helix | 2521-2532 | 12 | |
| α-helix | 2535-2539 | 5 | |
| α-helix | 2543-2545 | 3 | |
Chain b: 3 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-13 | 11 | |
| β-strand | 21-24 | 4 | 4 |
| β-strand | 30-34 | 5 | 4 |
| α-helix | 43-51 | 9 | |
| α-helix | 52-54 | 3 | |
| β-strand | 64-68 | 5 | 4 |
| β-strand | 73-78 | 6 | 4 |
| β-strand | 83-88 | 6 | 4 |
Chains B and D: 3 helices, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 14-19 | 6 | 6 |
| β-strand | 23-27 | 5 | 6 |
| β-strand | 36-39 | 4 | 6 |
| β-strand | 47-50 | 4 | 7 |
| β-strand | 56-60 | 5 | 7 |
| β-strand | 62-69 | 8 | 7 |
| α-helix | 75-77 | 3 | |
| β-strand | 80-87 | 8 | 7 |
| β-strand | 88-93 | 6 | 8 |
| β-strand | 99-104 | 6 | 8 |
| β-strand | 108-113 | 6 | 8 |
| β-strand | 124-126 | 3 | 8 |
| α-helix | 130-131 | 2 | |
| β-strand | 134-136 | 3 | 9 |
| β-strand | 142-145 | 4 | 9 |
| β-strand | 152 | 1 | 10 |
| β-strand | 153-156 | 4 | 9 |
| β-strand | 165 | 1 | 10 |
| β-strand | 173-178 | 6 | 11 |
| β-strand | 184-189 | 6 | 11 |
| β-strand | 193-199 | 7 | 11 |
| α-helix | 200 | 1 | |
| β-strand | 210-217 | 8 | 11 |
| β-strand | 223-228 | 6 | 12 |
| β-strand | 234-239 | 6 | 12 |
| β-strand | 243-248 | 6 | 12 |
| β-strand | 254-259 | 6 | 12 |
| β-strand | 273-278 | 6 | 13 |
| β-strand | 284-289 | 6 | 13 |
| β-strand | 294-298 | 5 | 13 |
| β-strand | 304-308 | 5 | 13 |
| β-strand | 315-321 | 7 | 6 |
Chain c: 13 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 6-14 | 9 | 17 |
| α-helix | 20-28 | 9 | |
| α-helix | 33-38 | 6 | |
| β-strand | 46-51 | 6 | 17 |
| β-strand | 56-62 | 7 | 17 |
| α-helix | 67-74 | 8 | |
| α-helix | 78-82 | 5 | |
| β-strand | 85-93 | 9 | 17 |
| α-helix | 99-116 | 18 | |
| β-strand | 121-127 | 7 | 17 |
| α-helix | 129-131 | 3 | |
| α-helix | 134-151 | 18 | |
| β-strand | 157-161 | 5 | 17 |
| α-helix | 168-177 | 10 | |
| α-helix | 184-198 | 15 | |
| β-strand | 203-207 | 5 | 18 |
| β-strand | 213-217 | 5 | 18 |
| α-helix | 228-242 | 15 | |
| α-helix | 243-245 | 3 | |
| β-strand | 249-255 | 7 | 18 |
| β-strand | 260-265 | 6 | 18 |
| β-strand | 270-276 | 7 | 18 |
| α-helix | 283-297 | 15 | |
| α-helix | 298-300 | 3 | |
Chain C: 137 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 20-28 | 9 | |
| α-helix | 36-47 | 12 | |
| α-helix | 62-76 | 15 | |
| α-helix | 80-92 | 13 | |
| α-helix | 101-114 | 14 | |
| α-helix | 120-134 | 15 | |
| α-helix | 141-156 | 16 | |
| α-helix | 163-178 | 16 | |
| α-helix | 182-186 | 5 | |
| α-helix | 187-198 | 12 | |
| α-helix | 203-222 | 20 | |
| α-helix | 234-244 | 11 | |
| α-helix | 258-275 | 18 | |
| α-helix | 277-289 | 13 | |
| α-helix | 360-368 | 9 | |
| α-helix | 370-379 | 10 | |
| α-helix | 380-382 | 3 | |
| α-helix | 386-402 | 17 | |
| α-helix | 404-409 | 6 | |
| α-helix | 412-424 | 13 | |
| α-helix | 430-443 | 14 | |
| α-helix | 452-461 | 10 | |
| α-helix | 481-493 | 13 | |
| α-helix | 495-497 | 3 | |
| α-helix | 498-511 | 14 | |
| α-helix | 515-527 | 13 | |
| α-helix | 529-531 | 3 | |
| α-helix | 532-547 | 16 | |
| α-helix | 580-591 | 12 | |
| α-helix | 600-612 | 13 | |
| α-helix | 616-629 | 14 | |
| α-helix | 646-665 | 20 | |
| α-helix | 669-678 | 10 | |
| α-helix | 681-683 | 3 | |
| α-helix | 684-687 | 4 | |
| α-helix | 690-699 | 10 | |
| α-helix | 705-717 | 13 | |
| α-helix | 723-742 | 20 | |
| α-helix | 747-763 | 17 | |
| α-helix | 772-783 | 12 | |
| α-helix | 792-809 | 18 | |
| α-helix | 810-816 | 7 | |
| α-helix | 817-829 | 13 | |
| α-helix | 837-851 | 15 | |
| α-helix | 856-860 | 5 | |
| α-helix | 864-870 | 7 | |
| α-helix | 878-891 | 14 | |
| α-helix | 896-902 | 7 | |
| α-helix | 934-937 | 4 | |
| α-helix | 943-945 | 3 | |
| α-helix | 946-960 | 15 | |
| α-helix | 962-967 | 6 | |
| α-helix | 968-982 | 15 | |
| α-helix | 987-989 | 3 | |
| α-helix | 990-1003 | 14 | |
| α-helix | 1009-1023 | 15 | |
| α-helix | 1024-1030 | 7 | |
| α-helix | 1031-1041 | 11 | |
| α-helix | 1046-1063 | 18 | |
| α-helix | 1064-1069 | 6 | |
| α-helix | 1071-1083 | 13 | |
| α-helix | 1091-1101 | 11 | |
| α-helix | 1104-1107 | 4 | |
| α-helix | 1111-1123 | 13 | |
| α-helix | 1129-1145 | 17 | |
| α-helix | 1153-1165 | 13 | |
| α-helix | 1170-1184 | 15 | |
| α-helix | 1186-1188 | 3 | |
| α-helix | 1192-1201 | 10 | |
| α-helix | 1207-1218 | 12 | |
| α-helix | 1263-1265 | 3 | |
| α-helix | 1266-1269 | 4 | |
| α-helix | 1277-1293 | 17 | |
| α-helix | 1298-1302 | 5 | |
| α-helix | 1304-1309 | 6 | |
| α-helix | 1311-1316 | 6 | |
| α-helix | 1318-1326 | 9 | |
| α-helix | 1331-1346 | 16 | |
| α-helix | 1351-1366 | 16 | |
| α-helix | 1374-1376 | 3 | |
| α-helix | 1380-1390 | 11 | |
| α-helix | 1393-1406 | 14 | |
| α-helix | 1410-1422 | 13 | |
| α-helix | 1426-1439 | 14 | |
| α-helix | 1447-1452 | 6 | |
| α-helix | 1456-1467 | 12 | |
| α-helix | 1474-1487 | 14 | |
| α-helix | 1490-1499 | 10 | |
| α-helix | 1506-1523 | 18 | |
| α-helix | 1526-1533 | 8 | |
| α-helix | 1541-1553 | 13 | |
| α-helix | 1557-1579 | 23 | |
| α-helix | 1583-1605 | 23 | |
| α-helix | 1609-1611 | 3 | |
| α-helix | 1612-1625 | 14 | |
| α-helix | 1630-1640 | 11 | |
| α-helix | 1650-1662 | 13 | |
| α-helix | 1666-1677 | 12 | |
| α-helix | 1688-1690 | 3 | |
| α-helix | 1694-1706 | 13 | |
| α-helix | 1710-1730 | 21 | |
| α-helix | 1738-1762 | 25 | |
| α-helix | 1768-1782 | 15 | |
| α-helix | 1787-1811 | 25 | |
| α-helix | 1868-1877 | 10 | |
| α-helix | 1881-1895 | 15 | |
| α-helix | 1901-1913 | 13 | |
| α-helix | 1917-1929 | 13 | |
| α-helix | 1934-1938 | 5 | |
| α-helix | 1939-1944 | 6 | |
| α-helix | 1951-1966 | 16 | |
| α-helix | 1969-1971 | 3 | |
| α-helix | 1973-1979 | 7 | |
| α-helix | 1985-2002 | 18 | |
| α-helix | 2005-2020 | 16 | |
| α-helix | 2023-2041 | 19 | |
| α-helix | 2045-2058 | 14 | |
| α-helix | 2065-2091 | 27 | |
| α-helix | 2094-2114 | 21 | |
| β-strand | 2120-2122 | 3 | 14 |
| α-helix | 2123-2126 | 4 | |
| α-helix | 2128-2131 | 4 | |
| β-strand | 2139 | 1 | 15 |
| α-helix | 2140 | 1 | |
| α-helix | 2149-2150 | 2 | |
| β-strand | 2152 | 1 | 15 |
| β-strand | 2153-2156 | 4 | 14 |
| β-strand | 2159-2162 | 4 | 14 |
| β-strand | 2170-2176 | 7 | 14 |
| β-strand | 2181-2187 | 7 | 14 |
| α-helix | 2193-2211 | 19 | |
| α-helix | 2213-2218 | 6 | |
| β-strand | 2227-2229 | 3 | 14 |
| β-strand | 2235-2238 | 4 | 14 |
| β-strand | 2243-2245 | 3 | 16 |
| α-helix | 2246-2256 | 11 | |
| α-helix | 2263-2271 | 9 | |
| α-helix | 2275-2277 | 3 | |
| α-helix | 2280-2293 | 14 | |
| α-helix | 2298-2306 | 9 | |
| α-helix | 2310-2334 | 25 | |
| β-strand | 2344-2347 | 4 | 16 |
| β-strand | 2353-2355 | 3 | 16 |
| α-helix | 2381-2385 | 5 | |
| α-helix | 2395-2409 | 15 | |
| α-helix | 2411-2420 | 10 | |
| α-helix | 2493-2509 | 17 | |
| α-helix | 2521-2532 | 12 | |
| α-helix | 2535-2539 | 5 | |
Chain d: 10 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 63-67 | 5 | 27 |
| α-helix | 74-82 | 9 | |
| β-strand | 111-114 | 4 | 27 |
| β-strand | 137-145 | 9 | 27 |
| α-helix | 151-167 | 17 | |
| β-strand | 172-178 | 7 | 27 |
| α-helix | 180-182 | 3 | |
| α-helix | 185-203 | 19 | |
| β-strand | 214-218 | 5 | 27 |
| α-helix | 224-233 | 10 | |
| α-helix | 240-254 | 15 | |
| β-strand | 258-263 | 6 | 18 |
| β-strand | 268-272 | 5 | 18 |
| α-helix | 276-277 | 2 | |
| α-helix | 279-298 | 20 | |
| β-strand | 315-320 | 6 | 18 |
| β-strand | 324-330 | 7 | 18 |
| β-strand | 335-342 | 8 | 18 |
| α-helix | 343-346 | 4 | |
| α-helix | 349-366 | 18 | |
Chains e and O: 7 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 49-64 | 16 | |
| α-helix | 66-67 | 2 | |
| α-helix | 68-70 | 3 | |
| α-helix | 78-98 | 21 | |
| α-helix | 105-109 | 5 | |
| α-helix | 116-119 | 4 | |
| α-helix | 126-144 | 19 | |
24 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Serine/threonine-protein kinase mTOR | A, C | protein | 2549 | Homo sapiens | P42345 (AlphaFold model) |
| Target of rapamycin complex subunit LST8 | B, D | protein | 326 | Homo sapiens | Q9BVC4 (AlphaFold model) |
| Regulatory-associated protein of mTOR | E, U | protein | 1335 | Homo sapiens | Q8N122 (AlphaFold model) |
| Ras-related GTP-binding protein A | F, M, V, c | protein | 313 | Homo sapiens | Q7L523 (AlphaFold model) |
| Ras-related GTP-binding protein C | G, N, W, d | protein | 399 | Homo sapiens | Q9HB90 |
| Ragulator complex protein LAMTOR1 | H, O, X, e | protein | 161 | Homo sapiens | Q6IAA8 |
| Ragulator complex protein LAMTOR2 | I, P, Y, f | protein | 125 | Homo sapiens | Q9Y2Q5 |
| Ragulator complex protein LAMTOR3 | J, Q, Z, g | protein | 124 | Homo sapiens | Q9UHA4 |
| Ragulator complex protein LAMTOR4 | K, R, a, h | protein | 99 | Homo sapiens | Q0VGL1 |
| Ragulator complex protein LAMTOR5 | L, S, b, i | protein | 91 | Homo sapiens | O43504 |
| Transcription factor EB | T, j | protein | 476 | Homo sapiens | P19484 |
Sequence of entity 1 (A, C), FASTA
>7UXH_1 Serine/threonine-protein kinase mTOR (chains A, C)
MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEES
TRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDP
VVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVP
TFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEE
AEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
KDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESR
CCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHV
LSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDA
TVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLL
KMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLT
QFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLL
VVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSS
MNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALI
LKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVA
LWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHK
VNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFR
DQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLV
SFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLR
VFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVD
RLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLV
RHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHV
STINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNA
AFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGI
VLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHF
GELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEK
WTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLA
QQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
ERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVD
PSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHK
QELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEA
VLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSP
TPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVN
EALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVAS
KSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFG
ERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQA
WDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSL
QVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNL
SIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTL
MQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRH
PSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITC
HTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDG
VELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLD
VPTQVELLIKQATSHENLCQCYIGWCPFW
Sequence of entity 2 (B, D), FASTA
>7UXH_2 Target of rapamycin complex subunit LST8 (chains B, D)
MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAA
GYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQ
CQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDP
DASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSA
DQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVE
TGEIKREYGGHQKAVVCLAFNDSVLG
Sequence of entity 3 (E, U), FASTA
>7UXH_3 Regulatory-associated protein of mTOR (chains E, U)
MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSV
ALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYK
QSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIY
DLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNC
IQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPG
RLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSY
NCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWL
TMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLL
QSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILA
VIVNSYHTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSA
HEKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLVSDGSPMVR
KELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTEGGSLTPVRDSPCTPRLRSV
SSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLGSPENEEHILS
FETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYK
ATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRD
LPSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFC
DWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLN
RNPGVPSVVKFHPFTPCIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQD
CSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMS
SGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALS
ECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALD
IHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGS
NDYYISVYSVEKRVR
Sequence of entity 4 (F, M, V, c), FASTA
>7UXH_4 Ras-related GTP-binding protein A (chains F, M, V, c)
MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNL
WDCGGLDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDA
KIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQL
IPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKL
SCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKL
ERVDGPKHSLLMR
Sequence of entity 5 (G, N, W, d), FASTA
>7UXH_5 Ras-related GTP-binding protein C (chains G, N, W, d)
MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGPGGADSS
KPRILLMGLRRSGKNSIQKVVFHKMSPNETLFLESTNKIYKDDISNSSFVNFQIWDFPGQ
MDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKV
DGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQL
PTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYG
LKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFR
KAIHEVFEVGVTSHRSCGHQTSASSLKALTHNGTPRNAI
Sequence of entity 6 (H, O, X, e), FASTA
>7UXH_6 Ragulator complex protein LAMTOR1 (chains H, O, X, e)
MGCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAK
TASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLA
SEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP
Sequence of entity 7 (I, P, Y, f), FASTA
>7UXH_7 Ragulator complex protein LAMTOR2 (chains I, P, Y, f)
MLRPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNG
NQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLT
QVAAS
Sequence of entity 8 (J, Q, Z, g), FASTA
>7UXH_8 Ragulator complex protein LAMTOR3 (chains J, Q, Z, g)
MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDNAPEHALRPGFLSTFALATDQG
SKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSSANTGLIVSLEKELAPLFEELRQV
VEVS
Sequence of entity 9 (K, R, a, h), FASTA
>7UXH_9 Ragulator complex protein LAMTOR4 (chains K, R, a, h)
MTSALTQGLERIPDQLGYLVLSEGAVLASSGDLENDEQAASAISELVSTACGFRLHRGMN
VPFKRLSVVFGEHTLLVTVSGQRVFVVKRQNRGREPIDV
Sequence of entity 10 (L, S, b, i), FASTA
>7UXH_10 Ragulator complex protein LAMTOR5 (chains L, S, b, i)
MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPT
DIPVVCLESDNGNIMIQKHDGITVAVHKMAS
Sequence of entity 11 (T, j), FASTA
>7UXH_11 Transcription factor EB (chains T, j)
MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQ
SPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPA
ASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQM
PNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNH
NLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELEN
HSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEAL
MLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPG
HGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| IHP | Inositol hexakisphosphate | C6 H18 O24 P6 | 2 |
| GDP | Guanosine-5'-diphosphate | C10 H15 N5 O11 P2 | 4 |
| MG | Magnesium ion | Mg | 4 |
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 4 |
Primary citation
Structure of the lysosomal mTORC1-TFEB-Rag-Ragulator megacomplex. Cui, Z., Napolitano, G., de Araujo, M.E.G. et al. Nature (2023) 614:572-579. DOI 10.1038/s41586-022-05652-7 · PubMed
Other PDB entries of the same protein (UniProt P42345 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 4DRI 1.45 Å, Co-crystal structure of the PPIase domain of FKBP51, Rapamycin and the FRB fragment of…
- 9DBO 1.55 Å, Crystal structure of a synthetic Fab (R3E9) in complex with the FRB domain of mTOR
- 5GPG 1.67 Å, Co-crystal structure of the FK506 binding domain of human FKBP25, Rapamycin and the FRB…
- 5WBH 1.75 Å, Structure of the FRB domain of mTOR bound to a substrate recruitment peptide of S6K1
- 4DRJ 1.8 Å, o-crystal structure of the PPIase domain of FKBP52, Rapamycin and the FRB fragment of mTOR
- 3FAP 1.85 Å, Atomic structures of the rapamycin analogs in complex with both human FKBP12 and frb…
- 8PPZ 1.85 Å, Co-crystal structure of FKBP12, compound 7 and the FRB fragment of mTOR
- 8XI9 1.85 Å, Crystal structure of FRB-FKBP fusion protein in complex with rapamycin
- 9PIW 1.9 Å, Crystal structure of a synthetic Fab (1A) in complex with the FRB domain of mTOR
- 9DL0 2.0 Å, Crystal structure of a synthetic Fab (R3H8) in complex with the FRB domain of mTOR
- 9PJ1 2.05 Å, Crystal structure of a synthetic Fab (4R) in complex with the ternary assembly of…
- 9PIU 2.18 Å, Crystal structure of a synthetic Fab (2C) in complex with the FRB domain of mTOR
Browse structure collections
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